Polyvalent cation receptor proteins (CaRs) are salinity sensors in fish
To determine whether calcium polyvalent cation-sensing receptors (CaRs) are salinity sensors in fish, we used a homology-based cloning strategy to isolate a 4.1-kb cDNA encoding a 1,027-aa dogfish shark (Squalus acanthias) kidney CaR. Expression studies in human embryonic kidney cells reveal that sh...
Published in: | Proceedings of the National Academy of Sciences |
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ftpubman:oai:pure.mpg.de:item_1813747 2023-08-20T04:05:18+02:00 Polyvalent cation receptor proteins (CaRs) are salinity sensors in fish Proc. Natl. Acad. Sci. U. S. A. Nearing, J. Betka, M. Quinn, S. Hentschel, H. Elger, M. Baum, M. Bai, M. Chattopadyhay, N. Brown, E. Hebert, S. Harris, H. 2002-07-01 http://hdl.handle.net/11858/00-001M-0000-0014-0E48-C eng eng info:eu-repo/semantics/altIdentifier/urn/http://dx.doi.org/10.1073/pnas.152294399 http://hdl.handle.net/11858/00-001M-0000-0014-0E48-C Proceedings of the National Academy of Sciences of the United States of America info:eu-repo/semantics/article 2002 ftpubman https://doi.org/10.1073/pnas.152294399 2023-08-01T20:23:05Z To determine whether calcium polyvalent cation-sensing receptors (CaRs) are salinity sensors in fish, we used a homology-based cloning strategy to isolate a 4.1-kb cDNA encoding a 1,027-aa dogfish shark (Squalus acanthias) kidney CaR. Expression studies in human embryonic kidney cells reveal that shark kidney senses combinations of Ca2+, Mg2+, and Na+ ions at concentrations present in seawater and kidney tubules. Shark kidney is expressed in multiple shark osmoregulatory organs, including specific tubules of the kidney, rectal gland, stomach, intestine, olfactory lamellae, gill, and brain. Reverse transcriptase-PCR amplification using specific primers in two teleost fish, winter flounder (Pleuronectes americanus) and Atlantic salmon (Salmo salar), reveals a similar pattern of CaR tissue expression. Exposure of the lumen of winter flounder urinary bladder to the CaR agonists, Gd3+ and neomycin, reversibly inhibit volume transport, which is important for euryhaline teleost survival in seawater. Within 24-72 hr after transfer of freshwater-adapted Atlantic salmon to seawater, there are increases in their plasma Ca2+, Mg2+, and Na+ that likely serve as a signal for internal CaRs, i.e., brain, to sense alterations in salinity in the surrounding water. We conclude that CaRs act as salinity sensors in both teleost and elasmobranch fish. Their tissue expression patterns in fish provide insights into CaR functions in terrestrial animals including humans. Article in Journal/Newspaper Atlantic salmon Salmo salar Squalus acanthias Max Planck Society: MPG.PuRe Proceedings of the National Academy of Sciences 99 14 9231 9236 |
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English |
description |
To determine whether calcium polyvalent cation-sensing receptors (CaRs) are salinity sensors in fish, we used a homology-based cloning strategy to isolate a 4.1-kb cDNA encoding a 1,027-aa dogfish shark (Squalus acanthias) kidney CaR. Expression studies in human embryonic kidney cells reveal that shark kidney senses combinations of Ca2+, Mg2+, and Na+ ions at concentrations present in seawater and kidney tubules. Shark kidney is expressed in multiple shark osmoregulatory organs, including specific tubules of the kidney, rectal gland, stomach, intestine, olfactory lamellae, gill, and brain. Reverse transcriptase-PCR amplification using specific primers in two teleost fish, winter flounder (Pleuronectes americanus) and Atlantic salmon (Salmo salar), reveals a similar pattern of CaR tissue expression. Exposure of the lumen of winter flounder urinary bladder to the CaR agonists, Gd3+ and neomycin, reversibly inhibit volume transport, which is important for euryhaline teleost survival in seawater. Within 24-72 hr after transfer of freshwater-adapted Atlantic salmon to seawater, there are increases in their plasma Ca2+, Mg2+, and Na+ that likely serve as a signal for internal CaRs, i.e., brain, to sense alterations in salinity in the surrounding water. We conclude that CaRs act as salinity sensors in both teleost and elasmobranch fish. Their tissue expression patterns in fish provide insights into CaR functions in terrestrial animals including humans. |
format |
Article in Journal/Newspaper |
author |
Nearing, J. Betka, M. Quinn, S. Hentschel, H. Elger, M. Baum, M. Bai, M. Chattopadyhay, N. Brown, E. Hebert, S. Harris, H. |
spellingShingle |
Nearing, J. Betka, M. Quinn, S. Hentschel, H. Elger, M. Baum, M. Bai, M. Chattopadyhay, N. Brown, E. Hebert, S. Harris, H. Polyvalent cation receptor proteins (CaRs) are salinity sensors in fish |
author_facet |
Nearing, J. Betka, M. Quinn, S. Hentschel, H. Elger, M. Baum, M. Bai, M. Chattopadyhay, N. Brown, E. Hebert, S. Harris, H. |
author_sort |
Nearing, J. |
title |
Polyvalent cation receptor proteins (CaRs) are salinity sensors in fish |
title_short |
Polyvalent cation receptor proteins (CaRs) are salinity sensors in fish |
title_full |
Polyvalent cation receptor proteins (CaRs) are salinity sensors in fish |
title_fullStr |
Polyvalent cation receptor proteins (CaRs) are salinity sensors in fish |
title_full_unstemmed |
Polyvalent cation receptor proteins (CaRs) are salinity sensors in fish |
title_sort |
polyvalent cation receptor proteins (cars) are salinity sensors in fish |
publishDate |
2002 |
url |
http://hdl.handle.net/11858/00-001M-0000-0014-0E48-C |
genre |
Atlantic salmon Salmo salar Squalus acanthias |
genre_facet |
Atlantic salmon Salmo salar Squalus acanthias |
op_source |
Proceedings of the National Academy of Sciences of the United States of America |
op_relation |
info:eu-repo/semantics/altIdentifier/urn/http://dx.doi.org/10.1073/pnas.152294399 http://hdl.handle.net/11858/00-001M-0000-0014-0E48-C |
op_doi |
https://doi.org/10.1073/pnas.152294399 |
container_title |
Proceedings of the National Academy of Sciences |
container_volume |
99 |
container_issue |
14 |
container_start_page |
9231 |
op_container_end_page |
9236 |
_version_ |
1774715788762021888 |