Temperature response curves of Emiliania huxleyi during laboratory experiments
At the end of the two year incubation of a subantarctic E. huxleyi culture (2015-11-20 to 2017-12-01), a temperature response experiment was performed using the Now (Day 747) and Future (Day 725) populations. Now and Future populations were grown in triplicate 28 ml Nalgene Oak Ridge-style centrifug...
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.959835 2024-09-09T20:01:40+00:00 Temperature response curves of Emiliania huxleyi during laboratory experiments Armstrong, Evelyn Law, Cliff S LATITUDE: -45.829000 * LONGITUDE: 171.532000 * DATE/TIME START: 2014-06-03T00:00:00 * DATE/TIME END: 2014-06-03T00:00:00 2023 text/tab-separated-values, 297 data points https://doi.pangaea.de/10.1594/PANGAEA.959835 https://doi.org/10.1594/PANGAEA.959835 en eng PANGAEA https://doi.org/10.1594/PANGAEA.959857 Armstrong, Evelyn; Law, Cliff S (2023): Resilience of Emiliania huxleyi to future changes in subantarctic waters. PLoS ONE, 18(11), e0284415, https://doi.org/10.1371/journal.pone.0284415 Thomas, William H; Scotten, Harold L; Bradshaw, John S (1963): Thermal gradient incubators for small aquatic organisms. Limnology and Oceanography, 8(3), 357-360, https://doi.org/10.4319/lo.1963.8.3.0357 https://doi.pangaea.de/10.1594/PANGAEA.959835 https://doi.org/10.1594/PANGAEA.959835 CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess adaptation Bottle Niskin Emiliania_huxleyi_subantarctic Emiliania huxleyi growth rate Fluorometer Turner Designs 10-AU Laboratory experiment NIS Ocean acidification ocean warming P140603 Polaris II Species unique identification unique identification (Semantic URI) unique identification (URI) subantarctic Temperature water Treatment Type of study dataset 2023 ftpangaea https://doi.org/10.1594/PANGAEA.95983510.1594/PANGAEA.95985710.1371/journal.pone.028441510.4319/lo.1963.8.3.0357 2024-07-24T02:31:43Z At the end of the two year incubation of a subantarctic E. huxleyi culture (2015-11-20 to 2017-12-01), a temperature response experiment was performed using the Now (Day 747) and Future (Day 725) populations. Now and Future populations were grown in triplicate 28 ml Nalgene Oak Ridge-style centrifuge tubes (Sigma) in Now or Future medium, respectively. The tubes were held in an aluminium temperature gradient block similar to Thomas et al. (1963), that was heated at one end and cooled at the other using water maintained at constant temperature by refrigerated circulators (Julabo GmbH). The temperature regime resulted in a range of 10 temperatures along the length of the block from 6.2°C at one end to 16.5°C at the other, with a range within replicates at each temperature of 0.1 – 0.2°C. The block was lit from below which achieved a light level of 62 +/- 7 μmol m⁻² s⁻¹ at the bottom of the tubes in a 12 hour: 12 hour light: dark cycle. To ensure uniform light each replicate was manually rotated through the row holes at the set temperature during the incubation period. Measurements of in vivo chl-a were made daily and growth rates calculated from the least squares regression of the natural logarithm of in vivo fluorescence, versus time. Dataset Ocean acidification PANGAEA - Data Publisher for Earth & Environmental Science ENVELOPE(171.532000,171.532000,-45.829000,-45.829000) |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
adaptation Bottle Niskin Emiliania_huxleyi_subantarctic Emiliania huxleyi growth rate Fluorometer Turner Designs 10-AU Laboratory experiment NIS Ocean acidification ocean warming P140603 Polaris II Species unique identification unique identification (Semantic URI) unique identification (URI) subantarctic Temperature water Treatment Type of study |
spellingShingle |
