Single prokaryotic cell abundances of the Northeast Greenland (NEG) shelf sediments from POLARSTERN cruise PS109

• Main Authors: Bodur, Yasemin V, Felden, Janine, Braeckman, Ulrike
• Format: Dataset
• Language: English
• Published: PANGAEA 2024
• Subjects: Acridine Orange Direct Counting (AODC); AODC; ARK-XXXI/4; B_LANDER; Bottom lander; Counts; Date/Time of event; Depth; bottom/max; sediment/rock; top/min; Elevation of event; Estimated from counts according to Hobbie et al. (1977); Event label; Filter; FRAM; FRontiers in Arctic marine Monitoring; Latitude of event; Longitude of event; Method/Device of event; Microbial abundance; cells; Multicorer with television; Number of cells; Polarstern; PS109; PS109_105-2; PS109_115-2; PS109_122-1; PS109_125-3; PS109_139-2; PS109_139-3; PS109_154-1; PS109_19-4; PS109_36-2; PS109_45-4; PS109_61-1; PS109_62-1; PS109_68-1; PS109_69-1; PS109_76-2; PS109_93-2; Sample number; Standard deviation; TVMUC; Arctic; Greenland
• Online Access: https://doi.pangaea.de/10.1594/PANGAEA.959552; https://doi.org/10.1594/PANGAEA.959552

Samples for single cell abundances as an estimate for the abundance of benthic bacteria on the Northeast Greenland shelf have been collected with R/V Polarstern during PS109 between September and October 2017 using a camera-equipped multiple corer (TV-MUC; core area 0.007 m²). Upon arrival on deck, the upper 5 cm of three MUC cores were sliced into 1 cm layers with a 10 ml cut-off syringe. 2 ml of each slice was transferred into a scintillation vial and fixed with 2 % filtered formaldehyde-seawater solution. In the laboratory, the samples were diluted, filtered through polycarbonate filters (0.2 µm, Whatman Nucleopore Track-Etch Membrane) and stained with a 0.001 % acridine orange solution after Hobbie et al., 1977 (doi:10.1128/aem.33.5.1225-1228.1977). Cells were counted on at least 30 grids (125 µm * 125 µm area) for 2 replicate filters per sample each with a Zeiss Axiophot microscope (Germany) and a 100x oil immersion objective lens (Zeiss Plan-Apochromat, Germany).