Diversity and selection in the major histocompatibility complex: DQA and immune function in IRL and Atlantic bottlenose dolphines (Tursiops truncatus)
The Major Histocompatibility Complex (MHC) encodes proteins critical to the vertebrate immune response; therefore MHC diversity is an indicator of population health. I have (1) Isolated exon 2 of the class II gene DQA in Tursiops truncatus in the North Indian River Lagoon (IRL) (n=17), South IRL (n=...
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Florida Atlantic University
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ftproquest:oai:pqdtoai.proquest.com:1524502 2023-05-15T17:53:46+02:00 Diversity and selection in the major histocompatibility complex: DQA and immune function in IRL and Atlantic bottlenose dolphines (Tursiops truncatus) Ferrer, Tatiana 2013-01-01 00:00:01.0 http://pqdtopen.proquest.com/#viewpdf?dispub=1524502 ENG eng Florida Atlantic University http://pqdtopen.proquest.com/#viewpdf?dispub=1524502 Molecular biology|Genetics|Conservation thesis 2013 ftproquest 2021-03-13T17:36:00Z The Major Histocompatibility Complex (MHC) encodes proteins critical to the vertebrate immune response; therefore MHC diversity is an indicator of population health. I have (1) Isolated exon 2 of the class II gene DQA in Tursiops truncatus in the North Indian River Lagoon (IRL) (n=17), South IRL (n=29) and adjacent Atlantic waters (n=20), (2) assessed genetic variability between groups, (3) developed a method to genotype individuals, (4) typed 11 unique alleles in 66 individuals, (5) detected geographic patterns of diversity between estuarine and coastal individuals (FST=0.1255, p≤0.05), (6) found evidence of positive selection centered in the binding pockets P1, P6 and P9 of the peptide binding region (ω=2.08), (7) found that patterns of polymorphism did not closely match patterns of diversity in neutral markers, (8) performed a pilot study with Orcinus orca . The initial findings highlight the need for further comparative work and suggest that silent mutations are not neutral. Thesis Orca Orcinus orca PQDT Open: Open Access Dissertations and Theses (ProQuest) Indian |
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Open Polar |
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PQDT Open: Open Access Dissertations and Theses (ProQuest) |
op_collection_id |
ftproquest |
language |
English |
topic |
Molecular biology|Genetics|Conservation |
spellingShingle |
Molecular biology|Genetics|Conservation Ferrer, Tatiana Diversity and selection in the major histocompatibility complex: DQA and immune function in IRL and Atlantic bottlenose dolphines (Tursiops truncatus) |
topic_facet |
Molecular biology|Genetics|Conservation |
description |
The Major Histocompatibility Complex (MHC) encodes proteins critical to the vertebrate immune response; therefore MHC diversity is an indicator of population health. I have (1) Isolated exon 2 of the class II gene DQA in Tursiops truncatus in the North Indian River Lagoon (IRL) (n=17), South IRL (n=29) and adjacent Atlantic waters (n=20), (2) assessed genetic variability between groups, (3) developed a method to genotype individuals, (4) typed 11 unique alleles in 66 individuals, (5) detected geographic patterns of diversity between estuarine and coastal individuals (FST=0.1255, p≤0.05), (6) found evidence of positive selection centered in the binding pockets P1, P6 and P9 of the peptide binding region (ω=2.08), (7) found that patterns of polymorphism did not closely match patterns of diversity in neutral markers, (8) performed a pilot study with Orcinus orca . The initial findings highlight the need for further comparative work and suggest that silent mutations are not neutral. |
format |
Thesis |
author |
Ferrer, Tatiana |
author_facet |
Ferrer, Tatiana |
author_sort |
Ferrer, Tatiana |
title |
Diversity and selection in the major histocompatibility complex: DQA and immune function in IRL and Atlantic bottlenose dolphines (Tursiops truncatus) |
title_short |
Diversity and selection in the major histocompatibility complex: DQA and immune function in IRL and Atlantic bottlenose dolphines (Tursiops truncatus) |
title_full |
Diversity and selection in the major histocompatibility complex: DQA and immune function in IRL and Atlantic bottlenose dolphines (Tursiops truncatus) |
title_fullStr |
Diversity and selection in the major histocompatibility complex: DQA and immune function in IRL and Atlantic bottlenose dolphines (Tursiops truncatus) |
title_full_unstemmed |
Diversity and selection in the major histocompatibility complex: DQA and immune function in IRL and Atlantic bottlenose dolphines (Tursiops truncatus) |
title_sort |
diversity and selection in the major histocompatibility complex: dqa and immune function in irl and atlantic bottlenose dolphines (tursiops truncatus) |
publisher |
Florida Atlantic University |
publishDate |
2013 |
url |
http://pqdtopen.proquest.com/#viewpdf?dispub=1524502 |
geographic |
Indian |
geographic_facet |
Indian |
genre |
Orca Orcinus orca |
genre_facet |
Orca Orcinus orca |
op_relation |
http://pqdtopen.proquest.com/#viewpdf?dispub=1524502 |
_version_ |
1766161480924790784 |