Lipid-lowering effects of farnesylquinone and related analogues from the marine-derived Streptomyces nitrosporeus
Bioassay-guided fractionation of the fermentation broth of Arctic Streptomyces nitrosporeus YBH10-5 resulted in the isolation of seven new compounds named nitrosporeunols A-G (1-7), together with seven known analogues (8-14). Their structures were determined based on extensive spectroscopic analysis...
Published in: | Bioorganic & Medicinal Chemistry Letters |
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生物有机化学与医药化学通讯
2014
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Online Access: | https://hdl.handle.net/20.500.11897/156305 https://doi.org/10.1016/j.bmcl.2014.09.049 |
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ftpekinguniv:oai:localhost:20.500.11897/156305 2023-05-15T15:10:46+02:00 Lipid-lowering effects of farnesylquinone and related analogues from the marine-derived Streptomyces nitrosporeus Liu, Dong Yang, Aigang Wu, Chongming Guo, Peng Proksch, Peter Lin, Wenhan Guo, P (reprint author), Chinese Acad Med Sci, Peking Union Med Coll, Inst Med Plant Dev, Pharmacol & Toxicol Res Ctr, Beijing 100193, Peoples R China. Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China. Chinese Acad Med Sci, Peking Union Med Coll, Inst Med Plant Dev, Pharmacol & Toxicol Res Ctr, Beijing 100193, Peoples R China. Univ Dusseldorf, Inst Pharmazeut Biol & Biotechnol, D-40225 Dusseldorf, Germany. 2014 https://hdl.handle.net/20.500.11897/156305 https://doi.org/10.1016/j.bmcl.2014.09.049 en eng 生物有机化学与医药化学通讯 BIOORGANIC & MEDICINAL CHEMISTRY LETTERS.2014,24,(22),5288-5293. 652154 0960-894X http://hdl.handle.net/20.500.11897/156305 1464-3405 doi:10.1016/j.bmcl.2014.09.049 25304895 WOS:000343901400032 PubMed SCI Streptomyces nitrosporeus Nitrosporeunols A-G Farnesylquinone Lowering lipid effect PPAR alpha pathway CHOLESTEROL-METABOLISM DERIVATIVES ANTAGONISTS DISCOVERY LIPOLYSIS RECEPTOR PATHWAY CELLS Journal 2014 ftpekinguniv https://doi.org/20.500.11897/156305 https://doi.org/10.1016/j.bmcl.2014.09.049 2021-08-01T08:03:13Z Bioassay-guided fractionation of the fermentation broth of Arctic Streptomyces nitrosporeus YBH10-5 resulted in the isolation of seven new compounds named nitrosporeunols A-G (1-7), together with seven known analogues (8-14). Their structures were determined based on extensive spectroscopic analysis. Compounds 1-14 were evaluated for the lowering lipid effects, while two compounds (10 and 12) remarkably decreased lipid levels including total cholesterol (TC) and triglycerides (TG) in HepG2 cells. Quantitative realtime PCR and Western blot indicated that farnesylquinone (12) increased the expression of the key proteins including peroxisome proliferator-activated receptor-alpha (PPAR alpha), peroxisome proliferator-activated receptor-gamma, and coactivator 1 alpha (PGC-1 alpha), as well as their downstream genes carnitine palmitoyltransterase-1 (CPT-1), acyl-coenzyme A oxidase 1 (ACOX), malonyl CoA decarboxylase 1 (MCD1), pyruvate dehydrogenase kinase 4 (PDK4), and cholesterol 7 alpha-hydroxylase (CYP7A1). Luciferase assay showed that 12 increased the transcriptional activity of PPAR alpha, while its lipid-lowering effect was abolished by PPAR alpha inhibitor, MK886, in HepG2 cells. These findings suggested that 12 is a potent lipid-lowering agent which may decrease lipid levels through upregulation of PPAR alpha pathway. (C) 2014 Elsevier Ltd. All rights reserved. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000343901400032&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 Chemistry, Medicinal Chemistry, Organic SCI(E) PubMed 2 ARTICLE pguo@implad.ac.cn; whlin@bjmu.edu.cn 22 5288-5293 24 Journal/Newspaper Arctic Peking University Institutional Repository (PKU IR) Arctic Bioorganic & Medicinal Chemistry Letters 24 22 5288 5293 |
institution |
Open Polar |
collection |
Peking University Institutional Repository (PKU IR) |
op_collection_id |
ftpekinguniv |
language |
English |
topic |
Streptomyces nitrosporeus Nitrosporeunols A-G Farnesylquinone Lowering lipid effect PPAR alpha pathway CHOLESTEROL-METABOLISM DERIVATIVES ANTAGONISTS DISCOVERY LIPOLYSIS RECEPTOR PATHWAY CELLS |
spellingShingle |
