Gene expression by the cold-water octocoral Dentomuricea aff. meteor during an ex-situ experiment testing the effects of mining-generated sediment plumes

We report the results of an aquaria-based experiment testing the effects of suspended particles generated during potential mining activities, on a common habitat-building coral species in the Azores, Dentomuricea aff. meteor. Coral fragments were maintained in 10-L aquaria and exposed to three exper...

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Bibliographic Details
Main Authors: Carreiro-Silva, Marina, Martins, Ines, Raimundo, Joana, Caetano, Miguel, Bettencourt, Raul, Cerqueira, Teresa, Colaço, Ana
Format: Dataset
Language:English
Published: PANGAEA 2022
Subjects:
ATLAS
A Trans-Atlantic assessment and deep-water ecosystem-based spatial management plan for Europe
Cnidaria
Condor Seamounts
Cycles
enzyme activity
experiment
Experimental treatment
Experiment duration
gene expression
fold change
relative
standard error
Gene name
iAtlantic
Integrated Assessment of Atlantic Marine Ecosystems in Space and Time
JPI Oceans - Ecological Aspects of Deep-Sea Mining
JPIO-MiningImpact
Managing Impacts of Deep-seA reSource exploitation
metabolism
metal bioaccumulation
MIDAS
North Atlantic
octocorals
Real-Time PCR
CFX Connect™ Real-Time PCR Detection System (BioRad)
Sampling_Azores_Cold_Water_Octocoral
Specimen identification
Online Access:https://doi.pangaea.de/10.1594/PANGAEA.948413
https://doi.org/10.1594/PANGAEA.948413
Description
Summary:We report the results of an aquaria-based experiment testing the effects of suspended particles generated during potential mining activities, on a common habitat-building coral species in the Azores, Dentomuricea aff. meteor. Coral fragments were maintained in 10-L aquaria and exposed to three experimental treatments for a period of four weeks at the DeepSeaLab aquaria facilities (Okeanos-University of the Azores): (1) control conditions (no added sediments); (2) suspended polymetallic sulphide (PMS) particles; (3) suspended quartz particles. Gene expression profiles in D. aff. meteor were used to evaluate the physiological pathways involved in the response to exposure to PMS and quartz particles. Coral fragments were collected from each treatment at times 0, 3, and 13 days and for the control and quartz treatments also at time 27 days. The study targeted genes involved in cellular stress and antioxidant reaction system (heat shock protein, superoxide dismutase, ferritin), cell structure/integrity (α-carbonic anhydrase, receptor-type protein tyrosine phosphatase) and immune responses (toll-like receptor, lysozyme, rel homology domain, ferritin).

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