Purification, characterisation and expression in Saccharomyces cerevisiae of LipG7 an enantioselective, cold-adapted lipase from the Antarctic filamentous fungus Geomyces sp P7 with unusual thermostability characteristics
A lipase, LipG7, has been purified from the Antarctic filamentous fungus Geomyces sp. P7 which was found to be cold-adapted and able to retain/regain its activity after heat denaturation. The LipG7 exhibits 100% residual activity following 1 h incubation at 100 C whilst simultaneously showing kineti...
Published in: | Enzyme and Microbial Technology |
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enzyme and microbial technology
2013
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Online Access: | https://hdl.handle.net/20.500.11897/222633 https://doi.org/10.1016/j.enzmictec.2013.03.021 |
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ftpekinguniv:oai:localhost:20.500.11897/222633 2023-05-15T13:30:32+02:00 Purification, characterisation and expression in Saccharomyces cerevisiae of LipG7 an enantioselective, cold-adapted lipase from the Antarctic filamentous fungus Geomyces sp P7 with unusual thermostability characteristics Florczak, Tomasz Daroch, Maurycy Wilkinson, Mark Charles Bialkowska, Aneta Bates, Andrew Derek Turkiewicz, Marianna Iwanejko, Lesley Ann Iwanejko, LA (reprint author), Univ Liverpool, Inst Integrat Biol, Biosci Bldg,Crown St, Liverpool L69 7ZB, Merseyside, England. Tech Univ Lodz, Inst Tech Biochem, Fac Biotechnol & Food Sci, PL-90924 Lodz, Poland. Univ Liverpool, Inst Integrat Biol, Liverpool L69 7ZB, Merseyside, England. Univ Liverpool, Dept Musculoskeletal Biol, Fac Hlth & Life Sci, Inst Ageing & Chron Dis, Liverpool L69 3GA, Merseyside, England. Peking Univ, Shenzhen Grad Sch, Sch Environm & Energy, Shenzhen 518055, Peoples R China. Univ Liverpool, Inst Integrat Biol, Biosci Bldg,Crown St, Liverpool L69 7ZB, Merseyside, England. 2013 https://hdl.handle.net/20.500.11897/222633 https://doi.org/10.1016/j.enzmictec.2013.03.021 en eng enzyme and microbial technology ENZYME AND MICROBIAL TECHNOLOGY.2013,53,(1),18-24. 821056 0141-0229 http://hdl.handle.net/20.500.11897/222633 doi:10.1016/j.enzmictec.2013.03.021 WOS:000320352100004 EI SCI Cold adaptation Thermostability Lipase Psychrophillic enzyme Inverse-PCR YARROWIA-LIPOLYTICA ACTIVE LIPASE EXTRACELLULAR LIPASE MICROBIAL LIPASES CLONING STRAIN GENE CLASSIFICATION BIOCATALYSIS EFFICIENCY Journal 2013 ftpekinguniv https://doi.org/20.500.11897/222633 https://doi.org/10.1016/j.enzmictec.2013.03.021 2021-08-01T08:39:23Z A lipase, LipG7, has been purified from the Antarctic filamentous fungus Geomyces sp. P7 which was found to be cold-adapted and able to retain/regain its activity after heat denaturation. The LipG7 exhibits 100% residual activity following 1 h incubation at 100 C whilst simultaneously showing kinetic adaptations to cold temperatures. LipG7 was also found to have industrial potential as an enantioselective biocatalyst as it is able to effectively catalyse the enantioselective transesterification of a secondary alcohol. The LipG7 coding sequence has been identified and cloned using 454 pyrosequencing of the transcriptome and inverse PCR. The LipG7 protein has been heterologously expressed in Saccharomyces cerevisiae BJ5465 and shown to exhibit the same characteristics as the native protein. (C) 2013 Elsevier Inc. All rights reserved. Biotechnology & Applied Microbiology SCI(E) EI 8 ARTICLE 1 18-24 53 Journal/Newspaper Antarc* Antarctic Peking University Institutional Repository (PKU IR) Antarctic The Antarctic Enzyme and Microbial Technology 53 1 18 24 |
institution |
Open Polar |
collection |
Peking University Institutional Repository (PKU IR) |
op_collection_id |
ftpekinguniv |
language |
English |
topic |
Cold adaptation Thermostability Lipase Psychrophillic enzyme Inverse-PCR YARROWIA-LIPOLYTICA ACTIVE LIPASE EXTRACELLULAR LIPASE MICROBIAL LIPASES CLONING STRAIN GENE CLASSIFICATION BIOCATALYSIS EFFICIENCY |
spellingShingle |
Cold adaptation Thermostability Lipase Psychrophillic enzyme Inverse-PCR YARROWIA-LIPOLYTICA ACTIVE LIPASE EXTRACELLULAR LIPASE MICROBIAL LIPASES CLONING STRAIN GENE CLASSIFICATION BIOCATALYSIS EFFICIENCY Florczak, Tomasz Daroch, Maurycy Wilkinson, Mark Charles Bialkowska, Aneta Bates, Andrew Derek Turkiewicz, Marianna Iwanejko, Lesley Ann Purification, characterisation and expression in Saccharomyces cerevisiae of LipG7 an enantioselective, cold-adapted lipase from the Antarctic filamentous fungus Geomyces sp P7 with unusual thermostability characteristics |
