CCAAT/Enhancer-Binding Protein Delta (C/EBP-delta) Expression in Antarctic Fishes: Implications for Cell Cycle and Apoptosis
Chapter 1: Antarctic fishes are extremely cold adapted. Despite their inability to upregulate heat shock proteins, recent studies have demonstrated a capacity for heat response in these animals. A cDNA microarray study looked at the Notothenioid fish Trematomus bernacchii and revealed heat sensitivi...
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ftportlandstate:oai:pdxscholar.library.pdx.edu:open_access_etds-1993 2023-06-11T04:05:18+02:00 CCAAT/Enhancer-Binding Protein Delta (C/EBP-delta) Expression in Antarctic Fishes: Implications for Cell Cycle and Apoptosis Sleadd, Isaac Martin 2013-08-13T07:00:00Z application/pdf https://pdxscholar.library.pdx.edu/open_access_etds/994 https://doi.org/10.15760/etd.994 https://pdxscholar.library.pdx.edu/context/open_access_etds/article/1993/viewcontent/Sleadd_psu_0180D_10897.pdf English eng PDXScholar https://pdxscholar.library.pdx.edu/open_access_etds/994 doi:10.15760/etd.994 https://pdxscholar.library.pdx.edu/context/open_access_etds/article/1993/viewcontent/Sleadd_psu_0180D_10897.pdf In Copyright. URI: http://rightsstatements.org/vocab/InC/1.0/ This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). Dissertations and Theses Fishes -- Effect of stress on -- Antarctic Ocean Climatic changes -- Antarctica DNA-protein interactions -- Research Marine animals -- Adaptation -- Polar regions Aquaculture and Fisheries Climate Marine Biology text 2013 ftportlandstate https://doi.org/10.15760/etd.994 2023-05-04T18:02:28Z Chapter 1: Antarctic fishes are extremely cold adapted. Despite their inability to upregulate heat shock proteins, recent studies have demonstrated a capacity for heat response in these animals. A cDNA microarray study looked at the Notothenioid fish Trematomus bernacchii and revealed heat sensitivities for hundreds of genes, two of which code for members of the CCAAT/Enhancer-binding protein (C/EBP) family of transcription factors. These molecular switches are best known for their roles in apoptosis, inflammation and cell cycle arrest. This dissertation further elucidates the role of C/EBP-delta in the Antarctic fishes T. bernacchii and Pagothenia borchgrevinki. Chapter 2: C/EBP-delta is constitutively expressed in unstressed, field-acclimated (ca. -1.86°C) animals in a highly tissue-specific manner. White muscle tissue contains the highest C/EBP-delta concentration, which is further increased in response to sublethal heat stress at 2.0 or 4.0°C. This response is mostly acute and transitory, but a lesser upregulation was observed in fishes held for one month at 4.0°C. Chapter 3: The heat-induced nuclear translocation of C/EBP-delta--as determined by immunohistochemistry--appears to be time, tissue and species specific with spleen, heart and retinae being particularly responsive in certain situations. Chapter 4: Protein concentrations of proliferating cell nuclear antigen are tissue specific and variably heat responsive. Surprisingly, levels appear to be positively correlated with C/EBP-delta. Chapter 5: Flow cytometry revealed increasingly high temperatures reduce the proportion of G1 cells while increasing the abundance of apoptotic cells. Chapter 6: These findings are discussed in the context of global climate change and the cellular stress response. Text Antarc* Antarctic Antarctic Ocean Antarctica Portland State University: PDXScholar Antarctic The Antarctic Antarctic Ocean |
institution |
Open Polar |
collection |
Portland State University: PDXScholar |
op_collection_id |
ftportlandstate |
language |
English |
topic |
Fishes -- Effect of stress on -- Antarctic Ocean Climatic changes -- Antarctica DNA-protein interactions -- Research Marine animals -- Adaptation -- Polar regions Aquaculture and Fisheries Climate Marine Biology |
spellingShingle |
Fishes -- Effect of stress on -- Antarctic Ocean Climatic changes -- Antarctica DNA-protein interactions -- Research Marine animals -- Adaptation -- Polar regions Aquaculture and Fisheries Climate Marine Biology Sleadd, Isaac Martin CCAAT/Enhancer-Binding Protein Delta (C/EBP-delta) Expression in Antarctic Fishes: Implications for Cell Cycle and Apoptosis |
