Seawater carbonate chemistry and the physiological responses of harmful dinoflagellate Karenia mikimotoi
The HAB-forming, toxic dinoflagellate Karenia mikimotoi, previously found to benefit from ocean acidification (OA), was cultivated to investigate its transcriptional response to simulated OA for 30 generations. Batch cultures were grown under two CO2 concentrations, 450 (control) and 1100 (simulated...
Main Authors: | , , , , , , , |
---|---|
Format: | Dataset |
Language: | English |
Published: |
PANGAEA
2022
|
Subjects: | |
Online Access: | https://doi.pangaea.de/10.1594/PANGAEA.945242 https://doi.org/10.1594/PANGAEA.945242 |
id |
ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.945242 |
---|---|
record_format |
openpolar |
spelling |
ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.945242 2023-05-15T17:51:12+02:00 Seawater carbonate chemistry and the physiological responses of harmful dinoflagellate Karenia mikimotoi Zhang, J Yang, Q Liu, Q Liu, S Zhu, Yue Yao, Jiang Wang, Hong Guan, WanChun 2022-06-09 text/tab-separated-values, 78 data points https://doi.pangaea.de/10.1594/PANGAEA.945242 https://doi.org/10.1594/PANGAEA.945242 en eng PANGAEA Zhang, J; Yang, Q; Liu, Q; Liu, S; Zhu, Yue; Yao, Jiang; Wang, Hong; Guan, WanChun (2022): The responses of harmful dinoflagellate Karenia mikimotoi to simulated ocean acidification at the transcriptional level. Harmful Algae, 111, 102167, https://doi.org/10.1016/j.hal.2021.102167 Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html https://doi.pangaea.de/10.1594/PANGAEA.945242 https://doi.org/10.1594/PANGAEA.945242 CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess CC-BY Alkalinity total Aragonite saturation state Bicarbonate ion standard deviation Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Carotenoids Carotenoids per cell Catalase activity unit per cell Chlorophyll a Chlorophyll a per cell Chromista Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Glutathione reductase activity Growth/Morphology Growth rate Karenia mikimotoi Laboratory experiment Laboratory strains Myzozoa Not applicable OA-ICC Ocean Acidification International Coordination Centre Dataset 2022 ftpangaea https://doi.org/10.1594/PANGAEA.945242 https://doi.org/10.1016/j.hal.2021.102167 2023-01-20T09:16:06Z The HAB-forming, toxic dinoflagellate Karenia mikimotoi, previously found to benefit from ocean acidification (OA), was cultivated to investigate its transcriptional response to simulated OA for 30 generations. Batch cultures were grown under two CO2 concentrations, 450 (control) and 1100 (simulated OA) μatm, and physiological parameters [growth, pigments, catalase (CAT), glutathione reductase (GR), and superoxide dismutase (SOD) activity], as well as transcriptomes (obtained via RNA-seq), were compared. Chlorophyll a (Chl a) and carotenoid (Caro) contents, as well as CAT and GR activities, were significantly increased under OA conditions. Transcriptomic analysis revealed 2,490 differentially expressed unigenes in response to OA, which comprised 1.54% of all unigenes. A total of 1,121 unigenes were upregulated, and 1,369 unigenes were downregulated in OA compared to control conditions. The downregulated expression of bicarbonate transporter and carbonic anhydrase genes was a landmark of OA acclimation. Key genes involved in energy metabolism, e.g., photosynthesis, tricarboxylic acid cycle, oxidative phosphorylation, and nitrogen metabolism, were highly upregulated under OA, contributing to increases in the Chl a (55.05%) and Caro (28.37%). The enhanced antioxidant enzyme activities (i.e. CAT, GR) and upregulated genes (i.e. glutathione peroxidase, ascorbate peroxidase, heat shock protein, 20S proteasome, aldehyde dehydrogenase, and apolipoprotein) benefit cells against the potential lower pH stress condition under OA. In addition, the downregulation of four genes associated with motility suggested that the preserved energy could further boost growth. In conclusion, the present study suggests that K. mikimotoi exhibits efficient gene expression regulation for the utilization of energy and resistance to OA-induced stress. Taken together, K. mikimotoi appeared as a tolerant species in response to OA. Thus, more extensive algal blooms that threaten marine organisms are likely in the future. These findings expand ... Dataset Ocean acidification PANGAEA - Data Publisher for Earth & Environmental Science |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
Alkalinity total Aragonite saturation state Bicarbonate ion standard deviation Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Carotenoids Carotenoids per cell Catalase activity unit per cell Chlorophyll a Chlorophyll a per cell Chromista Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Glutathione reductase activity Growth/Morphology Growth rate Karenia mikimotoi Laboratory experiment Laboratory strains Myzozoa Not applicable OA-ICC Ocean Acidification International Coordination Centre |
spellingShingle |