topic_facet |
Cold adaptation Thermostability Lipase Psychrophillic enzyme Inverse-PCR YARROWIA-LIPOLYTICA ACTIVE LIPASE EXTRACELLULAR LIPASE MICROBIAL LIPASES CLONING STRAIN GENE CLASSIFICATION BIOCATALYSIS EFFICIENCY |
description |
A lipase, LipG7, has been purified from the Antarctic filamentous fungus Geomyces sp. P7 which was found to be cold-adapted and able to retain/regain its activity after heat denaturation. The LipG7 exhibits 100% residual activity following 1 h incubation at 100 C whilst simultaneously showing kinetic adaptations to cold temperatures. LipG7 was also found to have industrial potential as an enantioselective biocatalyst as it is able to effectively catalyse the enantioselective transesterification of a secondary alcohol. The LipG7 coding sequence has been identified and cloned using 454 pyrosequencing of the transcriptome and inverse PCR. The LipG7 protein has been heterologously expressed in Saccharomyces cerevisiae BJ5465 and shown to exhibit the same characteristics as the native protein. (C) 2013 Elsevier Inc. All rights reserved. Biotechnology & Applied Microbiology SCI(E) EI 8 ARTICLE 1 18-24 53 |
author2 |
Iwanejko, LA (reprint author), Univ Liverpool, Inst Integrat Biol, Biosci Bldg,Crown St, Liverpool L69 7ZB, Merseyside, England. Tech Univ Lodz, Inst Tech Biochem, Fac Biotechnol & Food Sci, PL-90924 Lodz, Poland. Univ Liverpool, Inst Integrat Biol, Liverpool L69 7ZB, Merseyside, England. Univ Liverpool, Dept Musculoskeletal Biol, Fac Hlth & Life Sci, Inst Ageing & Chron Dis, Liverpool L69 3GA, Merseyside, England. Peking Univ, Shenzhen Grad Sch, Sch Environm & Energy, Shenzhen 518055, Peoples R China. Univ Liverpool, Inst Integrat Biol, Biosci Bldg,Crown St, Liverpool L69 7ZB, Merseyside, England. |
format |
Journal/Newspaper |
author |
Florczak, Tomasz Daroch, Maurycy Wilkinson, Mark Charles Bialkowska, Aneta Bates, Andrew Derek Turkiewicz, Marianna Iwanejko, Lesley Ann |
author_facet |
Florczak, Tomasz Daroch, Maurycy Wilkinson, Mark Charles Bialkowska, Aneta Bates, Andrew Derek Turkiewicz, Marianna Iwanejko, Lesley Ann |
author_sort |
Florczak, Tomasz |
title |
Purification, characterisation and expression in Saccharomyces cerevisiae of LipG7 an enantioselective, cold-adapted lipase from the Antarctic filamentous fungus Geomyces sp P7 with unusual thermostability characteristics |
title_short |
Purification, characterisation and expression in Saccharomyces cerevisiae of LipG7 an enantioselective, cold-adapted lipase from the Antarctic filamentous fungus Geomyces sp P7 with unusual thermostability characteristics |
title_full |
Purification, characterisation and expression in Saccharomyces cerevisiae of LipG7 an enantioselective, cold-adapted lipase from the Antarctic filamentous fungus Geomyces sp P7 with unusual thermostability characteristics |
title_fullStr |
Purification, characterisation and expression in Saccharomyces cerevisiae of LipG7 an enantioselective, cold-adapted lipase from the Antarctic filamentous fungus Geomyces sp P7 with unusual thermostability characteristics |
title_full_unstemmed |
Purification, characterisation and expression in Saccharomyces cerevisiae of LipG7 an enantioselective, cold-adapted lipase from the Antarctic filamentous fungus Geomyces sp P7 with unusual thermostability characteristics |
title_sort |
purification, characterisation and expression in saccharomyces cerevisiae of lipg7 an enantioselective, cold-adapted lipase from the antarctic filamentous fungus geomyces sp p7 with unusual thermostability characteristics |
publisher |
enzyme and microbial technology |
publishDate |
2013 |
url |
https://hdl.handle.net/20.500.11897/222633 https://doi.org/10.1016/j.enzmictec.2013.03.021 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
EI SCI |
op_relation |
ENZYME AND MICROBIAL TECHNOLOGY.2013,53,(1),18-24. 821056 0141-0229 http://hdl.handle.net/20.500.11897/222633 doi:10.1016/j.enzmictec.2013.03.021 WOS:000320352100004 |
op_doi |
https://doi.org/20.500.11897/222633 https://doi.org/10.1016/j.enzmictec.2013.03.021 |
container_title |
Enzyme and Microbial Technology |
container_volume |
53 |
container_issue |
1 |
container_start_page |
18 |
op_container_end_page |
24 |
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1766009716871266304 |