Expression of Growth Arrest and DNA Damage Protein 45-alpha (gadd45-alpha) and the CCAAT/enhancer binding protein-delta (C/EBP-delta) in Fishes Exposed to Heat and Hypoxia
The cellular stress response (CSR) is one of the most highly conserved mechanisms among all organisms. Cellular stress can be defined as damage or the threat of damage to proteins, macromolecules and/or DNA. The response to damage can involve cell cycle regulation, protein chaperoning, DNA repair or...
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ftportlandstate:oai:pdxscholar.library.pdx.edu:open_access_etds-1942 2023-05-15T13:58:46+02:00 Expression of Growth Arrest and DNA Damage Protein 45-alpha (gadd45-alpha) and the CCAAT/enhancer binding protein-delta (C/EBP-delta) in Fishes Exposed to Heat and Hypoxia Hassumani, Daniel Omar 2013-03-20T07:00:00Z application/pdf https://pdxscholar.library.pdx.edu/open_access_etds/943 https://pdxscholar.library.pdx.edu/cgi/viewcontent.cgi?article=1942&context=open_access_etds English eng PDXScholar https://pdxscholar.library.pdx.edu/open_access_etds/943 https://pdxscholar.library.pdx.edu/cgi/viewcontent.cgi?article=1942&context=open_access_etds In Copyright. URI: http://rightsstatements.org/vocab/InC/1.0/ This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). Dissertations and Theses Cell physiology Cellular control mechanisms Cell cycle Stress (Physiology) Cell Biology text 2013 ftportlandstate 2022-01-09T19:23:14Z The cellular stress response (CSR) is one of the most highly conserved mechanisms among all organisms. Cellular stress can be defined as damage or the threat of damage to proteins, macromolecules and/or DNA. The response to damage can involve cell cycle regulation, protein chaperoning, DNA repair or, if macromolecular damage is too severe, apoptotic mechanisms can be initiated. This thesis details experiments that were designed to examine the cellular response to non-lethal environmental stressors at the protein level, using two fish species as study models. Two proteins that can cause cell cycle arrest and apoptosis mechanisms were examined. Expression of the CCAAT enhancer binding protein-delta (C/EBP-[delta]) was examined in the zebrafish, Danio rerio, exposed to acute, non-lethal hypoxic conditions. While C/EBP-[delta] was expressed constitutively in control individuals during all time points, exposure to hypoxic conditions did not have a consistent significant effect on C/EBP-[delta] expression (two-way ANOVA, P>0.05) in zebrafish white muscle tissue. In a second study, the expression of the growth arrest and DNA damage 45-alpha protein (gadd45-[alpha], a mediator of cell cycle arrest and perhaps apoptosis was examined in heat-stressed liver tissue of an extremely cold-adapted Antarctic fish, Trematomus bernacchii. Gadd45-[alpha] levels were higher in fish exposure to 2°C across all time points (one-way ANOVA; P Text Antarc* Antarctic Portland State University: PDXScholar Antarctic |
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Open Polar |
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Portland State University: PDXScholar |
op_collection_id |
ftportlandstate |
language |
English |
topic |
Cell physiology Cellular control mechanisms Cell cycle Stress (Physiology) Cell Biology |
spellingShingle |
Cell physiology Cellular control mechanisms Cell cycle Stress (Physiology) Cell Biology Hassumani, Daniel Omar Expression of Growth Arrest and DNA Damage Protein 45-alpha (gadd45-alpha) and the CCAAT/enhancer binding protein-delta (C/EBP-delta) in Fishes Exposed to Heat and Hypoxia |
topic_facet |
Cell physiology Cellular control mechanisms Cell cycle Stress (Physiology) Cell Biology |
description |
The cellular stress response (CSR) is one of the most highly conserved mechanisms among all organisms. Cellular stress can be defined as damage or the threat of damage to proteins, macromolecules and/or DNA. The response to damage can involve cell cycle regulation, protein chaperoning, DNA repair or, if macromolecular damage is too severe, apoptotic mechanisms can be initiated. This thesis details experiments that were designed to examine the cellular response to non-lethal environmental stressors at the protein level, using two fish species as study models. Two proteins that can cause cell cycle arrest and apoptosis mechanisms were examined. Expression of the CCAAT enhancer binding protein-delta (C/EBP-[delta]) was examined in the zebrafish, Danio rerio, exposed to acute, non-lethal hypoxic conditions. While C/EBP-[delta] was expressed constitutively in control individuals during all time points, exposure to hypoxic conditions did not have a consistent significant effect on C/EBP-[delta] expression (two-way ANOVA, P>0.05) in zebrafish white muscle tissue. In a second study, the expression of the growth arrest and DNA damage 45-alpha protein (gadd45-[alpha], a mediator of cell cycle arrest and perhaps apoptosis was examined in heat-stressed liver tissue of an extremely cold-adapted Antarctic fish, Trematomus bernacchii. Gadd45-[alpha] levels were higher in fish exposure to 2°C across all time points (one-way ANOVA; P |
format |
Text |
author |
Hassumani, Daniel Omar |
author_facet |
Hassumani, Daniel Omar |
author_sort |
Hassumani, Daniel Omar |
title |
Expression of Growth Arrest and DNA Damage Protein 45-alpha (gadd45-alpha) and the CCAAT/enhancer binding protein-delta (C/EBP-delta) in Fishes Exposed to Heat and Hypoxia |
title_short |
Expression of Growth Arrest and DNA Damage Protein 45-alpha (gadd45-alpha) and the CCAAT/enhancer binding protein-delta (C/EBP-delta) in Fishes Exposed to Heat and Hypoxia |
title_full |
Expression of Growth Arrest and DNA Damage Protein 45-alpha (gadd45-alpha) and the CCAAT/enhancer binding protein-delta (C/EBP-delta) in Fishes Exposed to Heat and Hypoxia |
title_fullStr |
Expression of Growth Arrest and DNA Damage Protein 45-alpha (gadd45-alpha) and the CCAAT/enhancer binding protein-delta (C/EBP-delta) in Fishes Exposed to Heat and Hypoxia |
title_full_unstemmed |
Expression of Growth Arrest and DNA Damage Protein 45-alpha (gadd45-alpha) and the CCAAT/enhancer binding protein-delta (C/EBP-delta) in Fishes Exposed to Heat and Hypoxia |
title_sort |
expression of growth arrest and dna damage protein 45-alpha (gadd45-alpha) and the ccaat/enhancer binding protein-delta (c/ebp-delta) in fishes exposed to heat and hypoxia |
publisher |
PDXScholar |
publishDate |
2013 |
url |
https://pdxscholar.library.pdx.edu/open_access_etds/943 https://pdxscholar.library.pdx.edu/cgi/viewcontent.cgi?article=1942&context=open_access_etds |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Dissertations and Theses |
op_relation |
https://pdxscholar.library.pdx.edu/open_access_etds/943 https://pdxscholar.library.pdx.edu/cgi/viewcontent.cgi?article=1942&context=open_access_etds |
op_rights |
In Copyright. URI: http://rightsstatements.org/vocab/InC/1.0/ This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). |
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