Streptomyces nitrosporeus Nitrosporeunols A-G Farnesylquinone Lowering lipid effect PPAR alpha pathway CHOLESTEROL-METABOLISM DERIVATIVES ANTAGONISTS DISCOVERY LIPOLYSIS RECEPTOR PATHWAY CELLS Liu, Dong Yang, Aigang Wu, Chongming Guo, Peng Proksch, Peter Lin, Wenhan Lipid-lowering effects of farnesylquinone and related analogues from the marine-derived Streptomyces nitrosporeus |
topic_facet |
Streptomyces nitrosporeus Nitrosporeunols A-G Farnesylquinone Lowering lipid effect PPAR alpha pathway CHOLESTEROL-METABOLISM DERIVATIVES ANTAGONISTS DISCOVERY LIPOLYSIS RECEPTOR PATHWAY CELLS |
description |
Bioassay-guided fractionation of the fermentation broth of Arctic Streptomyces nitrosporeus YBH10-5 resulted in the isolation of seven new compounds named nitrosporeunols A-G (1-7), together with seven known analogues (8-14). Their structures were determined based on extensive spectroscopic analysis. Compounds 1-14 were evaluated for the lowering lipid effects, while two compounds (10 and 12) remarkably decreased lipid levels including total cholesterol (TC) and triglycerides (TG) in HepG2 cells. Quantitative realtime PCR and Western blot indicated that farnesylquinone (12) increased the expression of the key proteins including peroxisome proliferator-activated receptor-alpha (PPAR alpha), peroxisome proliferator-activated receptor-gamma, and coactivator 1 alpha (PGC-1 alpha), as well as their downstream genes carnitine palmitoyltransterase-1 (CPT-1), acyl-coenzyme A oxidase 1 (ACOX), malonyl CoA decarboxylase 1 (MCD1), pyruvate dehydrogenase kinase 4 (PDK4), and cholesterol 7 alpha-hydroxylase (CYP7A1). Luciferase assay showed that 12 increased the transcriptional activity of PPAR alpha, while its lipid-lowering effect was abolished by PPAR alpha inhibitor, MK886, in HepG2 cells. These findings suggested that 12 is a potent lipid-lowering agent which may decrease lipid levels through upregulation of PPAR alpha pathway. (C) 2014 Elsevier Ltd. All rights reserved. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000343901400032&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 Chemistry, Medicinal Chemistry, Organic SCI(E) PubMed 2 ARTICLE pguo@implad.ac.cn; whlin@bjmu.edu.cn 22 5288-5293 24 |
author2 |
Guo, P (reprint author), Chinese Acad Med Sci, Peking Union Med Coll, Inst Med Plant Dev, Pharmacol & Toxicol Res Ctr, Beijing 100193, Peoples R China. Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China. Chinese Acad Med Sci, Peking Union Med Coll, Inst Med Plant Dev, Pharmacol & Toxicol Res Ctr, Beijing 100193, Peoples R China. Univ Dusseldorf, Inst Pharmazeut Biol & Biotechnol, D-40225 Dusseldorf, Germany. |
format |
Journal/Newspaper |
author |
Liu, Dong Yang, Aigang Wu, Chongming Guo, Peng Proksch, Peter Lin, Wenhan |
author_facet |
Liu, Dong Yang, Aigang Wu, Chongming Guo, Peng Proksch, Peter Lin, Wenhan |
author_sort |
Liu, Dong |
title |
Lipid-lowering effects of farnesylquinone and related analogues from the marine-derived Streptomyces nitrosporeus |
title_short |
Lipid-lowering effects of farnesylquinone and related analogues from the marine-derived Streptomyces nitrosporeus |
title_full |
Lipid-lowering effects of farnesylquinone and related analogues from the marine-derived Streptomyces nitrosporeus |
title_fullStr |
Lipid-lowering effects of farnesylquinone and related analogues from the marine-derived Streptomyces nitrosporeus |
title_full_unstemmed |
Lipid-lowering effects of farnesylquinone and related analogues from the marine-derived Streptomyces nitrosporeus |
title_sort |
lipid-lowering effects of farnesylquinone and related analogues from the marine-derived streptomyces nitrosporeus |
publisher |
生物有机化学与医药化学通讯 |
publishDate |
2014 |
url |
https://hdl.handle.net/20.500.11897/156305 https://doi.org/10.1016/j.bmcl.2014.09.049 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PubMed SCI |
op_relation |
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS.2014,24,(22),5288-5293. 652154 0960-894X http://hdl.handle.net/20.500.11897/156305 1464-3405 doi:10.1016/j.bmcl.2014.09.049 25304895 WOS:000343901400032 |
op_doi |
https://doi.org/20.500.11897/156305 https://doi.org/10.1016/j.bmcl.2014.09.049 |
container_title |
Bioorganic & Medicinal Chemistry Letters |
container_volume |
24 |
container_issue |
22 |
container_start_page |
5288 |
op_container_end_page |
5293 |
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1766341726090297344 |