Cell volume of Emiliania huxleyi measured at cross-over points during laboratory experiments
On four occasions during the two year incubation of a subantarctic E. huxleyi culture (2015-11-20 to 2017-12-01), from Day 309 onwards, cell size of the cultures was measured at cross-over points, after 7 – 8 generations while still in exponential growth phase. Cells from Now (11°C and pH 8.1) and F...
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.959827 2024-09-09T20:01:35+00:00 Cell volume of Emiliania huxleyi measured at cross-over points during laboratory experiments Armstrong, Evelyn Law, Cliff S LATITUDE: -45.829000 * LONGITUDE: 171.532000 * DATE/TIME START: 2014-06-03T00:00:00 * DATE/TIME END: 2014-06-03T00:00:00 2023 text/tab-separated-values, 285 data points https://doi.pangaea.de/10.1594/PANGAEA.959827 https://doi.org/10.1594/PANGAEA.959827 en eng PANGAEA https://doi.org/10.1594/PANGAEA.959857 Armstrong, Evelyn; Law, Cliff S (2023): Resilience of Emiliania huxleyi to future changes in subantarctic waters. PLoS ONE, 18(11), e0284415, https://doi.org/10.1371/journal.pone.0284415 Buitenhuis, Erik Theodoor; Pangerc, Tanja; Franklin, Daniel J; Le Quéré, Corinne; Malin, Gill (2008): Growth rates of six coccolithophorid strains as a function of temperature. Limnology and Oceanography, 53(3), 1181-1185, https://doi.org/10.4319/lo.2008.53.3.1181 https://doi.pangaea.de/10.1594/PANGAEA.959827 https://doi.org/10.1594/PANGAEA.959827 CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess adaptation Bottle Niskin Comment Emiliania_huxleyi_subantarctic Emiliania huxleyi cell biovolume Inverted tissue culture microscope Olympus IX70 Laboratory experiment NIS Ocean acidification ocean warming P140603 Polaris II Species unique identification unique identification (Semantic URI) unique identification (URI) subantarctic Time in days Treatment Type of study dataset 2023 ftpangaea https://doi.org/10.1594/PANGAEA.95982710.1594/PANGAEA.95985710.1371/journal.pone.028441510.4319/lo.2008.53.3.1181 2024-07-24T02:31:43Z On four occasions during the two year incubation of a subantarctic E. huxleyi culture (2015-11-20 to 2017-12-01), from Day 309 onwards, cell size of the cultures was measured at cross-over points, after 7 – 8 generations while still in exponential growth phase. Cells from Now (11°C and pH 8.1) and Future (14°C and pH 7.8) cultures, and also the crossover experiments - Future cells inoculated into Now medium and incubated under Now conditions (Future in Now), and Now cells incubated in Future medium under Future conditions (Now in Future) were fixed in 1 % glutaraldehyde. Subsamples were mounted in a nanoplankton chamber (Phytotech) and measured with a calibrated eye-piece graticule under an Olympus IX70 inverted microscope. As the fixation process caused coccoliths to separate, the cell size measurements were for the cell body only, which allows more accurate determinations of cell volume (Buitenhuis et al., 2008). Cell volume was calculated assuming that the cells were spherical. Dataset Ocean acidification PANGAEA - Data Publisher for Earth & Environmental Science Olympus ENVELOPE(156.767,156.767,-80.217,-80.217) ENVELOPE(171.532000,171.532000,-45.829000,-45.829000) |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
adaptation Bottle Niskin Comment Emiliania_huxleyi_subantarctic Emiliania huxleyi cell biovolume Inverted tissue culture microscope Olympus IX70 Laboratory experiment NIS Ocean acidification ocean warming P140603 Polaris II Species unique identification unique identification (Semantic URI) unique identification (URI) subantarctic Time in days Treatment Type of study |
spellingShingle |
