Challenges in building barcode reference libraries for marine invertebrates exemplified by the genus Prionospio (Annelida: Spionidae)

• Published in: ARPHA Conference Abstracts
• Main Authors: Hektoen,Martin, Ekrem,Torbjørn, Bakken,Torkild
• Format: Conference Object
• Language: English
• Published: Pensoft Publishers 2021
• Subjects: Reference libraries; Unfit primers; Polychaeta; Spionidae; Carr; Folmer; Malmgren; North East Atlantic
• Online Access: https://doi.org/10.3897/aca.4.e64799; https://aca.pensoft.net/article/64799/

The completeness of reference libraries is often a limiting factor in the effectiveness of biomonitoring using molecular tools. The fact that these libraries are often built upon Sanger sequencing can create a substantial bias due to poor primer fits and unoptimized lab protocols. Some taxa of marin...