Seawater carbonate chemistry and phenotypic Plasticity in Crassostrea virginica Larvae
Ocean acidification (OA) is a major threat to marine calcifiers, and little is known regarding acclimation to OA in bivalves. This study combined physiological assays with next-generation sequencing to assess the potential for recovery from and acclimation to OA in the eastern oyster (Crassostrea vi...
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.957780 2023-06-11T04:15:05+02:00 Seawater carbonate chemistry and phenotypic Plasticity in Crassostrea virginica Larvae Barbosa, Michelle Schwaner, Caroline Espinosa, Emmanuelle Pales Allam, Bassem MEDIAN LATITUDE: 41.168275 * MEDIAN LONGITUDE: -72.212275 * SOUTH-BOUND LATITUDE: 40.884000 * WEST-BOUND LONGITUDE: -73.343200 * NORTH-BOUND LATITUDE: 41.930500 * EAST-BOUND LONGITUDE: -70.031000 2023-04-23 text/tab-separated-values, 1912 data points https://doi.pangaea.de/10.1594/PANGAEA.957780 en eng PANGAEA Barbosa, Michelle; Schwaner, Caroline; Espinosa, Emmanuelle Pales; Allam, Bassem (2022): A Transcriptomic Analysis of Phenotypic Plasticity in Crassostrea virginica Larvae under Experimental Acidification. Genes, 13(9), 1529, https://doi.org/10.3390/genes13091529 Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2022): seacarb: seawater carbonate chemistry with R. R package version 3.3.1. https://cran.r-project.org/web/packages/seacarb/index.html https://doi.pangaea.de/10.1594/PANGAEA.957780 CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess Alkalinity total standard deviation Animalia Aragonite saturation state Bicarbonate ion Calcite saturation state Calculated using seacarb Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Coast and continental shelf Containers and aquaria (20-1000 L or < 1 m**2) Coulometric titration Crassostrea virginica EXP Experiment Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Growth/Morphology Identification Laboratory experiment Length Mollusca Mortality Mortality/Survival Mount_Sinai_Harbor North Atlantic Northport_NY OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos Dataset 2023 ftpangaea https://doi.org/10.3390/genes13091529 2023-04-26T05:38:36Z Ocean acidification (OA) is a major threat to marine calcifiers, and little is known regarding acclimation to OA in bivalves. This study combined physiological assays with next-generation sequencing to assess the potential for recovery from and acclimation to OA in the eastern oyster (Crassostrea virginica) and identify molecular mechanisms associated with resilience. In a reciprocal transplant experiment, larvae transplanted from elevated pCO2 (~1400 ppm) to ambient pCO2 (~350 ppm) demonstrated significantly lower mortality and larger size post-transplant than oysters remaining under elevated pCO2 and had similar mortality compared to those remaining in ambient conditions. The recovery after transplantation to ambient conditions demonstrates the ability for larvae to rebound and suggests phenotypic plasticity and acclimation. Transcriptomic analysis supported this hypothesis as genes were differentially regulated under OA stress. Transcriptomic profiles of transplanted and non-transplanted larvae terminating in the same final pCO2 converged, further supporting the idea that acclimation underlies resilience. The functions of differentially expressed genes included cell differentiation, development, biomineralization, ion exchange, and immunity. Results suggest acclimation as a mode of resilience to OA. In addition, the identification of genes associated with resilience can serve as a valuable resource for the aquaculture industry, as these could enable marker-assisted selection of OA-resilient stocks. Dataset North Atlantic Ocean acidification PANGAEA - Data Publisher for Earth & Environmental Science ENVELOPE(-73.343200,-70.031000,41.930500,40.884000) |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
Alkalinity total standard deviation Animalia Aragonite saturation state Bicarbonate ion Calcite saturation state Calculated using seacarb Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Coast and continental shelf Containers and aquaria (20-1000 L or < 1 m**2) Coulometric titration Crassostrea virginica EXP Experiment Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Growth/Morphology Identification Laboratory experiment Length Mollusca Mortality Mortality/Survival Mount_Sinai_Harbor North Atlantic Northport_NY OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos |
spellingShingle |
