Seawater carbonate chemistry and proton gradients across the coral calcifying cell layer
In corals, pH regulation of the extracellular calcifying medium (ECM) by the calcifying cell layer is a crucial step in the calcification process and is potentially important to influencing how corals respond to ocean acidification. Here, we analyzed the growing edge of the reef coral Stylophora pis...
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.953109 2023-05-15T17:50:14+02:00 Seawater carbonate chemistry and proton gradients across the coral calcifying cell layer Venn, Alexander A Tambutté, Eric Comeau, Steeve Tambutté, Sylvie 2022-12-23 text/tab-separated-values, 350 data points https://doi.pangaea.de/10.1594/PANGAEA.953109 en eng PANGAEA Venn, Alexander A; Tambutté, Eric; Comeau, Steeve; Tambutté, Sylvie (2022): Proton gradients across the coral calcifying cell layer: Effects of light, ocean acidification and carbonate chemistry. Frontiers in Marine Science, 9, 973908, https://doi.org/10.3389/fmars.2022.973908 Venn, Alexander A; Tambutté, Eric; Steeve, Comeau; Tambutté, Sylvie (2022): Proton gradients across the coral calcifying cell layer: effects of light, ocean acidification and carbonate chemistry. PANGAEA, https://doi.org/10.1594/PANGAEA.949400 Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html https://doi.pangaea.de/10.1594/PANGAEA.953109 CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess CC-BY Acid-base regulation Alkalinity total Animalia Aragonite saturation state Benthic animals Benthos Bicarbonate ion Calcite saturation state Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Cnidaria Comment Confocal Microscope (Leica SP5) Containers and aquaria (20-1000 L or < 1 m**2) Experiment Experiment/study setup Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Laboratory experiment Laboratory strains Not applicable OA-ICC Ocean Acidification International Coordination Centre Other Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) pH extracellular standard deviation intracellular mesoglea seawater Dataset 2022 ftpangaea https://doi.org/10.3389/fmars.2022.973908 https://doi.org/10.1594/PANGAEA.949400 2023-01-20T09:16:39Z In corals, pH regulation of the extracellular calcifying medium (ECM) by the calcifying cell layer is a crucial step in the calcification process and is potentially important to influencing how corals respond to ocean acidification. Here, we analyzed the growing edge of the reef coral Stylophora pistillata to make the first characterization of the proton gradient across the coral calcifying epithelium. At seawater pH 8 we found that while the calcifying epithelium elevates pH in the ECM on its apical side above that of seawater, pH on its basal side in the mesoglea is markedly lower, highlighting that the calcifying cells are exposed to a microenvironment distinct from the external environment. Coral symbiont photosynthesis elevates pH in the mesoglea, but experimental ocean acidification and decreased seawater inorganic carbon concentration lead to large declines in mesoglea pH relative to the ECM, which is maintained relatively stable. Together, our results indicate that the coral calcifying epithelium is functionally polarized and that environmental variation impacts pHECM regulation through its effects on the basal side of the calcifying cells. Dataset Ocean acidification PANGAEA - Data Publisher for Earth & Environmental Science |
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Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
Acid-base regulation Alkalinity total Animalia Aragonite saturation state Benthic animals Benthos Bicarbonate ion Calcite saturation state Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Cnidaria Comment Confocal Microscope (Leica SP5) Containers and aquaria (20-1000 L or < 1 m**2) Experiment Experiment/study setup Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Laboratory experiment Laboratory strains Not applicable OA-ICC Ocean Acidification International Coordination Centre Other Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) pH extracellular standard deviation intracellular mesoglea seawater |
spellingShingle |
Acid-base regulation Alkalinity total Animalia Aragonite saturation state Benthic animals Benthos Bicarbonate ion Calcite saturation state Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Cnidaria Comment Confocal Microscope (Leica SP5) Containers and aquaria (20-1000 L or < 1 m**2) Experiment Experiment/study setup Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Laboratory experiment Laboratory strains Not applicable OA-ICC Ocean Acidification International Coordination Centre Other Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) pH extracellular standard deviation intracellular mesoglea seawater Venn, Alexander A Tambutté, Eric Comeau, Steeve Tambutté, Sylvie Seawater carbonate chemistry and proton gradients across the coral calcifying cell layer |
topic_facet |
Acid-base regulation Alkalinity total Animalia Aragonite saturation state Benthic animals Benthos Bicarbonate ion Calcite saturation state Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Cnidaria Comment Confocal Microscope (Leica SP5) Containers and aquaria (20-1000 L or < 1 m**2) Experiment Experiment/study setup Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Laboratory experiment Laboratory strains Not applicable OA-ICC Ocean Acidification International Coordination Centre Other Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) pH extracellular standard deviation intracellular mesoglea seawater |
description |
In corals, pH regulation of the extracellular calcifying medium (ECM) by the calcifying cell layer is a crucial step in the calcification process and is potentially important to influencing how corals respond to ocean acidification. Here, we analyzed the growing edge of the reef coral Stylophora pistillata to make the first characterization of the proton gradient across the coral calcifying epithelium. At seawater pH 8 we found that while the calcifying epithelium elevates pH in the ECM on its apical side above that of seawater, pH on its basal side in the mesoglea is markedly lower, highlighting that the calcifying cells are exposed to a microenvironment distinct from the external environment. Coral symbiont photosynthesis elevates pH in the mesoglea, but experimental ocean acidification and decreased seawater inorganic carbon concentration lead to large declines in mesoglea pH relative to the ECM, which is maintained relatively stable. Together, our results indicate that the coral calcifying epithelium is functionally polarized and that environmental variation impacts pHECM regulation through its effects on the basal side of the calcifying cells. |
format |
Dataset |
author |
Venn, Alexander A Tambutté, Eric Comeau, Steeve Tambutté, Sylvie |
author_facet |
Venn, Alexander A Tambutté, Eric Comeau, Steeve Tambutté, Sylvie |
author_sort |
Venn, Alexander A |
title |
Seawater carbonate chemistry and proton gradients across the coral calcifying cell layer |
title_short |
Seawater carbonate chemistry and proton gradients across the coral calcifying cell layer |
title_full |
Seawater carbonate chemistry and proton gradients across the coral calcifying cell layer |
title_fullStr |
Seawater carbonate chemistry and proton gradients across the coral calcifying cell layer |
title_full_unstemmed |
Seawater carbonate chemistry and proton gradients across the coral calcifying cell layer |
title_sort |
seawater carbonate chemistry and proton gradients across the coral calcifying cell layer |
publisher |
PANGAEA |
publishDate |
2022 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.953109 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
Venn, Alexander A; Tambutté, Eric; Comeau, Steeve; Tambutté, Sylvie (2022): Proton gradients across the coral calcifying cell layer: Effects of light, ocean acidification and carbonate chemistry. Frontiers in Marine Science, 9, 973908, https://doi.org/10.3389/fmars.2022.973908 Venn, Alexander A; Tambutté, Eric; Steeve, Comeau; Tambutté, Sylvie (2022): Proton gradients across the coral calcifying cell layer: effects of light, ocean acidification and carbonate chemistry. PANGAEA, https://doi.org/10.1594/PANGAEA.949400 Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html https://doi.pangaea.de/10.1594/PANGAEA.953109 |
op_rights |
CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmars.2022.973908 https://doi.org/10.1594/PANGAEA.949400 |
_version_ |
1766156898919251968 |