Performance of SNP markers for parentage analysis in the Italian Alpine brown bear using non-invasive samples
Determination of parentage provides valuable information for the conservation of wild populations, for instance, by allowing the monitoring of breeding success and inbreeding. Between 1999 and 2002, nine brown bears (Ursus arctos) were translocated to augment the remnant population of a few survivin...
Published in: | Nature Conservation |
---|---|
Main Authors: | , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Pensoft Publishers
2023
|
Subjects: | |
Online Access: | https://doi.org/10.3897/natureconservation.53.86739 https://natureconservation.pensoft.net/article/86739/ |
id |
ftpensoft:10.3897/natureconservation.53.86739 |
---|---|
record_format |
openpolar |
spelling |
ftpensoft:10.3897/natureconservation.53.86739 2023-09-05T13:23:53+02:00 Performance of SNP markers for parentage analysis in the Italian Alpine brown bear using non-invasive samples Giangregorio,Patrizia Mucci,Nadia Norman,Anita J. Pedrotti,Luca Filacorda,Stefano Molinari,Paolo Spong,Göran Davoli,Francesca 2023 text/html https://doi.org/10.3897/natureconservation.53.86739 https://natureconservation.pensoft.net/article/86739/ en eng Pensoft Publishers info:eu-repo/semantics/altIdentifier/eissn/1314-3301 info:eu-repo/semantics/altIdentifier/pissn/1314-6947 info:eu-repo/semantics/openAccess CC BY 4.0 Nature Conservation 53: 105-123 Colony FRANz markers combination microsatellites monitoring Ursus arctos arctos Research Article 2023 ftpensoft https://doi.org/10.3897/natureconservation.53.86739 2023-08-15T00:05:31Z Determination of parentage provides valuable information for the conservation of wild populations, for instance, by allowing the monitoring of breeding success and inbreeding. Between 1999 and 2002, nine brown bears (Ursus arctos) were translocated to augment the remnant population of a few surviving individuals in the Italian Alps, but only part of them reproduced, with a higher inbreeding risk occurrence in the long-time. Currently, in the Alpine population, parentage tests are assessed through the analysis of 15 microsatellite loci (STRs), but the reduction of genetic variability in future generations will need the use of additional informative markers. Single nucleotide polymorphisms (SNPs) have been proven to be useful and reliable in individual identification and family reconstruction; moreover, they can perform well on low-quality samples. In this study, we analysed 51 SNPs to generate a SNP multilocus genotype dataset of 54 Alpine brown bears (Ursus arctos) and compared its performance in parentage analysis with the validated STR dataset. We found that SNPs alone are not sufficient to determine parentage relationships, but the combination of SNPs and STRs provided unambiguous parentage assignments. The combined panel also performed better than STRs when true parents were not present in the dataset and, consequently, showed higher values of assignment probabilities. Article in Journal/Newspaper Ursus arctos Pensoft Publishers Nature Conservation 53 105 123 |
institution |
Open Polar |
collection |
Pensoft Publishers |
op_collection_id |
ftpensoft |
language |
English |
topic |
Colony FRANz markers combination microsatellites monitoring Ursus arctos arctos |
spellingShingle |
Colony FRANz markers combination microsatellites monitoring Ursus arctos arctos Giangregorio,Patrizia Mucci,Nadia Norman,Anita J. Pedrotti,Luca Filacorda,Stefano Molinari,Paolo Spong,Göran Davoli,Francesca Performance of SNP markers for parentage analysis in the Italian Alpine brown bear using non-invasive samples |
topic_facet |
Colony FRANz markers combination microsatellites monitoring Ursus arctos arctos |
description |
Determination of parentage provides valuable information for the conservation of wild populations, for instance, by allowing the monitoring of breeding success and inbreeding. Between 1999 and 2002, nine brown bears (Ursus arctos) were translocated to augment the remnant population of a few surviving individuals in the Italian Alps, but only part of them reproduced, with a higher inbreeding risk occurrence in the long-time. Currently, in the Alpine population, parentage tests are assessed through the analysis of 15 microsatellite loci (STRs), but the reduction of genetic variability in future generations will need the use of additional informative markers. Single nucleotide polymorphisms (SNPs) have been proven to be useful and reliable in individual identification and family reconstruction; moreover, they can perform well on low-quality samples. In this study, we analysed 51 SNPs to generate a SNP multilocus genotype dataset of 54 Alpine brown bears (Ursus arctos) and compared its performance in parentage analysis with the validated STR dataset. We found that SNPs alone are not sufficient to determine parentage relationships, but the combination of SNPs and STRs provided unambiguous parentage assignments. The combined panel also performed better than STRs when true parents were not present in the dataset and, consequently, showed higher values of assignment probabilities. |
format |
Article in Journal/Newspaper |
author |
Giangregorio,Patrizia Mucci,Nadia Norman,Anita J. Pedrotti,Luca Filacorda,Stefano Molinari,Paolo Spong,Göran Davoli,Francesca |
author_facet |
Giangregorio,Patrizia Mucci,Nadia Norman,Anita J. Pedrotti,Luca Filacorda,Stefano Molinari,Paolo Spong,Göran Davoli,Francesca |
author_sort |
Giangregorio,Patrizia |
title |
Performance of SNP markers for parentage analysis in the Italian Alpine brown bear using non-invasive samples |
title_short |
Performance of SNP markers for parentage analysis in the Italian Alpine brown bear using non-invasive samples |
title_full |
Performance of SNP markers for parentage analysis in the Italian Alpine brown bear using non-invasive samples |
title_fullStr |
Performance of SNP markers for parentage analysis in the Italian Alpine brown bear using non-invasive samples |
title_full_unstemmed |
Performance of SNP markers for parentage analysis in the Italian Alpine brown bear using non-invasive samples |
title_sort |
performance of snp markers for parentage analysis in the italian alpine brown bear using non-invasive samples |
publisher |
Pensoft Publishers |
publishDate |
2023 |
url |
https://doi.org/10.3897/natureconservation.53.86739 https://natureconservation.pensoft.net/article/86739/ |
genre |
Ursus arctos |
genre_facet |
Ursus arctos |
op_source |
Nature Conservation 53: 105-123 |
op_relation |
info:eu-repo/semantics/altIdentifier/eissn/1314-3301 info:eu-repo/semantics/altIdentifier/pissn/1314-6947 |
op_rights |
info:eu-repo/semantics/openAccess CC BY 4.0 |
op_doi |
https://doi.org/10.3897/natureconservation.53.86739 |
container_title |
Nature Conservation |
container_volume |
53 |
container_start_page |
105 |
op_container_end_page |
123 |
_version_ |
1776204447996706816 |