Seawater carbonate chemistry and cellular inorganic carbon fluxes in Trichodesmium

• Main Authors: Eichner, Meri, Thoms, Silke, Kranz, Sven A, Rost, Björn
• Format: Dataset
• Language: English
• Published: PANGAEA 2015
• Subjects: Alkalinity; total; standard deviation; Aragonite saturation state; Bacteria; Bicarbonate ion; Bicarbonate uptake in chlorophyll; Bicarbonate uptake rate in chlorophyll; Bottles or small containers/Aquaria (<20 L); Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon; inorganic; dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Carbon dioxide efflux; per chlorophyll a; Carbon dioxide uptake in chlorophyll; Carbon dioxide uptake rate in chlorophyll; Carbon fixation rate; Carbon uptake rate; Cyanobacteria; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gross carbon uptake rate; Laboratory experiment; Laboratory strains; Not applicable; OA-ICC; Ocean Acidification International Coordination Centre; Other metabolic rates; Partial pressure of carbon dioxide; Ocean acidification
• Online Access: https://doi.pangaea.de/10.1594/PANGAEA.956021; https://doi.org/10.1594/PANGAEA.956021

To predict effects of climate change on phytoplankton, it is crucial to understand how their mechanisms for carbon acquisition respond to environmental conditions. Aiming to shed light on the responses of extra- and intracellular inorganic C (Ci) fluxes, the cyanobacterium Trichodesmium erythraeum IMS101 was grown with different nitrogen sources (N2 vs NO3 –) and pCO2 levels (380 vs 1400 µatm). Cellular Ci fluxes were assessed by combining membrane inlet mass spectrometry (MIMS), 13C fractionation measurements, and modelling. Aside from a significant decrease in Ci affinity at elevated pCO2 and changes in CO2 efflux with different N sources, extracellular Ci fluxes estimated by MIMS were largely unaffected by the treatments. 13C fractionation during biomass production, however, increased with pCO2, irrespective of the N source. Strong discrepancies were observed in CO2 leakage estimates obtained by MIMS and a 13C-based approach, which further increased under elevated pCO2. These offsets could be explained by applying a model that comprises extracellular CO2 and HCO3– fluxes as well as internal Ci cycling around the carboxysome via the CO2 uptake facilitator NDH-14. Assuming unidirectional, kinetic fractionation between CO2 and HCO3– in the cytosol or enzymatic fractionation by NDH-14, both significantly improved the comparability of leakage estimates. Our results highlight the importance of internal Ci cycling for 13C composition as well as cellular energy budgets of Trichodesmium, which ought to be considered in process studies on climate change effects.