Geometric morphometric methods for identification of oyster species based on morphology

• Main Authors: Liu,Qian, Guo,Yuepeng, Yang,Yanzhuo, Mao,Junxia, Wang,Xubo, Tian,Ying, Hao,Zhenlin
• Format: Article in Journal/Newspaper
• Language: English
• Published: Pensoft Publishers 2023
• Subjects: traditional morphometrics; geometric morphometrics; Pacific oyster; Pacific; Crassostrea gigas
• Online Access: https://doi.org/10.3897/arphapreprints.e116045; https://preprints.arphahub.com/article/116045/

Both genetic and environmental factors affect the morphology of oysters. Molecular identification is currently the primary means of species identification, but it is inconvenient and costly. In this study, we assessed the ability of geometric morphometric (GM) methods to identify two species of oysters (Crassostrea gigas and C. ariakensis). We used traditional morphometric and GM methods, including principal component analysis (PCA), thin-plate spline analysis (TPS), and canonical variable analysis (CVA), to identify specific features that distinguish the two species. We found that differences in shape can be visualized using GM methods. The Procrustes analysis revealed significant differences in shell morphology between C. gigas and C. ariakensis. The shells of C. ariakensis are more prominent at the widest point and are more scattered and have a greater variety of shapes. The shells of C. gigas are more oval in shape. PCA results indicated that PC1 explained 45.22%, PC2 explained 22.09%, and PC3 explained 10.98% of the variation between the two populations, which suggests that the main morphological differences are concentrated in these three principal components. Combining the TPS analysis function plots showed that the shell shape of C. ariakensis is mainly elongated and spindle-shaped, whereas the shell shape of C. gigas is more oval. The CVA results showed that the classification rate for the two populations reached 100% which means that C. ariakensis and C. gigas have distinct differences in shell morphology and can be completely separated based on morphological characteristics. Through these methods, a more comprehensive understanding of the morphological characteristics of different oyster populations can be obtained, providing a reference for oyster classification and identification.