Transcriptional responses of Trichodesmium to natural inverse gradients of Fe and P availability
The filamentous diazotrophic cyanobacterium Trichodesmium is responsible for a significant fraction of marine di-nitrogen (N2) fixation. Growth and distribution of Trichodesmium and other diazotrophs in the vast oligotrophic subtropical gyres is influenced by iron (Fe) and phosphorus (P) availabilit...
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ftplymouthml:oai:plymsea.ac.uk:9469 2023-05-15T17:33:51+02:00 Transcriptional responses of Trichodesmium to natural inverse gradients of Fe and P availability Cerdan-Garcia, E Baylay, A Polyviou, D Woodward, EMS Wrightson, L Mahaffey, C Lohan, MC Moore, CM Bibby, T.S Robidart, J.C 2021-11-01 text http://plymsea.ac.uk/id/eprint/9469/ http://plymsea.ac.uk/id/eprint/9469/1/Cerdan-Garcia_2021%20Published.pdf https://doi.org/10.1038/s41396-021-01151-1 en eng Springer Nature http://plymsea.ac.uk/id/eprint/9469/1/Cerdan-Garcia_2021%20Published.pdf Cerdan-Garcia, E; Baylay, A; Polyviou, D; Woodward, EMS; Wrightson, L; Mahaffey, C; Lohan, MC; Moore, CM; Bibby, T.S; Robidart, J.C. 2021 Transcriptional responses of Trichodesmium to natural inverse gradients of Fe and P availability. ISME Journal - Multidisciplinary Journal of Microbial Ecology. https://doi.org/10.1038/s41396-021-01151-1 <https://doi.org/10.1038/s41396-021-01151-1> cc_by_4 CC-BY Biology Chemistry Marine Sciences Publication - Article PeerReviewed 2021 ftplymouthml https://doi.org/10.1038/s41396-021-01151-1 2022-09-13T05:49:58Z The filamentous diazotrophic cyanobacterium Trichodesmium is responsible for a significant fraction of marine di-nitrogen (N2) fixation. Growth and distribution of Trichodesmium and other diazotrophs in the vast oligotrophic subtropical gyres is influenced by iron (Fe) and phosphorus (P) availability, while reciprocally influencing the biogeochemistry of these nutrients. Here we use observations across natural inverse gradients in Fe and P in the North Atlantic subtropical gyre (NASG) to demonstrate how Trichodesmium acclimates in situ to resource availability. Transcriptomic analysis identified progressive upregulation of known ironstress biomarker genes with decreasing Fe availability, and progressive upregulation of genes involved in the acquisition of diverse P sources with decreasing P availability, while genes involved in N2 fixation were upregulated at the intersection under moderate Fe and P availability. Enhanced N2 fixation within the Fe and P co-stressed transition region was also associated with a distinct, consistent metabolic profile, including the expression of alternative photosynthetic pathways that potentially facilitate ATP generation required for N2 fixation with reduced net oxygen production. The observed response of Trichodesmium to availability of both Fe and P supports suggestions that these biogeochemically significant organisms employ unique molecular, and thus physiological responses as adaptations to specifically exploit the Fe and P co-limited niche they construct. Article in Journal/Newspaper North Atlantic Plymouth Marine Science Electronic Archive (PlyMSEA - Plymouth Marine Laboratory, PML) The ISME Journal 16 4 1055 1064 |
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Open Polar |
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Plymouth Marine Science Electronic Archive (PlyMSEA - Plymouth Marine Laboratory, PML) |
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ftplymouthml |
language |
English |
topic |
Biology Chemistry Marine Sciences |
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Biology Chemistry Marine Sciences Cerdan-Garcia, E Baylay, A Polyviou, D Woodward, EMS Wrightson, L Mahaffey, C Lohan, MC Moore, CM Bibby, T.S Robidart, J.C Transcriptional responses of Trichodesmium to natural inverse gradients of Fe and P availability |
topic_facet |
Biology Chemistry Marine Sciences |
description |
The filamentous diazotrophic cyanobacterium Trichodesmium is responsible for a significant fraction of marine di-nitrogen (N2) fixation. Growth and distribution of Trichodesmium and other diazotrophs in the vast oligotrophic subtropical gyres is influenced by iron (Fe) and phosphorus (P) availability, while reciprocally influencing the biogeochemistry of these nutrients. Here we use observations across natural inverse gradients in Fe and P in the North Atlantic subtropical gyre (NASG) to demonstrate how Trichodesmium acclimates in situ to resource availability. Transcriptomic analysis identified progressive upregulation of known ironstress biomarker genes with decreasing Fe availability, and progressive upregulation of genes involved in the acquisition of diverse P sources with decreasing P availability, while genes involved in N2 fixation were upregulated at the intersection under moderate Fe and P availability. Enhanced N2 fixation within the Fe and P co-stressed transition region was also associated with a distinct, consistent metabolic profile, including the expression of alternative photosynthetic pathways that potentially facilitate ATP generation required for N2 fixation with reduced net oxygen production. The observed response of Trichodesmium to availability of both Fe and P supports suggestions that these biogeochemically significant organisms employ unique molecular, and thus physiological responses as adaptations to specifically exploit the Fe and P co-limited niche they construct. |
format |
Article in Journal/Newspaper |
author |
Cerdan-Garcia, E Baylay, A Polyviou, D Woodward, EMS Wrightson, L Mahaffey, C Lohan, MC Moore, CM Bibby, T.S Robidart, J.C |
author_facet |
Cerdan-Garcia, E Baylay, A Polyviou, D Woodward, EMS Wrightson, L Mahaffey, C Lohan, MC Moore, CM Bibby, T.S Robidart, J.C |
author_sort |
Cerdan-Garcia, E |
title |
Transcriptional responses of Trichodesmium to natural inverse gradients of Fe and P availability |
title_short |
Transcriptional responses of Trichodesmium to natural inverse gradients of Fe and P availability |
title_full |
Transcriptional responses of Trichodesmium to natural inverse gradients of Fe and P availability |
title_fullStr |
Transcriptional responses of Trichodesmium to natural inverse gradients of Fe and P availability |
title_full_unstemmed |
Transcriptional responses of Trichodesmium to natural inverse gradients of Fe and P availability |
title_sort |
transcriptional responses of trichodesmium to natural inverse gradients of fe and p availability |
publisher |
Springer Nature |
publishDate |
2021 |
url |
http://plymsea.ac.uk/id/eprint/9469/ http://plymsea.ac.uk/id/eprint/9469/1/Cerdan-Garcia_2021%20Published.pdf https://doi.org/10.1038/s41396-021-01151-1 |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_relation |
http://plymsea.ac.uk/id/eprint/9469/1/Cerdan-Garcia_2021%20Published.pdf Cerdan-Garcia, E; Baylay, A; Polyviou, D; Woodward, EMS; Wrightson, L; Mahaffey, C; Lohan, MC; Moore, CM; Bibby, T.S; Robidart, J.C. 2021 Transcriptional responses of Trichodesmium to natural inverse gradients of Fe and P availability. ISME Journal - Multidisciplinary Journal of Microbial Ecology. https://doi.org/10.1038/s41396-021-01151-1 <https://doi.org/10.1038/s41396-021-01151-1> |
op_rights |
cc_by_4 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1038/s41396-021-01151-1 |
container_title |
The ISME Journal |
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16 |
container_issue |
4 |
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1055 |
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1064 |
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1766132489101770752 |