Flow cytometry dataset from first year sea ice (FYI) core bottom 5 cm sections showing the abundance of microorganisms (< 20 µm) during leg 2, 3 (February, March and April 2020) of the Arctic MOSAiC expedition

• Main Authors: Müller, Oliver, Fong, Allison A, Olsen, Lasse Mørk, Hoppe, Clara Jule Marie, Torstensson, Anders, Heitman, Laura, Petelenz, Elzbieta, Larsen, Aud, Tsagaraki, Tatiana M, Mayers, Kyle, Langvad, Maria, Granskog, Mats A, Bratbak, Gunnar
• Format: Dataset
• Language: English
• Published: PANGAEA 2023
• Subjects: Acoustic focusing cytometer; Thermo Fisher; Attune NxT [20 mW 488 nm (blue) laser]; Activity description; Arctic Ocean; Core length; DATE/TIME; Depth; bathymetric; ice/snow; Device type; Event label; Factor; Feature; flow cytometry; Flow cytometry system; Becton Dickinson; FACSCalibur; HAVOC; Heterotrophic nanophytoplankton; Heterotrophic prokaryotes; large; medium; very large; Heterotrophic prokaryotes with relatively high nucleic acid; Heterotrophic prokaryotes with relatively low nucleic acid; IC; Ice core; Ice corer; Identification; LATITUDE; Leg Number; LONGITUDE; microbial abundance; MOSAiC; MOSAiC_ECO; MOSAiC20192020; MOSAiC expedition; Multidisciplinary drifting Observatory for the Study of Arctic Climate; Orange and red fluorescing nanophytoplankton; Orange and red fluorescing picophytoplankton; Orange fluorescing prokaryote picophytoplankton; Polarstern; PS122/2; PS122/2_23-3; PS122/2_24-8; Arctic; ice core; Sea ice
• Online Access: https://doi.pangaea.de/10.1594/PANGAEA.963560; https://doi.org/10.1594/PANGAEA.963560

This dataset is a subset of the abundance of microorganisms (smaller than 20 µm) enumerated using flow cytometry (FCM) during the Multidisciplinary drifting observatory for the study of Arctic Climate (MOSAiC) sampled from first year sea ice (FYI) core bottom 5 cm sections from leg 2 and 3 (February, March, April 2020). For sea ice derived FCM abundance data, subsamples of 15 mL were taken from pooled ice core sections that were melted in filtered sea water and correspondingly a correction factor applied (details provided in the data-file), to enumerate the abundance of microorganisms per mL of melted sea ice. Additional expedition and sampling details can be found in the ECO-overview paper (Fong et al., to be submitted to Elementa). We thank all persons involved in the expedition of the Research Vessel Polarstern during MOSAiC in 2019-2020 (AWI_PS122_00) as listed in Nixdorf et al. (2021). Flow cytometry (FCM) is a fast, high-throughput method to enumerate the abundance of microorganism (smaller than 20 µm). FCM uses the hydrodynamic focusing of a laminar flow to separate and line up microscopic particles. When particles pass a laser beam, the generated light scattering can be used to estimate their cell size, obtain information about cell granularity and surface characteristics and determine fluorescence from inherent pigments or applied stains, such as DNA binding ones. Photosynthetic microorganisms have auto-fluorescent pigments, such as chlorophylls which in combination with the light scattering properties (cell size) or surface properties, can be used to group them into clusters of similar or identical organism types. Heterotrophic microorganisms, including archaea, bacteria and heterotrophic nanoflagellates, and virus do not have fluorescent pigments and require staining, for example using SYBR Green to stain Nucleic Acids (DNA) in order to distinguish these cells from other organic and inorganic particles in the sample. Samples for flow cytometric analysis were taken in triplicates or quadruplicates of ...