Seawater carbonate chemistry and Synechococcus growth from pCO2 experiments
Many microbial photoautotrophs depend on heterotrophic bacteria for accomplishing essential functions. Environmental changes, however, could alter or eliminate such interactions. We investigated the effects of changing pCO2 on gene transcription in co-cultures of 3 strains of picocyanobacteria (Syne...
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PANGAEA
2022
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Online Access: | https://doi.pangaea.de/10.1594/PANGAEA.955830 https://doi.org/10.1594/PANGAEA.955830 |
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.955830 |
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openpolar |
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Open Polar |
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PANGAEA - Data Publisher for Earth & Environmental Science |
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ftpangaea |
language |
English |
topic |
Alkalinity total Alteromonas sp. Aragonite saturation state Bacteria Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Cell density Cyanobacteria Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene expression (incl. proteomics) Growth/Morphology Heterotrophic prokaryotes Identification Laboratory experiment Laboratory strains Not applicable OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos pH standard deviation Phytoplankton Prochlorococcus sp. Proteobacteria Replicate Salinity Species Species interaction Strain Synechococcus sp. Temperature water Time in days Treatment Type |
spellingShingle |
Alkalinity total Alteromonas sp. Aragonite saturation state Bacteria Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Cell density Cyanobacteria Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene expression (incl. proteomics) Growth/Morphology Heterotrophic prokaryotes Identification Laboratory experiment Laboratory strains Not applicable OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos pH standard deviation Phytoplankton Prochlorococcus sp. Proteobacteria Replicate Salinity Species Species interaction Strain Synechococcus sp. Temperature water Time in days Treatment Type Barreto Filho, Marcelo Malisano Lu, Zhiying Walker, Melissa Morris, J Jeffrey Seawater carbonate chemistry and Synechococcus growth from pCO2 experiments |
topic_facet |
Alkalinity total Alteromonas sp. Aragonite saturation state Bacteria Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Cell density Cyanobacteria Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene expression (incl. proteomics) Growth/Morphology Heterotrophic prokaryotes Identification Laboratory experiment Laboratory strains Not applicable OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos pH standard deviation Phytoplankton Prochlorococcus sp. Proteobacteria Replicate Salinity Species Species interaction Strain Synechococcus sp. Temperature water Time in days Treatment Type |
description |
Many microbial photoautotrophs depend on heterotrophic bacteria for accomplishing essential functions. Environmental changes, however, could alter or eliminate such interactions. We investigated the effects of changing pCO2 on gene transcription in co-cultures of 3 strains of picocyanobacteria (Synechococcus strains CC9311 and WH8102 and Prochlorococcus strain MIT9312) paired with the 'helper' bacterium Alteromonas macleodii EZ55. Co-culture with cyanobacteria resulted in a much higher number of up- and down-regulated genes in EZ55 than pCO2 by itself. Pathway analysis revealed significantly different transcription of genes involved in carbohydrate metabolism, stress response, and chemotaxis, with different patterns of up- or down-regulation in co-culture with different cyanobacterial strains. Gene transcription patterns of organic and inorganic nutrient transporter and catabolism genes in EZ55 suggested resources available in the culture media were altered under elevated (800 ppm) pCO2 conditions. Altogether, changing transcription patterns were consistent with the possibility that the composition of cyanobacterial excretions changed under the two pCO2 regimes, causing extensive ecophysiological changes in both members of the co-cultures. Additionally, significant downregulation of oxidative stress genes in MIT9312/EZ55 cocultures at 800 ppm pCO2 were consistent with a link between the predicted reduced availability of photorespiratory byproducts (i.e., glycolate/2PG) under this condition and observed reductions in internal oxidative stress loads for EZ55, providing a possible explanation for the previously observed lack of “help” provided by EZ55 to MIT9312 under elevated pCO2. If similar broad alterations in microbial ecophysiology occur in the ocean as atmospheric pCO2 increases, they could lead to substantially altered ecosystem functioning and community composition. |
format |
Dataset |
author |
Barreto Filho, Marcelo Malisano Lu, Zhiying Walker, Melissa Morris, J Jeffrey |
author_facet |
Barreto Filho, Marcelo Malisano Lu, Zhiying Walker, Melissa Morris, J Jeffrey |
author_sort |
Barreto Filho, Marcelo Malisano |
title |
Seawater carbonate chemistry and Synechococcus growth from pCO2 experiments |
title_short |
Seawater carbonate chemistry and Synechococcus growth from pCO2 experiments |
