Seawater carbonate chemistry and survival rate of F2 Dicentrarchus labrax after viral challenge

• Main Authors: Cohen‐Rengifo, Mishal, Cabon, Joëlle, Danion, Morgane, Mazurais, David
• Format: Dataset
• Language: English
• Published: PANGAEA 2022
• Subjects: Alkalinity; total; standard error; Animalia; Aragonite saturation state; Bicarbonate ion; Calcite saturation state; Calculated using seacarb after Nisumaa et al. (2010); Carbon; inorganic; dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Chordata; Containers and aquaria (20-1000 L or < 1 m**2); Date; Dicentrarchus labrax; Fish; dead; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gene expression (incl. proteomics); Laboratory experiment; Laboratory strains; Mortality; Mortality/Survival; Nekton; Not applicable; OA-ICC; Ocean Acidification International Coordination Centre; Other; Other studied parameter or process; Oxygen; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH; Replicate; Salinity; Single species; Species; Ocean acidification
• Online Access: https://doi.pangaea.de/10.1594/PANGAEA.949153; https://doi.org/10.1594/PANGAEA.949153

Background: Progressive CO2-induced ocean acidification (OA) impacts marine life in ways that are difficult to predict but are likely to become exacerbated over generations. Although marine fishes can balance acid–base homeostasis efficiently, indirect ionic regulation that alter neurosensory systems can result in behavioural abnormalities. In marine invertebrates, OA can also affect immune system function, but whether this is the case in marine fishes is not fully understood. Farmed fish are highly susceptible to disease outbreak, yet strategies for overcoming such threats in the wake of OA are wanting. Here, we exposed two generations of the European sea bass (Dicentrarchus labrax) to end-of-century predicted pH levels (IPCC RCP8.5), with parents (F1) being exposed for four years and their offspring (F2) for 18 months. Our design included a transcriptomic analysis of the olfactory rosette (collected from the F2) and a viral challenge (exposing F2 to betanodavirus) where we assessed survival rates. Results: We discovered transcriptomic trade-offs in both sensory and immune systems after long-term transgenerational exposure to OA. Specifically, RNA-Seq analysis of the olfactory rosette, the peripheral olfactory organ, from 18-months-old F2 revealed extensive regulation in genes involved in ion transport and neuronal signalling, including GABAergic signalling. We also detected OA-induced up-regulation of genes associated with odour transduction, synaptic plasticity, neuron excitability and wiring and down-regulation of genes involved in energy metabolism. Furthermore, OA-exposure induced up-regulation of genes involved in innate antiviral immunity (pathogen recognition receptors and interferon-stimulated genes) in combination with down-regulation of the protein biosynthetic machinery. Consistently, OA-exposed F2 challenged with betanodavirus, which causes damage to the nervous system of marine fish, had acquired improved resistance. Conclusion: F2 exposed to long-term transgenerational OA acclimation showed ...