topic_facet |
Fishes -- Effect of stress on -- Antarctic Ocean Climatic changes -- Antarctica DNA-protein interactions -- Research Marine animals -- Adaptation -- Polar regions Aquaculture and Fisheries Climate Marine Biology |
description |
Chapter 1: Antarctic fishes are extremely cold adapted. Despite their inability to upregulate heat shock proteins, recent studies have demonstrated a capacity for heat response in these animals. A cDNA microarray study looked at the Notothenioid fish Trematomus bernacchii and revealed heat sensitivities for hundreds of genes, two of which code for members of the CCAAT/Enhancer-binding protein (C/EBP) family of transcription factors. These molecular switches are best known for their roles in apoptosis, inflammation and cell cycle arrest. This dissertation further elucidates the role of C/EBP-delta in the Antarctic fishes T. bernacchii and Pagothenia borchgrevinki. Chapter 2: C/EBP-delta is constitutively expressed in unstressed, field-acclimated (ca. -1.86°C) animals in a highly tissue-specific manner. White muscle tissue contains the highest C/EBP-delta concentration, which is further increased in response to sublethal heat stress at 2.0 or 4.0°C. This response is mostly acute and transitory, but a lesser upregulation was observed in fishes held for one month at 4.0°C. Chapter 3: The heat-induced nuclear translocation of C/EBP-delta--as determined by immunohistochemistry--appears to be time, tissue and species specific with spleen, heart and retinae being particularly responsive in certain situations. Chapter 4: Protein concentrations of proliferating cell nuclear antigen are tissue specific and variably heat responsive. Surprisingly, levels appear to be positively correlated with C/EBP-delta. Chapter 5: Flow cytometry revealed increasingly high temperatures reduce the proportion of G1 cells while increasing the abundance of apoptotic cells. Chapter 6: These findings are discussed in the context of global climate change and the cellular stress response. |
format |
Text |
author |
Sleadd, Isaac Martin |
author_facet |
Sleadd, Isaac Martin |
author_sort |
Sleadd, Isaac Martin |
title |
CCAAT/Enhancer-Binding Protein Delta (C/EBP-delta) Expression in Antarctic Fishes: Implications for Cell Cycle and Apoptosis |
title_short |
CCAAT/Enhancer-Binding Protein Delta (C/EBP-delta) Expression in Antarctic Fishes: Implications for Cell Cycle and Apoptosis |
title_full |
CCAAT/Enhancer-Binding Protein Delta (C/EBP-delta) Expression in Antarctic Fishes: Implications for Cell Cycle and Apoptosis |
title_fullStr |
CCAAT/Enhancer-Binding Protein Delta (C/EBP-delta) Expression in Antarctic Fishes: Implications for Cell Cycle and Apoptosis |
title_full_unstemmed |
CCAAT/Enhancer-Binding Protein Delta (C/EBP-delta) Expression in Antarctic Fishes: Implications for Cell Cycle and Apoptosis |
title_sort |
ccaat/enhancer-binding protein delta (c/ebp-delta) expression in antarctic fishes: implications for cell cycle and apoptosis |
publisher |
PDXScholar |
publishDate |
2013 |
url |
https://pdxscholar.library.pdx.edu/open_access_etds/994 https://doi.org/10.15760/etd.994 https://pdxscholar.library.pdx.edu/context/open_access_etds/article/1993/viewcontent/Sleadd_psu_0180D_10897.pdf |
geographic |
Antarctic The Antarctic Antarctic Ocean |
geographic_facet |
Antarctic The Antarctic Antarctic Ocean |
genre |
Antarc* Antarctic Antarctic Ocean Antarctica |
genre_facet |
Antarc* Antarctic Antarctic Ocean Antarctica |
op_source |
Dissertations and Theses |
op_relation |
https://pdxscholar.library.pdx.edu/open_access_etds/994 doi:10.15760/etd.994 https://pdxscholar.library.pdx.edu/context/open_access_etds/article/1993/viewcontent/Sleadd_psu_0180D_10897.pdf |
op_rights |
In Copyright. URI: http://rightsstatements.org/vocab/InC/1.0/ This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). |
op_doi |
https://doi.org/10.15760/etd.994 |
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1768374056747270144 |