title_full |
Seawater carbonate chemistry and Synechococcus growth from pCO2 experiments |
title_fullStr |
Seawater carbonate chemistry and Synechococcus growth from pCO2 experiments |
title_full_unstemmed |
Seawater carbonate chemistry and Synechococcus growth from pCO2 experiments |
title_sort |
seawater carbonate chemistry and synechococcus growth from pco2 experiments |
publisher |
PANGAEA |
publishDate |
2022 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.955830 https://doi.org/10.1594/PANGAEA.955830 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
Barreto Filho, Marcelo Malisano; Lu, Zhiying; Walker, Melissa; Morris, J Jeffrey (2022): Community context and pCO2 impact the transcriptome of the “helper” bacterium Alteromonas in co-culture with picocyanobacteria. ISME Communications, 2(1), 113, https://doi.org/10.1038/s43705-022-00197-2 Morris, J Jeffrey (2022): Synechococcus (WH8102 and CC9311) growth and genetic sequence accessions from experiments with variable pCO2 treatments from 2016 to 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO), https://doi.org/10.26008/1912/bco-dmo.882390.1 Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html https://doi.pangaea.de/10.1594/PANGAEA.955830 https://doi.org/10.1594/PANGAEA.955830 |
op_rights |
CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1594/PANGAEA.95583010.1038/s43705-022-00197-210.26008/1912/bco-dmo.882390.1 |
_version_ |
1810469819448819712 |
spelling |
ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.955830 2024-09-15T18:28:27+00:00 Seawater carbonate chemistry and Synechococcus growth from pCO2 experiments Barreto Filho, Marcelo Malisano Lu, Zhiying Walker, Melissa Morris, J Jeffrey 2022 text/tab-separated-values, 16031 data points https://doi.pangaea.de/10.1594/PANGAEA.955830 https://doi.org/10.1594/PANGAEA.955830 en eng PANGAEA Barreto Filho, Marcelo Malisano; Lu, Zhiying; Walker, Melissa; Morris, J Jeffrey (2022): Community context and pCO2 impact the transcriptome of the “helper” bacterium Alteromonas in co-culture with picocyanobacteria. ISME Communications, 2(1), 113, https://doi.org/10.1038/s43705-022-00197-2 Morris, J Jeffrey (2022): Synechococcus (WH8102 and CC9311) growth and genetic sequence accessions from experiments with variable pCO2 treatments from 2016 to 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO), https://doi.org/10.26008/1912/bco-dmo.882390.1 Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html https://doi.pangaea.de/10.1594/PANGAEA.955830 https://doi.org/10.1594/PANGAEA.955830 CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess Alkalinity total Alteromonas sp. Aragonite saturation state Bacteria Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Cell density Cyanobacteria Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene expression (incl. proteomics) Growth/Morphology Heterotrophic prokaryotes Identification Laboratory experiment Laboratory strains Not applicable OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos pH standard deviation Phytoplankton Prochlorococcus sp. Proteobacteria Replicate Salinity Species Species interaction Strain Synechococcus sp. Temperature water Time in days Treatment Type dataset 2022 ftpangaea https://doi.org/10.1594/PANGAEA.95583010.1038/s43705-022-00197-210.26008/1912/bco-dmo.882390.1 2024-07-24T02:31:35Z Many microbial photoautotrophs depend on heterotrophic bacteria for accomplishing essential functions. Environmental changes, however, could alter or eliminate such interactions. We investigated the effects of changing pCO2 on gene transcription in co-cultures of 3 strains of picocyanobacteria (Synechococcus strains CC9311 and WH8102 and Prochlorococcus strain MIT9312) paired with the 'helper' bacterium Alteromonas macleodii EZ55. Co-culture with cyanobacteria resulted in a much higher number of up- and down-regulated genes in EZ55 than pCO2 by itself. Pathway analysis revealed significantly different transcription of genes involved in carbohydrate metabolism, stress response, and chemotaxis, with different patterns of up- or down-regulation in co-culture with different cyanobacterial strains. Gene transcription patterns of organic and inorganic nutrient transporter and catabolism genes in EZ55 suggested resources available in the culture media were altered under elevated (800 ppm) pCO2 conditions. Altogether, changing transcription patterns were consistent with the possibility that the composition of cyanobacterial excretions changed under the two pCO2 regimes, causing extensive ecophysiological changes in both members of the co-cultures. Additionally, significant downregulation of oxidative stress genes in MIT9312/EZ55 cocultures at 800 ppm pCO2 were consistent with a link between the predicted reduced availability of photorespiratory byproducts (i.e., glycolate/2PG) under this condition and observed reductions in internal oxidative stress loads for EZ55, providing a possible explanation for the previously observed lack of “help” provided by EZ55 to MIT9312 under elevated pCO2. If similar broad alterations in microbial ecophysiology occur in the ocean as atmospheric pCO2 increases, they could lead to substantially altered ecosystem functioning and community composition. Dataset Ocean acidification PANGAEA - Data Publisher for Earth & Environmental Science |