Seawater carbonate chemistry and cellular metabolism of the Arctic copepod Calanus glacialis

• Main Authors: Thor, Peter, Vermandele, Fanny, Bailey, Allison, Guscelli, Ella, Loubet‑Sartrou, Léa, Dupont, Sam, Calosi, Piero
• Format: Dataset
• Language: English
• Published: PANGAEA 2022
• Subjects: Alkalinity; total; standard deviation; Animalia; Aragonite saturation state; Arctic; Arthropoda; Bicarbonate ion; Bottles or small containers/Aquaria (<20 L); Calanus glacialis; Calcite saturation state; Calculated using seacarb after Nisumaa et al. (2010); Calculated using seacarb after Orr et al. (2018); Carbon; inorganic; dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Coast and continental shelf; EXP; Experiment; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Fugacity of carbon dioxide in seawater; Kongsfjord_copepod; Laboratory experiment; Metabolite; OA-ICC; Ocean Acidification International Coordination Centre; Other studied parameter or process; Partial pressure of carbon dioxide; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Arctic copepod; Kongsfjord*; Ocean acidification
• Online Access: https://doi.pangaea.de/10.1594/PANGAEA.957185; https://doi.org/10.1594/PANGAEA.957185

Using a targeted metabolomic approach we investigated the effects of low seawater pH on energy metabolism in two late copepodite stages (CIV and CV) of the keystone Arctic copepod species Calanus glacialis. Exposure to decreasing seawater pH (from 8.0 to 7.0) caused increased ATP, ADP and NAD+ and decreased AMP concentrations in stage CIV, and increased ATP and phospho-L-arginine and decreased AMP concentrations in stage CV. Metabolic pathway enrichment analysis showed enrichment of the TCA cycle and a range of amino acid metabolic pathways in both stages. Concentrations of lactate, malate, fumarate and alpha-ketoglutarate (all involved in the TCA cycle) increased in stage CIV, whereas only alpha-ketoglutarate increased in stage CV. Based on the pattern of concentration changes in glucose, pyruvate, TCA cycle metabolites, and free amino acids, we hypothesise that ocean acidification will lead to a shift in energy production from carbohydrate metabolism in the glycolysis toward amino acid metabolism in the TCA cycle and oxidative phosphorylation in stage CIV. In stage CV, concentrations of most of the analysed free fatty acids increased, suggesting in particular that ocean acidification increases the metabolism of stored wax esters in this stage. Moreover, aminoacyl-tRNA biosynthesis was enriched in both stages indicating increased enzyme production to handle low pH stress.