adaptation Bottle Niskin Comment Emiliania_huxleyi_subantarctic Emiliania huxleyi cell biovolume Inverted tissue culture microscope Olympus IX70 Laboratory experiment NIS Ocean acidification ocean warming P140603 Polaris II Species unique identification unique identification (Semantic URI) unique identification (URI) subantarctic Time in days Treatment Type of study Armstrong, Evelyn Law, Cliff S Cell volume of Emiliania huxleyi measured at cross-over points during laboratory experiments |
topic_facet |
adaptation Bottle Niskin Comment Emiliania_huxleyi_subantarctic Emiliania huxleyi cell biovolume Inverted tissue culture microscope Olympus IX70 Laboratory experiment NIS Ocean acidification ocean warming P140603 Polaris II Species unique identification unique identification (Semantic URI) unique identification (URI) subantarctic Time in days Treatment Type of study |
description |
On four occasions during the two year incubation of a subantarctic E. huxleyi culture (2015-11-20 to 2017-12-01), from Day 309 onwards, cell size of the cultures was measured at cross-over points, after 7 – 8 generations while still in exponential growth phase. Cells from Now (11°C and pH 8.1) and Future (14°C and pH 7.8) cultures, and also the crossover experiments - Future cells inoculated into Now medium and incubated under Now conditions (Future in Now), and Now cells incubated in Future medium under Future conditions (Now in Future) were fixed in 1 % glutaraldehyde. Subsamples were mounted in a nanoplankton chamber (Phytotech) and measured with a calibrated eye-piece graticule under an Olympus IX70 inverted microscope. As the fixation process caused coccoliths to separate, the cell size measurements were for the cell body only, which allows more accurate determinations of cell volume (Buitenhuis et al., 2008). Cell volume was calculated assuming that the cells were spherical. |
format |
Dataset |
author |
Armstrong, Evelyn Law, Cliff S |
author_facet |
Armstrong, Evelyn Law, Cliff S |
author_sort |
Armstrong, Evelyn |
title |
Cell volume of Emiliania huxleyi measured at cross-over points during laboratory experiments |
title_short |
Cell volume of Emiliania huxleyi measured at cross-over points during laboratory experiments |
title_full |
Cell volume of Emiliania huxleyi measured at cross-over points during laboratory experiments |
title_fullStr |
Cell volume of Emiliania huxleyi measured at cross-over points during laboratory experiments |
title_full_unstemmed |
Cell volume of Emiliania huxleyi measured at cross-over points during laboratory experiments |
title_sort |
cell volume of emiliania huxleyi measured at cross-over points during laboratory experiments |
publisher |
PANGAEA |
publishDate |
2023 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.959827 https://doi.org/10.1594/PANGAEA.959827 |
op_coverage |
LATITUDE: -45.829000 * LONGITUDE: 171.532000 * DATE/TIME START: 2014-06-03T00:00:00 * DATE/TIME END: 2014-06-03T00:00:00 |
long_lat |
ENVELOPE(156.767,156.767,-80.217,-80.217) ENVELOPE(171.532000,171.532000,-45.829000,-45.829000) |
geographic |
Olympus |
geographic_facet |
Olympus |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
https://doi.org/10.1594/PANGAEA.959857 Armstrong, Evelyn; Law, Cliff S (2023): Resilience of Emiliania huxleyi to future changes in subantarctic waters. PLoS ONE, 18(11), e0284415, https://doi.org/10.1371/journal.pone.0284415 Buitenhuis, Erik Theodoor; Pangerc, Tanja; Franklin, Daniel J; Le Quéré, Corinne; Malin, Gill (2008): Growth rates of six coccolithophorid strains as a function of temperature. Limnology and Oceanography, 53(3), 1181-1185, https://doi.org/10.4319/lo.2008.53.3.1181 https://doi.pangaea.de/10.1594/PANGAEA.959827 https://doi.org/10.1594/PANGAEA.959827 |
op_rights |
CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1594/PANGAEA.95982710.1594/PANGAEA.95985710.1371/journal.pone.028441510.4319/lo.2008.53.3.1181 |
_version_ |
1809933468845473792 |