Water record of a two factor (pH and infection) orthogonal experiment in wild Hong Kong oyster population
The basic observed water characteristics (pH, temperature, salinity and total alkalinity (TA)) and calculated water carbonate chemistry in the field (22.44°N, 113.95°E, Pak Nai, Hong Kong) and laboratory (22.28°N, 114.14°E, the University of Hong Kong, Hong Kong) during a two-factor orthogonal exper...
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.949588 2024-09-15T18:01:42+00:00 Water record of a two factor (pH and infection) orthogonal experiment in wild Hong Kong oyster population Dang, Xin MEDIAN LATITUDE: 22.360000 * MEDIAN LONGITUDE: 114.045000 * SOUTH-BOUND LATITUDE: 22.280000 * WEST-BOUND LONGITUDE: 113.950000 * NORTH-BOUND LATITUDE: 22.440000 * EAST-BOUND LONGITUDE: 114.140000 * DATE/TIME START: 2021-03-25T00:00:00 * DATE/TIME END: 2021-05-10T00:00:00 2022 text/tab-separated-values, 921 data points https://doi.pangaea.de/10.1594/PANGAEA.949588 https://doi.org/10.1594/PANGAEA.949588 en eng PANGAEA Dang, Xin; Lee, Tin Hang; Thiyagarajan, Vengatesen (2023): Wild oyster population resistance to ocean acidification adversely affected by bacterial infection. Environmental Pollution, 317, 120813, https://doi.org/10.1016/j.envpol.2022.120813 https://doi.pangaea.de/10.1594/PANGAEA.949588 https://doi.org/10.1594/PANGAEA.949588 CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess Alkalinity total Alkalinity titrator (Mettler Toledo G20S Compact Titrator Switzerland) Aragonite saturation state Calcite saturation state Calculated using CO2SYS Carbonate ion Carbon dioxide partial pressure DATE/TIME Dual-scale refractometer (MingRui China) Event label experiment Experimental treatment foodborne disease control Hand picking Hong Kong Region of the People's Republic of China Hong Kong oyster immune tolerance Laboratory experiment Local adaptation Pak_Nai_Experiment Pak_Nai_Field pathogen pH pH meter (Mettler Toledo InLab Semi-Micro) Salinity Temperature water wild population dataset 2022 ftpangaea https://doi.org/10.1594/PANGAEA.94958810.1016/j.envpol.2022.120813 2024-07-24T02:31:34Z The basic observed water characteristics (pH, temperature, salinity and total alkalinity (TA)) and calculated water carbonate chemistry in the field (22.44°N, 113.95°E, Pak Nai, Hong Kong) and laboratory (22.28°N, 114.14°E, the University of Hong Kong, Hong Kong) during a two-factor orthogonal experiment. To be specific, we designed a two-factor (low pH, as a proxy for OA, and Vibrio infection) orthogonal experiment to examine the immunological and molecular responses in the ecologically and economically important edible Hong Kong oyster, Crassostrea hongkongensis. Wild oyster population was collected from their native coastal area (22.44°N, 113.95°E, Pak Nai, Hong Kong). Vibrio parahaemolyticus, as a major global concern of foodborne human-related disease, was used in this study to investigate the antimicrobial characteristics of edible commercial oysters in the context of future OA. pH and temperature were monitored daily with pH meter (Mettler Toledo InLab®, Switzerland) calibrated with NBS standards. Salinity was monitored using a dual-scale refractometer (MingRui, China). The seawater was utterly changed every two days and 50 mL seawater was preserved with HgCl2 every week for total alkalinity (TA) measurement. The TA was measured using an alkalinity titrator (Mettler Toledo G20S Compact Titrator, Switzerland), and pCO2, CO32-, saturation states of calcite and aragonite were calculated by CO2SYS program with equilibrium constants K2 and KSO4 (Millero, F. J., Graham, T. B., Huang, F., Bustos-Serrano, H., & Pierrot, D. (2006). Dissociation constants of carbonic acid in seawater as a function of salinity and temperature. Marine Chemistry, 100(1), 80-94. https://doi.org/10.1016/j.marchem.2005.12.001). Dataset Carbonic acid PANGAEA - Data Publisher for Earth & Environmental Science ENVELOPE(113.950000,114.140000,22.440000,22.280000) |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
Alkalinity total Alkalinity titrator (Mettler Toledo G20S Compact Titrator Switzerland) Aragonite saturation state Calcite saturation state Calculated using CO2SYS Carbonate ion Carbon dioxide partial pressure DATE/TIME Dual-scale refractometer (MingRui China) Event label experiment Experimental treatment foodborne disease control Hand picking Hong Kong Region of the People's Republic of China Hong Kong oyster immune tolerance Laboratory experiment Local adaptation Pak_Nai_Experiment Pak_Nai_Field pathogen pH pH meter (Mettler Toledo InLab Semi-Micro) Salinity Temperature water wild population |
spellingShingle |
