Purification, characterisation and expression in Saccharomyces cerevisiae of LipG7 an enantioselective, cold-adapted lipase from the Antarctic filamentous fungus Geomyces sp P7 with unusual thermostability characteristics

• Published in: Enzyme and Microbial Technology
• Main Authors: Florczak, Tomasz, Daroch, Maurycy, Wilkinson, Mark Charles, Bialkowska, Aneta, Bates, Andrew Derek, Turkiewicz, Marianna, Iwanejko, Lesley Ann
• Other Authors: Iwanejko, LA (reprint author), Univ Liverpool, Inst Integrat Biol, Biosci Bldg,Crown St, Liverpool L69 7ZB, Merseyside, England., Tech Univ Lodz, Inst Tech Biochem, Fac Biotechnol & Food Sci, PL-90924 Lodz, Poland., Univ Liverpool, Inst Integrat Biol, Liverpool L69 7ZB, Merseyside, England., Univ Liverpool, Dept Musculoskeletal Biol, Fac Hlth & Life Sci, Inst Ageing & Chron Dis, Liverpool L69 3GA, Merseyside, England., Peking Univ, Shenzhen Grad Sch, Sch Environm & Energy, Shenzhen 518055, Peoples R China., Univ Liverpool, Inst Integrat Biol, Biosci Bldg,Crown St, Liverpool L69 7ZB, Merseyside, England.
• Format: Journal/Newspaper
• Language: English
• Published: enzyme and microbial technology 2013
• Subjects: Cold adaptation; Thermostability; Lipase; Psychrophillic enzyme; Inverse-PCR; YARROWIA-LIPOLYTICA; ACTIVE LIPASE; EXTRACELLULAR LIPASE; MICROBIAL LIPASES; CLONING; STRAIN; GENE; CLASSIFICATION; BIOCATALYSIS; EFFICIENCY; Antarctic; The Antarctic; Antarc*
• Online Access: https://hdl.handle.net/20.500.11897/222633; https://doi.org/10.1016/j.enzmictec.2013.03.021

A lipase, LipG7, has been purified from the Antarctic filamentous fungus Geomyces sp. P7 which was found to be cold-adapted and able to retain/regain its activity after heat denaturation. The LipG7 exhibits 100% residual activity following 1 h incubation at 100 C whilst simultaneously showing kinetic adaptations to cold temperatures. LipG7 was also found to have industrial potential as an enantioselective biocatalyst as it is able to effectively catalyse the enantioselective transesterification of a secondary alcohol. The LipG7 coding sequence has been identified and cloned using 454 pyrosequencing of the transcriptome and inverse PCR. The LipG7 protein has been heterologously expressed in Saccharomyces cerevisiae BJ5465 and shown to exhibit the same characteristics as the native protein. (C) 2013 Elsevier Inc. All rights reserved. Biotechnology & Applied Microbiology SCI(E) EI 8 ARTICLE 1 18-24 53