Alkalinity total standard deviation Animalia Aragonite saturation state Bicarbonate ion Calcite saturation state Calculated using seacarb Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Coast and continental shelf Containers and aquaria (20-1000 L or < 1 m**2) Coulometric titration Crassostrea virginica EXP Experiment Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Growth/Morphology Identification Laboratory experiment Length Mollusca Mortality Mortality/Survival Mount_Sinai_Harbor North Atlantic Northport_NY OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos Barbosa, Michelle Schwaner, Caroline Espinosa, Emmanuelle Pales Allam, Bassem Seawater carbonate chemistry and phenotypic Plasticity in Crassostrea virginica Larvae |
topic_facet |
Alkalinity total standard deviation Animalia Aragonite saturation state Bicarbonate ion Calcite saturation state Calculated using seacarb Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Coast and continental shelf Containers and aquaria (20-1000 L or < 1 m**2) Coulometric titration Crassostrea virginica EXP Experiment Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Growth/Morphology Identification Laboratory experiment Length Mollusca Mortality Mortality/Survival Mount_Sinai_Harbor North Atlantic Northport_NY OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos |
description |
Ocean acidification (OA) is a major threat to marine calcifiers, and little is known regarding acclimation to OA in bivalves. This study combined physiological assays with next-generation sequencing to assess the potential for recovery from and acclimation to OA in the eastern oyster (Crassostrea virginica) and identify molecular mechanisms associated with resilience. In a reciprocal transplant experiment, larvae transplanted from elevated pCO2 (~1400 ppm) to ambient pCO2 (~350 ppm) demonstrated significantly lower mortality and larger size post-transplant than oysters remaining under elevated pCO2 and had similar mortality compared to those remaining in ambient conditions. The recovery after transplantation to ambient conditions demonstrates the ability for larvae to rebound and suggests phenotypic plasticity and acclimation. Transcriptomic analysis supported this hypothesis as genes were differentially regulated under OA stress. Transcriptomic profiles of transplanted and non-transplanted larvae terminating in the same final pCO2 converged, further supporting the idea that acclimation underlies resilience. The functions of differentially expressed genes included cell differentiation, development, biomineralization, ion exchange, and immunity. Results suggest acclimation as a mode of resilience to OA. In addition, the identification of genes associated with resilience can serve as a valuable resource for the aquaculture industry, as these could enable marker-assisted selection of OA-resilient stocks. |
format |
Dataset |
author |
Barbosa, Michelle Schwaner, Caroline Espinosa, Emmanuelle Pales Allam, Bassem |
author_facet |
Barbosa, Michelle Schwaner, Caroline Espinosa, Emmanuelle Pales Allam, Bassem |
author_sort |
Barbosa, Michelle |
title |
Seawater carbonate chemistry and phenotypic Plasticity in Crassostrea virginica Larvae |
title_short |
Seawater carbonate chemistry and phenotypic Plasticity in Crassostrea virginica Larvae |
title_full |
Seawater carbonate chemistry and phenotypic Plasticity in Crassostrea virginica Larvae |
title_fullStr |
Seawater carbonate chemistry and phenotypic Plasticity in Crassostrea virginica Larvae |
title_full_unstemmed |
Seawater carbonate chemistry and phenotypic Plasticity in Crassostrea virginica Larvae |
title_sort |
seawater carbonate chemistry and phenotypic plasticity in crassostrea virginica larvae |
publisher |
PANGAEA |
publishDate |
2023 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.957780 |
op_coverage |
MEDIAN LATITUDE: 41.168275 * MEDIAN LONGITUDE: -72.212275 * SOUTH-BOUND LATITUDE: 40.884000 * WEST-BOUND LONGITUDE: -73.343200 * NORTH-BOUND LATITUDE: 41.930500 * EAST-BOUND LONGITUDE: -70.031000 |
long_lat |
ENVELOPE(-73.343200,-70.031000,41.930500,40.884000) |
genre |
North Atlantic Ocean acidification |
genre_facet |
North Atlantic Ocean acidification |
op_relation |
Barbosa, Michelle; Schwaner, Caroline; Espinosa, Emmanuelle Pales; Allam, Bassem (2022): A Transcriptomic Analysis of Phenotypic Plasticity in Crassostrea virginica Larvae under Experimental Acidification. Genes, 13(9), 1529, https://doi.org/10.3390/genes13091529 Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2022): seacarb: seawater carbonate chemistry with R. R package version 3.3.1. https://cran.r-project.org/web/packages/seacarb/index.html https://doi.pangaea.de/10.1594/PANGAEA.957780 |
op_rights |
CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.3390/genes13091529 |
_version_ |
1768371630400077824 |