Cuticle thickness of juvenile California spiny lobsters (Panulirus interruptus) in response to ocean acidification
We examined the response of multiple structures used for predator defense in the California spiny lobster, Panulirus interruptus, to a series of ocean acidification-like conditions. Lobsters were collected by modified commercial traps offshore La Jolla, CA (in the area around 32.8534193, -117.268751...
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Online Access: | https://doi.pangaea.de/10.1594/PANGAEA.945356 https://doi.org/10.1594/PANGAEA.945356 |
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.945356 2024-09-15T18:27:55+00:00 Cuticle thickness of juvenile California spiny lobsters (Panulirus interruptus) in response to ocean acidification Lowder, Kaitlyn deVries, Maya S Hattingh, Ruan Day, James M D Andersson, Andreas J Zerofski, Phillip Taylor, Jennifer LATITUDE: 32.853419 * LONGITUDE: -117.268752 * DATE/TIME START: 2016-10-01T00:00:00 * DATE/TIME END: 2016-10-01T00:00:00 2022 text/tab-separated-values, 48882 data points https://doi.pangaea.de/10.1594/PANGAEA.945356 https://doi.org/10.1594/PANGAEA.945356 en eng PANGAEA https://doi.org/10.1594/PANGAEA.945362 Lowder, Kaitlyn; deVries, Maya S; Hattingh, Ruan; Day, James M D; Andersson, Andreas J; Zerofski, Phillip; Taylor, Jennifer (2022): Exoskeletal predator defenses of juvenile California spiny lobsters (Panulirus interruptus) are affected by fluctuating ocean acidification-like conditions. Frontiers in Marine Science, 9, 909017, https://doi.org/10.3389/fmars.2022.909017 Lowder, Kaitlyn (2022): kblowder/Spiny-lobster-exoskeleton-OA: code only. Zenodo, https://doi.org/10.5281/zenodo.6596558 https://doi.pangaea.de/10.1594/PANGAEA.945356 https://doi.org/10.1594/PANGAEA.945356 CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess Body region California Current Ecosystem crustacean Cuticle layer Distance Identification La_Jolla_trap_2016 Laboratory experiment Ocean acidification off Southern California pH standard deviation Quality control Scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX) Species unique identification unique identification (Semantic URI) unique identification (URI) Specimen identification Status Trap baited TRAPB Treatment Treatment: pH Treatment: temperature Type of study Validation flag/comment dataset 2022 ftpangaea https://doi.org/10.1594/PANGAEA.94535610.1594/PANGAEA.94536210.3389/fmars.2022.90901710.5281/zenodo.6596558 2024-07-24T02:31:43Z We examined the response of multiple structures used for predator defense in the California spiny lobster, Panulirus interruptus, to a series of ocean acidification-like conditions. Lobsters were collected by modified commercial traps offshore La Jolla, CA (in the area around 32.8534193, -117.2687516) in October 2016 and held at ambient conditions (pH 7.97, 16.5°C) before exposure to stable or diurnally fluctuating reduced pH conditions established by bubbling CO2 and as measured using best practices (ambient pH/stable, 7.97, 16.5°C; reduced pH/stable 7.67, 16.6°C; reduced pH with low fluctuations, 7.67 ± 0.05, 16.4°C; reduced pH with high fluctuations, 7.67 ± 0.10, 16.4°C). After three months, we examined cuticle layer thickness (µm) using a scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX). Each cuticle sample was rinsed with deionized water and allowed to air dry. Samples were then freeze-fractured with liquid nitrogen and critical-point dried (AutoSamdri 815 Series A, Tousimis, Rockville, MD, USA) before being mounted on a 90-degree SEM tip and sputter-coated with iridium. Cross-sections of these cuticle samples were examined with ultra-high-resolution scanning electron microscopy under high vacuum (XL30 SFEG with Sirion column and Apreo LoVac, FEI, Hillsboro, OR, USA with Oxford X-MAX 80 EDS detector, Concord, MA, USA) at 10 or 20 kV. One to two samples each of the carapace spine and antenna from individual lobsters were imaged and measured for the total cuticle thickness (epicuticle, exocuticle, and endocuticle), as well as thickness of the individual exo- and endocuticle layers. Dataset Ocean acidification PANGAEA - Data Publisher for Earth & Environmental Science ENVELOPE(-117.268752,-117.268752,32.853419,32.853419) |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
Body region California Current Ecosystem crustacean Cuticle layer Distance Identification La_Jolla_trap_2016 Laboratory experiment Ocean acidification off Southern California pH standard deviation Quality control Scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX) Species unique identification unique identification (Semantic URI) unique identification (URI) Specimen identification Status Trap baited TRAPB Treatment Treatment: pH Treatment: temperature Type of study Validation flag/comment |
spellingShingle |
