Triacylglycerol biosynthesis in developing Ribes nigrum and Ribes rubrum seeds from gene expression to oil composition

• Published in: Food Chemistry
• Main Authors: Vuorinen, Anssi L., Kalpio, Marika, Linderborg, Kaisa M., Hoppula, Kati B., Karhu, Saila T., Yang, Baoru, Kallio, Heikki P.
• Other Authors: Vuorinen, AL (reprint author), Univ Turku, Dept Biochem, Food Chem & Food Dev, FI-20014 Turku, Finland., Univ Turku, Dept Biochem, Food Chem & Food Dev, FI-20014 Turku, Finland., Nat Resources Inst Finland, FI-88600 Sotkamo, Finland., Nat Resources Inst Finland, FI-21500 Piikkio, Finland., Peking Univ, Fac Publ Hlth, Dept Nutr & Food Hyg, Beijing 100191, Peoples R China., Univ Turku, Kevo Subarct Res Inst, FI-20014 Turku, Finland.
• Format: Journal/Newspaper
• Language: English
• Published: FOOD CHEMISTRY 2016
• Subjects: Ribes nigrum; Ribes rubrum; Gene expression; Fatty acid analysis; alpha-Linolenic acid; gamma-Linolenic acid; Stearidonic acid; FATTY-ACID; WEATHER CONDITIONS; PHENOLIC-COMPOUNDS; MASS-SPECTROMETRY; L. CULTIVARS; IDENTIFICATION; LATITUDE; LINOLENOYLDILINOLEOYLGLYCEROL; DELTA(6)-DESATURASE; Ribes; Northern Finland
• Online Access: https://hdl.handle.net/20.500.11897/416600; https://doi.org/10.1016/j.foodchem.2015.10.010

Oils with sufficient contents of fatty acids, which can be metabolized into precursors of anti-inflammatory eicosanoids, have potential health effects. Ribes sp. seed oil is rich in alpha-linolenic, gamma-linolenic and stearidonic acids belonging to this fatty acid group. Only a few previous studies exist on Ribes sp. gene expression. We followed the seed oil biosynthesis of four Ribes nigrum and two Ribes rubrum cultivars at different developmental stages over 2 years in Southern and Northern Finland with a 686 km latitudinal difference. The species and the developmental stage were the most important factors causing differences in gene expression levels and oil composition. Differences between cultivars were detected in some cases, but year and location had only small effects. However, expression of the gene encoding Delta(9)-desaturase in R. nigrum was affected by location. Triacylglycerol biosynthesis in Ribes sp. was distinctly buffered and typically followed a certain path, regardless of growth environment. (C) 2015 Elsevier Ltd. All rights reserved. Academy of Finland [251623] SCI(E) EI PubMed ARTICLE [e-mail] 976-987 196