Assessment of astaxanthin accumulation in hepatocytes of Atlantic salmon fed different diets using NMR spectroscopy
This study aimed to assess the astaxanthin (Ax) accumulation in hepatocytes isolated from farmed Atlantic salmon fed different diets (rich marine, poor, poor with marine phospholipids (MPL) and poor with docosahexaenoic acid (DHA)). Nuclear magnetic resonance (NMR) spectroscopy was used for the Ax d...
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Online Access: | http://hdl.handle.net/11589/210579 https://doi.org/10.3390/molecules25081769 |
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ftpolitecnicbari:oai:iris.poliba.it:11589/210579 2024-04-14T08:09:02+00:00 Assessment of astaxanthin accumulation in hepatocytes of Atlantic salmon fed different diets using NMR spectroscopy Elena Shumilina Alessandra Ciampa Trine Ytrestøyl Alexander Dikiy Shumilina, Elena Ciampa, Alessandra Ytrestøyl, Trine Dikiy, Alexander 2020 ELETTRONICO http://hdl.handle.net/11589/210579 https://doi.org/10.3390/molecules25081769 eng eng info:eu-repo/semantics/altIdentifier/wos/WOS:000534617300006 volume:25 issue:8 journal:MOLECULES http://hdl.handle.net/11589/210579 doi:10.3390/molecules25081769 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85083416646 info:eu-repo/semantics/openAccess Astaxanthin Atlantic salmon Carotenoid DHA Fish diet Fish feed Hepatocyte NMR Plant proteins info:eu-repo/semantics/article 2020 ftpolitecnicbari https://doi.org/10.3390/molecules25081769 2024-03-21T17:17:06Z This study aimed to assess the astaxanthin (Ax) accumulation in hepatocytes isolated from farmed Atlantic salmon fed different diets (rich marine, poor, poor with marine phospholipids (MPL) and poor with docosahexaenoic acid (DHA)). Nuclear magnetic resonance (NMR) spectroscopy was used for the Ax detection and quantification. The use of the 13C-enriched Ax allowed the assessment of short-time Ax metabolism. The substitution of fish oil and meal in fish feed on plant analogs and the addition of MPL caused further catabolism and decrease of Ax accumulation in hepatocytes from 17 to about 6 mg/kg or to almost zero in the case of DHA addition. Signals assignment of the native and 13C-enriched astaxanthin in acetone were performed using 1D and 2D NMR spectra. Article in Journal/Newspaper Atlantic salmon Politecnico di Bari: Iris POLIBA (Catalogo dei prodotti della Ricerca) Molecules 25 8 1769 |
institution |
Open Polar |
collection |
Politecnico di Bari: Iris POLIBA (Catalogo dei prodotti della Ricerca) |
op_collection_id |
ftpolitecnicbari |
language |
English |
topic |
Astaxanthin Atlantic salmon Carotenoid DHA Fish diet Fish feed Hepatocyte NMR Plant proteins |
spellingShingle |
Astaxanthin Atlantic salmon Carotenoid DHA Fish diet Fish feed Hepatocyte NMR Plant proteins Elena Shumilina Alessandra Ciampa Trine Ytrestøyl Alexander Dikiy Assessment of astaxanthin accumulation in hepatocytes of Atlantic salmon fed different diets using NMR spectroscopy |
topic_facet |
Astaxanthin Atlantic salmon Carotenoid DHA Fish diet Fish feed Hepatocyte NMR Plant proteins |
description |
This study aimed to assess the astaxanthin (Ax) accumulation in hepatocytes isolated from farmed Atlantic salmon fed different diets (rich marine, poor, poor with marine phospholipids (MPL) and poor with docosahexaenoic acid (DHA)). Nuclear magnetic resonance (NMR) spectroscopy was used for the Ax detection and quantification. The use of the 13C-enriched Ax allowed the assessment of short-time Ax metabolism. The substitution of fish oil and meal in fish feed on plant analogs and the addition of MPL caused further catabolism and decrease of Ax accumulation in hepatocytes from 17 to about 6 mg/kg or to almost zero in the case of DHA addition. Signals assignment of the native and 13C-enriched astaxanthin in acetone were performed using 1D and 2D NMR spectra. |
author2 |
Shumilina, Elena Ciampa, Alessandra Ytrestøyl, Trine Dikiy, Alexander |
format |
Article in Journal/Newspaper |
author |
Elena Shumilina Alessandra Ciampa Trine Ytrestøyl Alexander Dikiy |
author_facet |
Elena Shumilina Alessandra Ciampa Trine Ytrestøyl Alexander Dikiy |
author_sort |
Elena Shumilina |
title |
Assessment of astaxanthin accumulation in hepatocytes of Atlantic salmon fed different diets using NMR spectroscopy |
title_short |
Assessment of astaxanthin accumulation in hepatocytes of Atlantic salmon fed different diets using NMR spectroscopy |
title_full |
Assessment of astaxanthin accumulation in hepatocytes of Atlantic salmon fed different diets using NMR spectroscopy |
title_fullStr |
Assessment of astaxanthin accumulation in hepatocytes of Atlantic salmon fed different diets using NMR spectroscopy |
title_full_unstemmed |
Assessment of astaxanthin accumulation in hepatocytes of Atlantic salmon fed different diets using NMR spectroscopy |
title_sort |
assessment of astaxanthin accumulation in hepatocytes of atlantic salmon fed different diets using nmr spectroscopy |
publishDate |
2020 |
url |
http://hdl.handle.net/11589/210579 https://doi.org/10.3390/molecules25081769 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000534617300006 volume:25 issue:8 journal:MOLECULES http://hdl.handle.net/11589/210579 doi:10.3390/molecules25081769 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85083416646 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.3390/molecules25081769 |
container_title |
Molecules |
container_volume |
25 |
container_issue |
8 |
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1769 |
_version_ |
1796306489356320768 |