Karyotype and COI gene sequence of Chironomus heteropilicornis Wülker, 1996 (Diptera, Chironomidae) from the Gydan Peninsula, Russia
The karyotype features and gene COI sequence of Chironomus heteropilicornis Wülker, 1996 from the Gydan Peninsula are presented for the first time. Nine banding sequences were determined, eight of them hpiA2, hpiB1, hpiC1, hpiC2, hpiD1, hpiE1, hpiF3 and hpiG1 were previously known from European, Geo...
Published in: | Comparative Cytogenetics |
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Online Access: | https://doi.org/10.3897/CompCytogen.v15i4.73135 https://compcytogen.pensoft.net/article/73135/ |
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ftpensoft:10.3897/CompCytogen.v15i4.73135 2023-05-15T16:29:40+02:00 Karyotype and COI gene sequence of Chironomus heteropilicornis Wülker, 1996 (Diptera, Chironomidae) from the Gydan Peninsula, Russia Bolshakov,Viktor Prokin,Alexander Artemenko,Sergey 2021 text/html https://doi.org/10.3897/CompCytogen.v15i4.73135 https://compcytogen.pensoft.net/article/73135/ en eng Pensoft Publishers info:eu-repo/semantics/altIdentifier/eissn/1993-078X info:eu-repo/semantics/altIdentifier/pissn/1993-0771 info:eu-repo/semantics/openAccess Comparative Cytogenetics 15(4): 447-458 Chironomidae Chironomus heteropilicornis COI Diptera DNA-barcode Gydan Peninsula karyotype Research Article 2021 ftpensoft https://doi.org/10.3897/CompCytogen.v15i4.73135 2022-03-01T12:49:33Z The karyotype features and gene COI sequence of Chironomus heteropilicornis Wülker, 1996 from the Gydan Peninsula are presented for the first time. Nine banding sequences were determined, eight of them hpiA2, hpiB1, hpiC1, hpiC2, hpiD1, hpiE1, hpiF3 and hpiG1 were previously known from European, Georgian (South Caucasus) and Siberian populations. One new banding sequence for Ch. heteropilicornis, hpiB2, was found. The hpiA2 banding sequence was found in all individuals, and this is its second finding after the Georgian population (Karmokov 2019). The hpiF3 banding sequence was found only in the homozygous state. Additional B-chromosomes are absent. The genetic distances (K2P) between Ch. heteropilicornis COI gene sequence from Gydan Peninsula and Norway are 1.1–1.3%, and Georgia – 1.8%, much lower than the commonly accepted threshold of 3% for species of genus Chironomus Meigen, 1803. The phylogenetic tree for COI gene sequences estimated by Bayesian inference showed geographically determined clusters of Norway and Gydan and a separate lineage of the Georgian population of Ch. heteropilicornis. The analysis of karyotype and COI gene sequences shows that the population of Ch. heteropilicornis from the Gydan Peninsula has an intermediate position within the Ch. pilicornis group between Georgian, Yakutian and Norwegian populations. The position of Ch. pilicornis Fabricius, 1787 from Canada and Greenland on the phylogenetic tree is discussed. Article in Journal/Newspaper Greenland Pensoft Publishers Canada Greenland Norway Comparative Cytogenetics 15 4 447 458 |
institution |
Open Polar |
collection |
Pensoft Publishers |
op_collection_id |
ftpensoft |
language |
English |
topic |
Chironomidae Chironomus heteropilicornis COI Diptera DNA-barcode Gydan Peninsula karyotype |
spellingShingle |
Chironomidae Chironomus heteropilicornis COI Diptera DNA-barcode Gydan Peninsula karyotype Bolshakov,Viktor Prokin,Alexander Artemenko,Sergey Karyotype and COI gene sequence of Chironomus heteropilicornis Wülker, 1996 (Diptera, Chironomidae) from the Gydan Peninsula, Russia |
topic_facet |
Chironomidae Chironomus heteropilicornis COI Diptera DNA-barcode Gydan Peninsula karyotype |
description |
The karyotype features and gene COI sequence of Chironomus heteropilicornis Wülker, 1996 from the Gydan Peninsula are presented for the first time. Nine banding sequences were determined, eight of them hpiA2, hpiB1, hpiC1, hpiC2, hpiD1, hpiE1, hpiF3 and hpiG1 were previously known from European, Georgian (South Caucasus) and Siberian populations. One new banding sequence for Ch. heteropilicornis, hpiB2, was found. The hpiA2 banding sequence was found in all individuals, and this is its second finding after the Georgian population (Karmokov 2019). The hpiF3 banding sequence was found only in the homozygous state. Additional B-chromosomes are absent. The genetic distances (K2P) between Ch. heteropilicornis COI gene sequence from Gydan Peninsula and Norway are 1.1–1.3%, and Georgia – 1.8%, much lower than the commonly accepted threshold of 3% for species of genus Chironomus Meigen, 1803. The phylogenetic tree for COI gene sequences estimated by Bayesian inference showed geographically determined clusters of Norway and Gydan and a separate lineage of the Georgian population of Ch. heteropilicornis. The analysis of karyotype and COI gene sequences shows that the population of Ch. heteropilicornis from the Gydan Peninsula has an intermediate position within the Ch. pilicornis group between Georgian, Yakutian and Norwegian populations. The position of Ch. pilicornis Fabricius, 1787 from Canada and Greenland on the phylogenetic tree is discussed. |
format |
Article in Journal/Newspaper |
author |
Bolshakov,Viktor Prokin,Alexander Artemenko,Sergey |
author_facet |
Bolshakov,Viktor Prokin,Alexander Artemenko,Sergey |
author_sort |
Bolshakov,Viktor |
title |
Karyotype and COI gene sequence of Chironomus heteropilicornis Wülker, 1996 (Diptera, Chironomidae) from the Gydan Peninsula, Russia |
title_short |
Karyotype and COI gene sequence of Chironomus heteropilicornis Wülker, 1996 (Diptera, Chironomidae) from the Gydan Peninsula, Russia |
title_full |
Karyotype and COI gene sequence of Chironomus heteropilicornis Wülker, 1996 (Diptera, Chironomidae) from the Gydan Peninsula, Russia |
title_fullStr |
Karyotype and COI gene sequence of Chironomus heteropilicornis Wülker, 1996 (Diptera, Chironomidae) from the Gydan Peninsula, Russia |
title_full_unstemmed |
Karyotype and COI gene sequence of Chironomus heteropilicornis Wülker, 1996 (Diptera, Chironomidae) from the Gydan Peninsula, Russia |
title_sort |
karyotype and coi gene sequence of chironomus heteropilicornis wülker, 1996 (diptera, chironomidae) from the gydan peninsula, russia |
publisher |
Pensoft Publishers |
publishDate |
2021 |
url |
https://doi.org/10.3897/CompCytogen.v15i4.73135 https://compcytogen.pensoft.net/article/73135/ |
geographic |
Canada Greenland Norway |
geographic_facet |
Canada Greenland Norway |
genre |
Greenland |
genre_facet |
Greenland |
op_source |
Comparative Cytogenetics 15(4): 447-458 |
op_relation |
info:eu-repo/semantics/altIdentifier/eissn/1993-078X info:eu-repo/semantics/altIdentifier/pissn/1993-0771 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.3897/CompCytogen.v15i4.73135 |
container_title |
Comparative Cytogenetics |
container_volume |
15 |
container_issue |
4 |
container_start_page |
447 |
op_container_end_page |
458 |
_version_ |
1766019373159415808 |