Cuticle atomic weight composition of juvenile California spiny lobsters (Panulirus interruptus) in response to ocean acidification
We examined the response of multiple structures used for predator defense in the California spiny lobster, Panulirus interruptus, to a series of ocean acidification-like conditions. Lobsters were collected by modified commercial traps offshore La Jolla, CA (in the area around 32.8534193, -117.268751...
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Language: | English |
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PANGAEA
2022
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Online Access: | https://doi.pangaea.de/10.1594/PANGAEA.945334 https://doi.org/10.1594/PANGAEA.945334 |
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.945334 |
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record_format |
openpolar |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
Aluminium Body region Calcium California Current Ecosystem Carbon Chlorine crustacean Cuticle layer Device type Identification La_Jolla_trap_2016 Laboratory experiment Magnesium Nitrogen Ocean acidification off Southern California Oxygen pH standard deviation Phosphorus Quality control Scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX) Silicon Sodium Species unique identification unique identification (Semantic URI) unique identification (URI) Specimen identification Status Sulfur Trap baited TRAPB Treatment Treatment: pH Treatment: temperature Type of study |
spellingShingle |
Aluminium Body region Calcium California Current Ecosystem Carbon Chlorine crustacean Cuticle layer Device type Identification La_Jolla_trap_2016 Laboratory experiment Magnesium Nitrogen Ocean acidification off Southern California Oxygen pH standard deviation Phosphorus Quality control Scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX) Silicon Sodium Species unique identification unique identification (Semantic URI) unique identification (URI) Specimen identification Status Sulfur Trap baited TRAPB Treatment Treatment: pH Treatment: temperature Type of study Lowder, Kaitlyn deVries, Maya S Hattingh, Ruan Day, James M D Andersson, Andreas J Zerofski, Phillip Taylor, Jennifer Cuticle atomic weight composition of juvenile California spiny lobsters (Panulirus interruptus) in response to ocean acidification |
topic_facet |
Aluminium Body region Calcium California Current Ecosystem Carbon Chlorine crustacean Cuticle layer Device type Identification La_Jolla_trap_2016 Laboratory experiment Magnesium Nitrogen Ocean acidification off Southern California Oxygen pH standard deviation Phosphorus Quality control Scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX) Silicon Sodium Species unique identification unique identification (Semantic URI) unique identification (URI) Specimen identification Status Sulfur Trap baited TRAPB Treatment Treatment: pH Treatment: temperature Type of study |
description |
We examined the response of multiple structures used for predator defense in the California spiny lobster, Panulirus interruptus, to a series of ocean acidification-like conditions. Lobsters were collected by modified commercial traps offshore La Jolla, CA (in the area around 32.8534193, -117.2687516) in October 2016 and held at ambient conditions (pH 7.97, 16.5°C) before exposure to stable or diurnally fluctuating reduced pH conditions established by bubbling CO2 and as measured using best practices (ambient pH/stable, 7.97, 16.5°C; reduced pH/stable 7.67, 16.6°C; reduced pH with low fluctuations, 7.67 ± 0.05, 16.4°C; reduced pH with high fluctuations, 7.67 ± 0.10, 16.4°C). After three months, we examined the atomic weight composition (%) of the carapace using a scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX). Each cuticle sample was rinsed with deionized water and allowed to air dry. Samples were then freeze-fractured with liquid nitrogen and critical-point dried (AutoSamdri 815 Series A, Tousimis, Rockville, MD, USA) before being mounted on a 90-degree SEM tip and sputter-coated with iridium. Cross-sections of these cuticle samples were examined with ultra-high-resolution scanning electron microscopy under high vacuum (XL30 SFEG with Sirion column and Apreo LoVac, FEI, Hillsboro, OR, USA with Oxford X-MAX 80 EDS detector, Concord, MA, USA) at 10 or 20 kV. One to two samples each of the carapace spine and antenna from individual lobsters were imaged. EDX was measured with with two machines (XL30 SFEG with Sirion column and Apreo LoVac, FEI, Hillsboro, OR, USA with Oxford X-MAX 80 EDS detector, Concord, MA, USA) at 20 kV acceleration voltage. Spectra were taken on the cross-sectional surface of the exocuticle and the endocuticle layers of the carapace spine and antenna base and the core and outer ring of the horn tip. |
format |
Dataset |
author |
Lowder, Kaitlyn deVries, Maya S Hattingh, Ruan Day, James M D Andersson, Andreas J Zerofski, Phillip Taylor, Jennifer |
author_facet |
Lowder, Kaitlyn deVries, Maya S Hattingh, Ruan Day, James M D Andersson, Andreas J Zerofski, Phillip Taylor, Jennifer |
author_sort |
Lowder, Kaitlyn |
title |
Cuticle atomic weight composition of juvenile California spiny lobsters (Panulirus interruptus) in response to ocean acidification |
title_short |
Cuticle atomic weight composition of juvenile California spiny lobsters (Panulirus interruptus) in response to ocean acidification |
title_full |
Cuticle atomic weight composition of juvenile California spiny lobsters (Panulirus interruptus) in response to ocean acidification |
title_fullStr |
