Implementing a PCR-RFLP analysis method to identify illegal exotic oysters for the State of Texas
An internal validation study was conducted to consider the adoption of a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method shown in a study by Cordes et al (2008) to provide discrimination between commercially harvested oyster species. Through the digestion of ampl...
Main Author: | |
---|---|
Format: | Thesis |
Language: | English |
Published: |
University of North Texas Health Science Center at Fort Worth
2011
|
Subjects: | |
Online Access: | http://pqdtopen.proquest.com/#viewpdf?dispub=1497762 |
id |
ftproquest:oai:pqdtoai.proquest.com:1497762 |
---|---|
record_format |
openpolar |
spelling |
ftproquest:oai:pqdtoai.proquest.com:1497762 2023-05-15T15:58:31+02:00 Implementing a PCR-RFLP analysis method to identify illegal exotic oysters for the State of Texas Parham, Van Edward, III 2011-01-01 00:00:01.0 http://pqdtopen.proquest.com/#viewpdf?dispub=1497762 ENG eng University of North Texas Health Science Center at Fort Worth http://pqdtopen.proquest.com/#viewpdf?dispub=1497762 Genetics|Zoology|Criminology|Aquatic sciences thesis 2011 ftproquest 2021-03-13T17:35:26Z An internal validation study was conducted to consider the adoption of a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method shown in a study by Cordes et al (2008) to provide discrimination between commercially harvested oyster species. Through the digestion of amplified fragments of the ITS-1 nuclear DNA gene region and the COI mitochondrial DNA region with the restriction enzymes Hae III and Dde I respectively, a unique fragment profile is achieved that allows for a genetic method of differentiation between the two most prevalent edible oyster species that are marketed in Texas, the native, Crassostrea virginica and an exotic, Crassostrea gigas. The results of this study provide the Texas Parks and Wildlife Law Enforcement Forensic Laboratory with a genetic method to assist with the identification of wildlife code violations of possession of live C. gigas. Thesis Crassostrea gigas PQDT Open: Open Access Dissertations and Theses (ProQuest) |
institution |
Open Polar |
collection |
PQDT Open: Open Access Dissertations and Theses (ProQuest) |
op_collection_id |
ftproquest |
language |
English |
topic |
Genetics|Zoology|Criminology|Aquatic sciences |
spellingShingle |
Genetics|Zoology|Criminology|Aquatic sciences Parham, Van Edward, III Implementing a PCR-RFLP analysis method to identify illegal exotic oysters for the State of Texas |
topic_facet |
Genetics|Zoology|Criminology|Aquatic sciences |
description |
An internal validation study was conducted to consider the adoption of a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method shown in a study by Cordes et al (2008) to provide discrimination between commercially harvested oyster species. Through the digestion of amplified fragments of the ITS-1 nuclear DNA gene region and the COI mitochondrial DNA region with the restriction enzymes Hae III and Dde I respectively, a unique fragment profile is achieved that allows for a genetic method of differentiation between the two most prevalent edible oyster species that are marketed in Texas, the native, Crassostrea virginica and an exotic, Crassostrea gigas. The results of this study provide the Texas Parks and Wildlife Law Enforcement Forensic Laboratory with a genetic method to assist with the identification of wildlife code violations of possession of live C. gigas. |
format |
Thesis |
author |
Parham, Van Edward, III |
author_facet |
Parham, Van Edward, III |
author_sort |
Parham, Van Edward, III |
title |
Implementing a PCR-RFLP analysis method to identify illegal exotic oysters for the State of Texas |
title_short |
Implementing a PCR-RFLP analysis method to identify illegal exotic oysters for the State of Texas |
title_full |
Implementing a PCR-RFLP analysis method to identify illegal exotic oysters for the State of Texas |
title_fullStr |
Implementing a PCR-RFLP analysis method to identify illegal exotic oysters for the State of Texas |
title_full_unstemmed |
Implementing a PCR-RFLP analysis method to identify illegal exotic oysters for the State of Texas |
title_sort |
implementing a pcr-rflp analysis method to identify illegal exotic oysters for the state of texas |
publisher |
University of North Texas Health Science Center at Fort Worth |
publishDate |
2011 |
url |
http://pqdtopen.proquest.com/#viewpdf?dispub=1497762 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
http://pqdtopen.proquest.com/#viewpdf?dispub=1497762 |
_version_ |
1766394262853779456 |