Alkalinity total Aragonite saturation state Bicarbonate ion standard deviation Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Carotenoids Carotenoids per cell Catalase activity unit per cell Chlorophyll a Chlorophyll a per cell Chromista Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Glutathione reductase activity Growth/Morphology Growth rate Karenia mikimotoi Laboratory experiment Laboratory strains Myzozoa Not applicable OA-ICC Ocean Acidification International Coordination Centre Zhang, J Yang, Q Liu, Q Liu, S Zhu, Yue Yao, Jiang Wang, Hong Guan, WanChun Seawater carbonate chemistry and the physiological responses of harmful dinoflagellate Karenia mikimotoi |
topic_facet |
Alkalinity total Aragonite saturation state Bicarbonate ion standard deviation Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Carotenoids Carotenoids per cell Catalase activity unit per cell Chlorophyll a Chlorophyll a per cell Chromista Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Glutathione reductase activity Growth/Morphology Growth rate Karenia mikimotoi Laboratory experiment Laboratory strains Myzozoa Not applicable OA-ICC Ocean Acidification International Coordination Centre |
description |
The HAB-forming, toxic dinoflagellate Karenia mikimotoi, previously found to benefit from ocean acidification (OA), was cultivated to investigate its transcriptional response to simulated OA for 30 generations. Batch cultures were grown under two CO2 concentrations, 450 (control) and 1100 (simulated OA) μatm, and physiological parameters [growth, pigments, catalase (CAT), glutathione reductase (GR), and superoxide dismutase (SOD) activity], as well as transcriptomes (obtained via RNA-seq), were compared. Chlorophyll a (Chl a) and carotenoid (Caro) contents, as well as CAT and GR activities, were significantly increased under OA conditions. Transcriptomic analysis revealed 2,490 differentially expressed unigenes in response to OA, which comprised 1.54% of all unigenes. A total of 1,121 unigenes were upregulated, and 1,369 unigenes were downregulated in OA compared to control conditions. The downregulated expression of bicarbonate transporter and carbonic anhydrase genes was a landmark of OA acclimation. Key genes involved in energy metabolism, e.g., photosynthesis, tricarboxylic acid cycle, oxidative phosphorylation, and nitrogen metabolism, were highly upregulated under OA, contributing to increases in the Chl a (55.05%) and Caro (28.37%). The enhanced antioxidant enzyme activities (i.e. CAT, GR) and upregulated genes (i.e. glutathione peroxidase, ascorbate peroxidase, heat shock protein, 20S proteasome, aldehyde dehydrogenase, and apolipoprotein) benefit cells against the potential lower pH stress condition under OA. In addition, the downregulation of four genes associated with motility suggested that the preserved energy could further boost growth. In conclusion, the present study suggests that K. mikimotoi exhibits efficient gene expression regulation for the utilization of energy and resistance to OA-induced stress. Taken together, K. mikimotoi appeared as a tolerant species in response to OA. Thus, more extensive algal blooms that threaten marine organisms are likely in the future. These findings expand ... |
format |
Dataset |
author |
Zhang, J Yang, Q Liu, Q Liu, S Zhu, Yue Yao, Jiang Wang, Hong Guan, WanChun |
author_facet |
Zhang, J Yang, Q Liu, Q Liu, S Zhu, Yue Yao, Jiang Wang, Hong Guan, WanChun |
author_sort |
Zhang, J |
title |
Seawater carbonate chemistry and the physiological responses of harmful dinoflagellate Karenia mikimotoi |
title_short |
Seawater carbonate chemistry and the physiological responses of harmful dinoflagellate Karenia mikimotoi |
title_full |
Seawater carbonate chemistry and the physiological responses of harmful dinoflagellate Karenia mikimotoi |
title_fullStr |
Seawater carbonate chemistry and the physiological responses of harmful dinoflagellate Karenia mikimotoi |
title_full_unstemmed |
Seawater carbonate chemistry and the physiological responses of harmful dinoflagellate Karenia mikimotoi |
title_sort |
seawater carbonate chemistry and the physiological responses of harmful dinoflagellate karenia mikimotoi |
publisher |
PANGAEA |
publishDate |
2022 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.945242 https://doi.org/10.1594/PANGAEA.945242 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
Zhang, J; Yang, Q; Liu, Q; Liu, S; Zhu, Yue; Yao, Jiang; Wang, Hong; Guan, WanChun (2022): The responses of harmful dinoflagellate Karenia mikimotoi to simulated ocean acidification at the transcriptional level. Harmful Algae, 111, 102167, https://doi.org/10.1016/j.hal.2021.102167 Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html https://doi.pangaea.de/10.1594/PANGAEA.945242 https://doi.org/10.1594/PANGAEA.945242 |
op_rights |
CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1594/PANGAEA.945242 https://doi.org/10.1016/j.hal.2021.102167 |
_version_ |
1766158266024329216 |