Aquarium monitoring during a land-based experiment testing the cumulative effect of Cu exposure and ocean acidification on cold-water coral Viminella flagellum

• Main Authors: Martins, Ines, Mano, Beatriz, Godinho, Antonio, Goulart, Joana, Sire de Vilar, Anaïs, Carreiro-Silva, Marina
• Format: Dataset
• Language: English
• Published: PANGAEA 2024
• Subjects: Azores; Azores_Deep_Octocoral_Sampling_Viminella_flagellum; Chamber number; Condor Seamounts; Experiment; Glass electrode (Crison pH 25+); iAtlantic; Integrated Assessment of Atlantic Marine Ecosystems in Space and Time; Laboratory experiment; Oxygen; Oxygen Dipping Probe; DP-PSt3; Fibox 4; PreSens; pH; Sample ID; Species; unique identification; unique identification (Semantic URI); unique identification (URI); Temperature; water; Time in days; Treatment; Viminella_flagellum_Aquaria_Experiment; Ocean acidification
• Online Access: https://doi.pangaea.de/10.1594/PANGAEA.966750; https://doi.org/10.1594/PANGAEA.966750

We report the results of a land-based experiment testing the cumulative effects of copper (Cu) exposure and ocean acidification (OA). Corals were obtained as by-catch during an experimental long-line fisheries campaign (CONDOR monitoring program, Okeanos-University of the Azores) on the Condor Seamount (38°54′N, 29°05′W), Azores Archipelago, at 200 m depth in October 2019. The experiment was undertaken at the DeepSeaLab aquaria facilities (Okeanos-University of the Azores) where corals were exposed to four OA/Cu-contamination scenarios: (1) ambient pCO2/pH level as measured in situ conditions (385 μatm/ pH 8.09); (2) high pCO2/reduced pH (IPCC RCP8.5 scenario, 1000 μatm/ pH 7.73); (3) ambient pCO2/pH level and additional Cu concentration (60 µg/l); (4) high pCO2/reduced pH and additional Cu concentration (60 µg/l). The pH/pCO2 modification was achieved by bubbling seawater with either pure CO2 (to increase pCO2) or CO2 low air (to decrease pCO2). The copper concentration used in this experiment followed a trial simulating a polymetallic particles plume release during a potential deep-sea mining event. This sublethal concentration was found to be the highest copper concentration dissolved in seawater. Seawater physical-chemical parameters were measured daily in each chamber. Temperature, pH and oxygen saturation were measured manually in each aquaria every day using a Mettler-Toledo S8 glass and a Fibox4 (PreSens) with an Oxygen Probe PSt3.