Alkalinity total Alkalinity titrator (Mettler Toledo G20S Compact Titrator Switzerland) Aragonite saturation state Calcite saturation state Calculated using CO2SYS Carbonate ion Carbon dioxide partial pressure DATE/TIME Dual-scale refractometer (MingRui China) Event label experiment Experimental treatment foodborne disease control Hand picking Hong Kong Region of the People's Republic of China Hong Kong oyster immune tolerance Laboratory experiment Local adaptation Pak_Nai_Experiment Pak_Nai_Field pathogen pH pH meter (Mettler Toledo InLab Semi-Micro) Salinity Temperature water wild population Dang, Xin Water record of a two factor (pH and infection) orthogonal experiment in wild Hong Kong oyster population |
topic_facet |
Alkalinity total Alkalinity titrator (Mettler Toledo G20S Compact Titrator Switzerland) Aragonite saturation state Calcite saturation state Calculated using CO2SYS Carbonate ion Carbon dioxide partial pressure DATE/TIME Dual-scale refractometer (MingRui China) Event label experiment Experimental treatment foodborne disease control Hand picking Hong Kong Region of the People's Republic of China Hong Kong oyster immune tolerance Laboratory experiment Local adaptation Pak_Nai_Experiment Pak_Nai_Field pathogen pH pH meter (Mettler Toledo InLab Semi-Micro) Salinity Temperature water wild population |
description |
The basic observed water characteristics (pH, temperature, salinity and total alkalinity (TA)) and calculated water carbonate chemistry in the field (22.44°N, 113.95°E, Pak Nai, Hong Kong) and laboratory (22.28°N, 114.14°E, the University of Hong Kong, Hong Kong) during a two-factor orthogonal experiment. To be specific, we designed a two-factor (low pH, as a proxy for OA, and Vibrio infection) orthogonal experiment to examine the immunological and molecular responses in the ecologically and economically important edible Hong Kong oyster, Crassostrea hongkongensis. Wild oyster population was collected from their native coastal area (22.44°N, 113.95°E, Pak Nai, Hong Kong). Vibrio parahaemolyticus, as a major global concern of foodborne human-related disease, was used in this study to investigate the antimicrobial characteristics of edible commercial oysters in the context of future OA. pH and temperature were monitored daily with pH meter (Mettler Toledo InLab®, Switzerland) calibrated with NBS standards. Salinity was monitored using a dual-scale refractometer (MingRui, China). The seawater was utterly changed every two days and 50 mL seawater was preserved with HgCl2 every week for total alkalinity (TA) measurement. The TA was measured using an alkalinity titrator (Mettler Toledo G20S Compact Titrator, Switzerland), and pCO2, CO32-, saturation states of calcite and aragonite were calculated by CO2SYS program with equilibrium constants K2 and KSO4 (Millero, F. J., Graham, T. B., Huang, F., Bustos-Serrano, H., & Pierrot, D. (2006). Dissociation constants of carbonic acid in seawater as a function of salinity and temperature. Marine Chemistry, 100(1), 80-94. https://doi.org/10.1016/j.marchem.2005.12.001). |
format |
Dataset |
author |
Dang, Xin |
author_facet |
Dang, Xin |
author_sort |
Dang, Xin |
title |
Water record of a two factor (pH and infection) orthogonal experiment in wild Hong Kong oyster population |
title_short |
Water record of a two factor (pH and infection) orthogonal experiment in wild Hong Kong oyster population |
title_full |
Water record of a two factor (pH and infection) orthogonal experiment in wild Hong Kong oyster population |
title_fullStr |
Water record of a two factor (pH and infection) orthogonal experiment in wild Hong Kong oyster population |
title_full_unstemmed |
Water record of a two factor (pH and infection) orthogonal experiment in wild Hong Kong oyster population |
title_sort |
water record of a two factor (ph and infection) orthogonal experiment in wild hong kong oyster population |
publisher |
PANGAEA |
publishDate |
2022 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.949588 https://doi.org/10.1594/PANGAEA.949588 |
op_coverage |
MEDIAN LATITUDE: 22.360000 * MEDIAN LONGITUDE: 114.045000 * SOUTH-BOUND LATITUDE: 22.280000 * WEST-BOUND LONGITUDE: 113.950000 * NORTH-BOUND LATITUDE: 22.440000 * EAST-BOUND LONGITUDE: 114.140000 * DATE/TIME START: 2021-03-25T00:00:00 * DATE/TIME END: 2021-05-10T00:00:00 |
long_lat |
ENVELOPE(113.950000,114.140000,22.440000,22.280000) |
genre |
Carbonic acid |
genre_facet |
Carbonic acid |
op_relation |
Dang, Xin; Lee, Tin Hang; Thiyagarajan, Vengatesen (2023): Wild oyster population resistance to ocean acidification adversely affected by bacterial infection. Environmental Pollution, 317, 120813, https://doi.org/10.1016/j.envpol.2022.120813 https://doi.pangaea.de/10.1594/PANGAEA.949588 https://doi.org/10.1594/PANGAEA.949588 |
op_rights |
CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1594/PANGAEA.94958810.1016/j.envpol.2022.120813 |
_version_ |
1810438796695568384 |