TEM preparation methods and influence of radiation damage on the beam sensitive CaCO 3 shell of Emiliania huxleyi

The ultrastructure of biologically formed calcium carbonate crystals like the shell of Emiliania huxleyi depends on the environmental conditions such as pH value, temperature and salinity. Therefore, they can be used as indicator for climate changes. However, for this a detailed understanding of the...

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Published in:Micron
Main Authors: Hoffmann, R., Wochnik, A., Betzler, S., Matich, S., Griesshaber, E., Schmahl, W., Scheu, C.
Format: Article in Journal/Newspaper
Language:English
Published: 2014
Subjects:
Online Access:http://hdl.handle.net/11858/00-001M-0000-0024-BC46-1
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spelling ftpubman:oai:pure.mpg.de:item_2088401 2024-09-15T18:14:06+00:00 TEM preparation methods and influence of radiation damage on the beam sensitive CaCO 3 shell of Emiliania huxleyi Hoffmann, R. Wochnik, A. Betzler, S. Matich, S. Griesshaber, E. Schmahl, W. Scheu, C. 2014-07 http://hdl.handle.net/11858/00-001M-0000-0024-BC46-1 eng eng info:eu-repo/semantics/altIdentifier/doi/10.1016/j.micron.2014.03.004 http://hdl.handle.net/11858/00-001M-0000-0024-BC46-1 Micron info:eu-repo/semantics/article 2014 ftpubman https://doi.org/10.1016/j.micron.2014.03.004 2024-07-31T09:31:29Z The ultrastructure of biologically formed calcium carbonate crystals like the shell of Emiliania huxleyi depends on the environmental conditions such as pH value, temperature and salinity. Therefore, they can be used as indicator for climate changes. However, for this a detailed understanding of their crystal structure and chemical composition is required. High resolution methods like transmission electron microscopy can provide those information on the nanoscale, given that sufficiently thin samples can be prepared. In our study, we developed sample preparation techniques for cross-section and plan-view investigations and studied the sample stability under electron bombardment. In addition to the biological material (Emiliania huxleyi) we also prepared mineralogical samples (Iceland spar) for comparison. High resolution transmission electron microscopy imaging, electron diffraction and electron energy-loss spectroscopy studies revealed that all prepared samples are relatively stable under electron bombardment at an acceleration voltage of 300 kV when using a parallel illumination. Above an accumulated dose of similar to 10(5) e/nm(2) the material - independent whether its origin is biological or geological - transformed to poly-crystalline calcium oxide. (c) 2014 Elsevier Ltd. All rights reserved. Article in Journal/Newspaper Iceland Max Planck Society: MPG.PuRe Micron 62 28 36
institution Open Polar
collection Max Planck Society: MPG.PuRe
op_collection_id ftpubman
language English
description The ultrastructure of biologically formed calcium carbonate crystals like the shell of Emiliania huxleyi depends on the environmental conditions such as pH value, temperature and salinity. Therefore, they can be used as indicator for climate changes. However, for this a detailed understanding of their crystal structure and chemical composition is required. High resolution methods like transmission electron microscopy can provide those information on the nanoscale, given that sufficiently thin samples can be prepared. In our study, we developed sample preparation techniques for cross-section and plan-view investigations and studied the sample stability under electron bombardment. In addition to the biological material (Emiliania huxleyi) we also prepared mineralogical samples (Iceland spar) for comparison. High resolution transmission electron microscopy imaging, electron diffraction and electron energy-loss spectroscopy studies revealed that all prepared samples are relatively stable under electron bombardment at an acceleration voltage of 300 kV when using a parallel illumination. Above an accumulated dose of similar to 10(5) e/nm(2) the material - independent whether its origin is biological or geological - transformed to poly-crystalline calcium oxide. (c) 2014 Elsevier Ltd. All rights reserved.
format Article in Journal/Newspaper
author Hoffmann, R.
Wochnik, A.
Betzler, S.
Matich, S.
Griesshaber, E.
Schmahl, W.
Scheu, C.
spellingShingle Hoffmann, R.
Wochnik, A.
Betzler, S.
Matich, S.
Griesshaber, E.
Schmahl, W.
Scheu, C.
TEM preparation methods and influence of radiation damage on the beam sensitive CaCO 3 shell of Emiliania huxleyi
author_facet Hoffmann, R.
Wochnik, A.
Betzler, S.
Matich, S.
Griesshaber, E.
Schmahl, W.
Scheu, C.
author_sort Hoffmann, R.
title TEM preparation methods and influence of radiation damage on the beam sensitive CaCO 3 shell of Emiliania huxleyi
title_short TEM preparation methods and influence of radiation damage on the beam sensitive CaCO 3 shell of Emiliania huxleyi
title_full TEM preparation methods and influence of radiation damage on the beam sensitive CaCO 3 shell of Emiliania huxleyi
title_fullStr TEM preparation methods and influence of radiation damage on the beam sensitive CaCO 3 shell of Emiliania huxleyi
title_full_unstemmed TEM preparation methods and influence of radiation damage on the beam sensitive CaCO 3 shell of Emiliania huxleyi
title_sort tem preparation methods and influence of radiation damage on the beam sensitive caco 3 shell of emiliania huxleyi
publishDate 2014
url http://hdl.handle.net/11858/00-001M-0000-0024-BC46-1
genre Iceland
genre_facet Iceland
op_source Micron
op_relation info:eu-repo/semantics/altIdentifier/doi/10.1016/j.micron.2014.03.004
http://hdl.handle.net/11858/00-001M-0000-0024-BC46-1
op_doi https://doi.org/10.1016/j.micron.2014.03.004
container_title Micron
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container_start_page 28
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