Proteomic characterization of testicular protein expression in male hornyhead turbot exhibiting high plasma estrogen levels
In selected locations offshore of urban southern California, male hornyhead turbot (Pleuronichthys verticalis) exhibit elevated plasma concentrations of the female sex steroid, 17β-estradiol (E2). Males sampled from Santa Monica Bay have consistently elevated E2 concentration (>1000 pg/ml), as mu...
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California State University, Long Beach
2014
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ftproquest:oai:pqdtoai.proquest.com:1569889 2023-05-15T18:41:08+02:00 Proteomic characterization of testicular protein expression in male hornyhead turbot exhibiting high plasma estrogen levels Larsen, Cody David 2014-01-01 00:00:01.0 http://pqdtopen.proquest.com/#viewpdf?dispub=1569889 ENG eng California State University, Long Beach http://pqdtopen.proquest.com/#viewpdf?dispub=1569889 Toxicology|Surgery|Endocrinology thesis 2014 ftproquest 2021-03-13T17:36:35Z In selected locations offshore of urban southern California, male hornyhead turbot (Pleuronichthys verticalis) exhibit elevated plasma concentrations of the female sex steroid, 17β-estradiol (E2). Males sampled from Santa Monica Bay have consistently elevated E2 concentration (>1000 pg/ml), as much as 10 times higher than males from locations offshore of Orange County. Since estrogens, including E2, are at undetectable levels in the ocean discharge of regional WWTPs, it was of interest to determine whether testicular expression of steroidogenic enzymes and other proteins impacting estrogen production may be altered and linked to endogenous E2 production. In this study, proteomics was used to screen for changes in testicular protein expression. In addition, proteomes of fish experimentally treated with E2 were compared to a control. These experiments resulted in the molecular characterization of 30 unique proteins, and provided some insight into potential underlying molecular mechanisms associated with this aberrant E2 phenotype. Thesis Turbot PQDT Open: Open Access Dissertations and Theses (ProQuest) |
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Open Polar |
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PQDT Open: Open Access Dissertations and Theses (ProQuest) |
op_collection_id |
ftproquest |
language |
English |
topic |
Toxicology|Surgery|Endocrinology |
spellingShingle |
Toxicology|Surgery|Endocrinology Larsen, Cody David Proteomic characterization of testicular protein expression in male hornyhead turbot exhibiting high plasma estrogen levels |
topic_facet |
Toxicology|Surgery|Endocrinology |
description |
In selected locations offshore of urban southern California, male hornyhead turbot (Pleuronichthys verticalis) exhibit elevated plasma concentrations of the female sex steroid, 17β-estradiol (E2). Males sampled from Santa Monica Bay have consistently elevated E2 concentration (>1000 pg/ml), as much as 10 times higher than males from locations offshore of Orange County. Since estrogens, including E2, are at undetectable levels in the ocean discharge of regional WWTPs, it was of interest to determine whether testicular expression of steroidogenic enzymes and other proteins impacting estrogen production may be altered and linked to endogenous E2 production. In this study, proteomics was used to screen for changes in testicular protein expression. In addition, proteomes of fish experimentally treated with E2 were compared to a control. These experiments resulted in the molecular characterization of 30 unique proteins, and provided some insight into potential underlying molecular mechanisms associated with this aberrant E2 phenotype. |
format |
Thesis |
author |
Larsen, Cody David |
author_facet |
Larsen, Cody David |
author_sort |
Larsen, Cody David |
title |
Proteomic characterization of testicular protein expression in male hornyhead turbot exhibiting high plasma estrogen levels |
title_short |
Proteomic characterization of testicular protein expression in male hornyhead turbot exhibiting high plasma estrogen levels |
title_full |
Proteomic characterization of testicular protein expression in male hornyhead turbot exhibiting high plasma estrogen levels |
title_fullStr |
Proteomic characterization of testicular protein expression in male hornyhead turbot exhibiting high plasma estrogen levels |
title_full_unstemmed |
Proteomic characterization of testicular protein expression in male hornyhead turbot exhibiting high plasma estrogen levels |
title_sort |
proteomic characterization of testicular protein expression in male hornyhead turbot exhibiting high plasma estrogen levels |
publisher |
California State University, Long Beach |
publishDate |
2014 |
url |
http://pqdtopen.proquest.com/#viewpdf?dispub=1569889 |
genre |
Turbot |
genre_facet |
Turbot |
op_relation |
http://pqdtopen.proquest.com/#viewpdf?dispub=1569889 |
_version_ |
1766230625890598912 |