Flow cytometric identification of Mamiellales clade II in the Southern Atlantic Ocean
Flow cytometric sorting, based on cellular optical properties and macromolecule content, has been successfully employed to taxonomically affiliate bacterioplankton. However, this approach has not been much used for eukaryotic plankton. To redress this imbalance, we identified a conspicuous group of...
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ftpubman:oai:pure.mpg.de:item_2484532 2023-08-20T04:09:49+02:00 Flow cytometric identification of Mamiellales clade II in the Southern Atlantic Ocean Gómez-Pereira, P. Kennaway, G. Fuchs, B. Tarran, G. Zubkov, M. 2013-03 application/pdf http://hdl.handle.net/21.11116/0000-0001-C711-B http://hdl.handle.net/21.11116/0000-0006-F4B1-E eng eng http://hdl.handle.net/21.11116/0000-0001-C711-B http://hdl.handle.net/21.11116/0000-0006-F4B1-E FEMS Microbiology Ecology info:eu-repo/semantics/article 2013 ftpubman 2023-08-01T20:47:08Z Flow cytometric sorting, based on cellular optical properties and macromolecule content, has been successfully employed to taxonomically affiliate bacterioplankton. However, this approach has not been much used for eukaryotic plankton. To redress this imbalance, we identified a conspicuous group of red autofluorescent picoplankton in surface waters of the South Atlantic Ocean. Using catalysed reporter deposition fluorescence in situ hybridization, virtually, all cells sorted from that group were affiliated with the Mamiellales clade II (84 +/- 4%, division Chlorophyta) with a size of 1.6 +/- 0.03m. Based on electron microscopy, the Mamiellales clade IIsorted cells have a simple morphology with apparently no scales, flagella or surface features. Their latitudinal distribution resembled the distribution of Synechococcus with very low concentrations in the surface waters of the Southern subtropical gyre (0.61.6x103cellsmL1) and increased concentrations in the Southern temperate waters 8.3x103cellsmL1. Identification of the flow cytometric group as Mamiellales clade II allowed us to characterize the morphology of these enigmatic uncultured picoplanktonic cells by electron microscopy and to determine their apparent preference for temperate rather than subtropical oceanic photic waters. Article in Journal/Newspaper South Atlantic Ocean Max Planck Society: MPG.PuRe |
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Max Planck Society: MPG.PuRe |
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English |
description |
Flow cytometric sorting, based on cellular optical properties and macromolecule content, has been successfully employed to taxonomically affiliate bacterioplankton. However, this approach has not been much used for eukaryotic plankton. To redress this imbalance, we identified a conspicuous group of red autofluorescent picoplankton in surface waters of the South Atlantic Ocean. Using catalysed reporter deposition fluorescence in situ hybridization, virtually, all cells sorted from that group were affiliated with the Mamiellales clade II (84 +/- 4%, division Chlorophyta) with a size of 1.6 +/- 0.03m. Based on electron microscopy, the Mamiellales clade IIsorted cells have a simple morphology with apparently no scales, flagella or surface features. Their latitudinal distribution resembled the distribution of Synechococcus with very low concentrations in the surface waters of the Southern subtropical gyre (0.61.6x103cellsmL1) and increased concentrations in the Southern temperate waters 8.3x103cellsmL1. Identification of the flow cytometric group as Mamiellales clade II allowed us to characterize the morphology of these enigmatic uncultured picoplanktonic cells by electron microscopy and to determine their apparent preference for temperate rather than subtropical oceanic photic waters. |
format |
Article in Journal/Newspaper |
author |
Gómez-Pereira, P. Kennaway, G. Fuchs, B. Tarran, G. Zubkov, M. |
spellingShingle |
Gómez-Pereira, P. Kennaway, G. Fuchs, B. Tarran, G. Zubkov, M. Flow cytometric identification of Mamiellales clade II in the Southern Atlantic Ocean |
author_facet |
Gómez-Pereira, P. Kennaway, G. Fuchs, B. Tarran, G. Zubkov, M. |
author_sort |
Gómez-Pereira, P. |
title |
Flow cytometric identification of Mamiellales clade II in the Southern Atlantic Ocean |
title_short |
Flow cytometric identification of Mamiellales clade II in the Southern Atlantic Ocean |
title_full |
Flow cytometric identification of Mamiellales clade II in the Southern Atlantic Ocean |
title_fullStr |
Flow cytometric identification of Mamiellales clade II in the Southern Atlantic Ocean |
title_full_unstemmed |
Flow cytometric identification of Mamiellales clade II in the Southern Atlantic Ocean |
title_sort |
flow cytometric identification of mamiellales clade ii in the southern atlantic ocean |
publishDate |
2013 |
url |
http://hdl.handle.net/21.11116/0000-0001-C711-B http://hdl.handle.net/21.11116/0000-0006-F4B1-E |
genre |
South Atlantic Ocean |
genre_facet |
South Atlantic Ocean |
op_source |
FEMS Microbiology Ecology |
op_relation |
http://hdl.handle.net/21.11116/0000-0001-C711-B http://hdl.handle.net/21.11116/0000-0006-F4B1-E |
_version_ |
1774723531068669952 |