adaptation Bottle Niskin Emiliania_huxleyi_subantarctic Emiliania huxleyi growth rate Fluorometer Turner Designs 10-AU Laboratory experiment NIS Ocean acidification ocean warming P140603 Polaris II Species unique identification unique identification (Semantic URI) unique identification (URI) subantarctic Temperature water Treatment Type of study Armstrong, Evelyn Law, Cliff S Temperature response curves of Emiliania huxleyi during laboratory experiments |
topic_facet |
adaptation Bottle Niskin Emiliania_huxleyi_subantarctic Emiliania huxleyi growth rate Fluorometer Turner Designs 10-AU Laboratory experiment NIS Ocean acidification ocean warming P140603 Polaris II Species unique identification unique identification (Semantic URI) unique identification (URI) subantarctic Temperature water Treatment Type of study |
description |
At the end of the two year incubation of a subantarctic E. huxleyi culture (2015-11-20 to 2017-12-01), a temperature response experiment was performed using the Now (Day 747) and Future (Day 725) populations. Now and Future populations were grown in triplicate 28 ml Nalgene Oak Ridge-style centrifuge tubes (Sigma) in Now or Future medium, respectively. The tubes were held in an aluminium temperature gradient block similar to Thomas et al. (1963), that was heated at one end and cooled at the other using water maintained at constant temperature by refrigerated circulators (Julabo GmbH). The temperature regime resulted in a range of 10 temperatures along the length of the block from 6.2°C at one end to 16.5°C at the other, with a range within replicates at each temperature of 0.1 – 0.2°C. The block was lit from below which achieved a light level of 62 +/- 7 μmol m⁻² s⁻¹ at the bottom of the tubes in a 12 hour: 12 hour light: dark cycle. To ensure uniform light each replicate was manually rotated through the row holes at the set temperature during the incubation period. Measurements of in vivo chl-a were made daily and growth rates calculated from the least squares regression of the natural logarithm of in vivo fluorescence, versus time. |
format |
Dataset |
author |
Armstrong, Evelyn Law, Cliff S |
author_facet |
Armstrong, Evelyn Law, Cliff S |
author_sort |
Armstrong, Evelyn |
title |
Temperature response curves of Emiliania huxleyi during laboratory experiments |
title_short |
Temperature response curves of Emiliania huxleyi during laboratory experiments |
title_full |
Temperature response curves of Emiliania huxleyi during laboratory experiments |
title_fullStr |
Temperature response curves of Emiliania huxleyi during laboratory experiments |
title_full_unstemmed |
Temperature response curves of Emiliania huxleyi during laboratory experiments |
title_sort |
temperature response curves of emiliania huxleyi during laboratory experiments |
publisher |
PANGAEA |
publishDate |
2023 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.959835 https://doi.org/10.1594/PANGAEA.959835 |
op_coverage |
LATITUDE: -45.829000 * LONGITUDE: 171.532000 * DATE/TIME START: 2014-06-03T00:00:00 * DATE/TIME END: 2014-06-03T00:00:00 |
long_lat |
ENVELOPE(171.532000,171.532000,-45.829000,-45.829000) |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
https://doi.org/10.1594/PANGAEA.959857 Armstrong, Evelyn; Law, Cliff S (2023): Resilience of Emiliania huxleyi to future changes in subantarctic waters. PLoS ONE, 18(11), e0284415, https://doi.org/10.1371/journal.pone.0284415 Thomas, William H; Scotten, Harold L; Bradshaw, John S (1963): Thermal gradient incubators for small aquatic organisms. Limnology and Oceanography, 8(3), 357-360, https://doi.org/10.4319/lo.1963.8.3.0357 https://doi.pangaea.de/10.1594/PANGAEA.959835 https://doi.org/10.1594/PANGAEA.959835 |
op_rights |
CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1594/PANGAEA.95983510.1594/PANGAEA.95985710.1371/journal.pone.028441510.4319/lo.1963.8.3.0357 |
_version_ |
1809933548158713856 |