Body region California Current Ecosystem crustacean Cuticle layer Distance Identification La_Jolla_trap_2016 Laboratory experiment Ocean acidification off Southern California pH standard deviation Quality control Scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX) Species unique identification unique identification (Semantic URI) unique identification (URI) Specimen identification Status Trap baited TRAPB Treatment Treatment: pH Treatment: temperature Type of study Validation flag/comment Lowder, Kaitlyn deVries, Maya S Hattingh, Ruan Day, James M D Andersson, Andreas J Zerofski, Phillip Taylor, Jennifer Cuticle thickness of juvenile California spiny lobsters (Panulirus interruptus) in response to ocean acidification |
topic_facet |
Body region California Current Ecosystem crustacean Cuticle layer Distance Identification La_Jolla_trap_2016 Laboratory experiment Ocean acidification off Southern California pH standard deviation Quality control Scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX) Species unique identification unique identification (Semantic URI) unique identification (URI) Specimen identification Status Trap baited TRAPB Treatment Treatment: pH Treatment: temperature Type of study Validation flag/comment |
description |
We examined the response of multiple structures used for predator defense in the California spiny lobster, Panulirus interruptus, to a series of ocean acidification-like conditions. Lobsters were collected by modified commercial traps offshore La Jolla, CA (in the area around 32.8534193, -117.2687516) in October 2016 and held at ambient conditions (pH 7.97, 16.5°C) before exposure to stable or diurnally fluctuating reduced pH conditions established by bubbling CO2 and as measured using best practices (ambient pH/stable, 7.97, 16.5°C; reduced pH/stable 7.67, 16.6°C; reduced pH with low fluctuations, 7.67 ± 0.05, 16.4°C; reduced pH with high fluctuations, 7.67 ± 0.10, 16.4°C). After three months, we examined cuticle layer thickness (µm) using a scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX). Each cuticle sample was rinsed with deionized water and allowed to air dry. Samples were then freeze-fractured with liquid nitrogen and critical-point dried (AutoSamdri 815 Series A, Tousimis, Rockville, MD, USA) before being mounted on a 90-degree SEM tip and sputter-coated with iridium. Cross-sections of these cuticle samples were examined with ultra-high-resolution scanning electron microscopy under high vacuum (XL30 SFEG with Sirion column and Apreo LoVac, FEI, Hillsboro, OR, USA with Oxford X-MAX 80 EDS detector, Concord, MA, USA) at 10 or 20 kV. One to two samples each of the carapace spine and antenna from individual lobsters were imaged and measured for the total cuticle thickness (epicuticle, exocuticle, and endocuticle), as well as thickness of the individual exo- and endocuticle layers. |
format |
Dataset |
author |
Lowder, Kaitlyn deVries, Maya S Hattingh, Ruan Day, James M D Andersson, Andreas J Zerofski, Phillip Taylor, Jennifer |
author_facet |
Lowder, Kaitlyn deVries, Maya S Hattingh, Ruan Day, James M D Andersson, Andreas J Zerofski, Phillip Taylor, Jennifer |
author_sort |
Lowder, Kaitlyn |
title |
Cuticle thickness of juvenile California spiny lobsters (Panulirus interruptus) in response to ocean acidification |
title_short |
Cuticle thickness of juvenile California spiny lobsters (Panulirus interruptus) in response to ocean acidification |
title_full |
Cuticle thickness of juvenile California spiny lobsters (Panulirus interruptus) in response to ocean acidification |
title_fullStr |
Cuticle thickness of juvenile California spiny lobsters (Panulirus interruptus) in response to ocean acidification |
title_full_unstemmed |
Cuticle thickness of juvenile California spiny lobsters (Panulirus interruptus) in response to ocean acidification |
title_sort |
cuticle thickness of juvenile california spiny lobsters (panulirus interruptus) in response to ocean acidification |
publisher |
PANGAEA |
publishDate |
2022 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.945356 https://doi.org/10.1594/PANGAEA.945356 |
op_coverage |
LATITUDE: 32.853419 * LONGITUDE: -117.268752 * DATE/TIME START: 2016-10-01T00:00:00 * DATE/TIME END: 2016-10-01T00:00:00 |
long_lat |
ENVELOPE(-117.268752,-117.268752,32.853419,32.853419) |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
https://doi.org/10.1594/PANGAEA.945362 Lowder, Kaitlyn; deVries, Maya S; Hattingh, Ruan; Day, James M D; Andersson, Andreas J; Zerofski, Phillip; Taylor, Jennifer (2022): Exoskeletal predator defenses of juvenile California spiny lobsters (Panulirus interruptus) are affected by fluctuating ocean acidification-like conditions. Frontiers in Marine Science, 9, 909017, https://doi.org/10.3389/fmars.2022.909017 Lowder, Kaitlyn (2022): kblowder/Spiny-lobster-exoskeleton-OA: code only. Zenodo, https://doi.org/10.5281/zenodo.6596558 https://doi.pangaea.de/10.1594/PANGAEA.945356 https://doi.org/10.1594/PANGAEA.945356 |
op_rights |
CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1594/PANGAEA.94535610.1594/PANGAEA.94536210.3389/fmars.2022.90901710.5281/zenodo.6596558 |
_version_ |
1810469195313315840 |