Cuticle atomic weight composition of juvenile California spiny lobsters (Panulirus interruptus) in response to ocean acidification |
title_full_unstemmed |
Cuticle atomic weight composition of juvenile California spiny lobsters (Panulirus interruptus) in response to ocean acidification |
title_sort |
cuticle atomic weight composition of juvenile california spiny lobsters (panulirus interruptus) in response to ocean acidification |
publisher |
PANGAEA |
publishDate |
2022 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.945334 https://doi.org/10.1594/PANGAEA.945334 |
op_coverage |
LATITUDE: 32.853419 * LONGITUDE: -117.268752 * DATE/TIME START: 2016-10-01T00:00:00 * DATE/TIME END: 2016-10-01T00:00:00 |
long_lat |
ENVELOPE(-117.268752,-117.268752,32.853419,32.853419) |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
https://doi.org/10.1594/PANGAEA.945362 Lowder, Kaitlyn; deVries, Maya S; Hattingh, Ruan; Day, James M D; Andersson, Andreas J; Zerofski, Phillip; Taylor, Jennifer (2022): Exoskeletal predator defenses of juvenile California spiny lobsters (Panulirus interruptus) are affected by fluctuating ocean acidification-like conditions. Frontiers in Marine Science, 9, 909017, https://doi.org/10.3389/fmars.2022.909017 Lowder, Kaitlyn (2022): kblowder/Spiny-lobster-exoskeleton-OA: code only. Zenodo, https://doi.org/10.5281/zenodo.6596558 https://doi.pangaea.de/10.1594/PANGAEA.945334 https://doi.org/10.1594/PANGAEA.945334 |
op_rights |
CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1594/PANGAEA.94533410.1594/PANGAEA.94536210.3389/fmars.2022.90901710.5281/zenodo.6596558 |
_version_ |
1810469266250530816 |
spelling |
ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.945334 2024-09-15T18:27:58+00:00 Cuticle atomic weight composition of juvenile California spiny lobsters (Panulirus interruptus) in response to ocean acidification Lowder, Kaitlyn deVries, Maya S Hattingh, Ruan Day, James M D Andersson, Andreas J Zerofski, Phillip Taylor, Jennifer LATITUDE: 32.853419 * LONGITUDE: -117.268752 * DATE/TIME START: 2016-10-01T00:00:00 * DATE/TIME END: 2016-10-01T00:00:00 2022 text/tab-separated-values, 1876 data points https://doi.pangaea.de/10.1594/PANGAEA.945334 https://doi.org/10.1594/PANGAEA.945334 en eng PANGAEA https://doi.org/10.1594/PANGAEA.945362 Lowder, Kaitlyn; deVries, Maya S; Hattingh, Ruan; Day, James M D; Andersson, Andreas J; Zerofski, Phillip; Taylor, Jennifer (2022): Exoskeletal predator defenses of juvenile California spiny lobsters (Panulirus interruptus) are affected by fluctuating ocean acidification-like conditions. Frontiers in Marine Science, 9, 909017, https://doi.org/10.3389/fmars.2022.909017 Lowder, Kaitlyn (2022): kblowder/Spiny-lobster-exoskeleton-OA: code only. Zenodo, https://doi.org/10.5281/zenodo.6596558 https://doi.pangaea.de/10.1594/PANGAEA.945334 https://doi.org/10.1594/PANGAEA.945334 CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess Aluminium Body region Calcium California Current Ecosystem Carbon Chlorine crustacean Cuticle layer Device type Identification La_Jolla_trap_2016 Laboratory experiment Magnesium Nitrogen Ocean acidification off Southern California Oxygen pH standard deviation Phosphorus Quality control Scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX) Silicon Sodium Species unique identification unique identification (Semantic URI) unique identification (URI) Specimen identification Status Sulfur Trap baited TRAPB Treatment Treatment: pH Treatment: temperature Type of study dataset 2022 ftpangaea https://doi.org/10.1594/PANGAEA.94533410.1594/PANGAEA.94536210.3389/fmars.2022.90901710.5281/zenodo.6596558 2024-07-24T02:31:43Z We examined the response of multiple structures used for predator defense in the California spiny lobster, Panulirus interruptus, to a series of ocean acidification-like conditions. Lobsters were collected by modified commercial traps offshore La Jolla, CA (in the area around 32.8534193, -117.2687516) in October 2016 and held at ambient conditions (pH 7.97, 16.5°C) before exposure to stable or diurnally fluctuating reduced pH conditions established by bubbling CO2 and as measured using best practices (ambient pH/stable, 7.97, 16.5°C; reduced pH/stable 7.67, 16.6°C; reduced pH with low fluctuations, 7.67 ± 0.05, 16.4°C; reduced pH with high fluctuations, 7.67 ± 0.10, 16.4°C). After three months, we examined the atomic weight composition (%) of the carapace using a scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX). Each cuticle sample was rinsed with deionized water and allowed to air dry. Samples were then freeze-fractured with liquid nitrogen and critical-point dried (AutoSamdri 815 Series A, Tousimis, Rockville, MD, USA) before being mounted on a 90-degree SEM tip and sputter-coated with iridium. Cross-sections of these cuticle samples were examined with ultra-high-resolution scanning electron microscopy under high vacuum (XL30 SFEG with Sirion column and Apreo LoVac, FEI, Hillsboro, OR, USA with Oxford X-MAX 80 EDS detector, Concord, MA, USA) at 10 or 20 kV. One to two samples each of the carapace spine and antenna from individual lobsters were imaged. EDX was measured with with two machines (XL30 SFEG with Sirion column and Apreo LoVac, FEI, Hillsboro, OR, USA with Oxford X-MAX 80 EDS detector, Concord, MA, USA) at 20 kV acceleration voltage. Spectra were taken on the cross-sectional surface of the exocuticle and the endocuticle layers of the carapace spine and antenna base and the core and outer ring of the horn tip. Dataset Ocean acidification PANGAEA - Data Publisher for Earth & Environmental Science ENVELOPE(-117.268752,-117.268752,32.853419,32.853419) |