Seawater carbonate chemistry and blood and endolymph acid-base parameters in control and OA-exposed rockfish
Over a decade ago, ocean acidification (OA) exposure was reported to induce otolith overgrowth in teleost fish. This phenomenon was subsequently confirmed in multiple species; however, the underlying physiological causes remain unknown. Here, we report that splitnose rockfish (Sebastes diploproa) ex...
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.945127 2024-09-15T18:28:03+00:00 Seawater carbonate chemistry and blood and endolymph acid-base parameters in control and OA-exposed rockfish Kwan, Garfield Tsz Tresguerres, Martin 2022 text/tab-separated-values, 112 data points https://doi.pangaea.de/10.1594/PANGAEA.945127 https://doi.org/10.1594/PANGAEA.945127 en eng PANGAEA Kwan, Garfield Tsz; Tresguerres, Martin (2022): Elucidating the acid-base mechanisms underlying otolith overgrowth in fish exposed to ocean acidification. Science of the Total Environment, 823, 153690, https://doi.org/10.1016/j.scitotenv.2022.153690 Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html https://doi.pangaea.de/10.1594/PANGAEA.945127 https://doi.org/10.1594/PANGAEA.945127 CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess Acid-base regulation Alkalinity total standard error Animalia Aragonite saturation state Bicarbonate Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide partial pressure Chordata Coast and continental shelf Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Hydrogen ion concentration Laboratory experiment Nekton North Pacific OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos dataset 2022 ftpangaea https://doi.org/10.1594/PANGAEA.94512710.1016/j.scitotenv.2022.153690 2024-07-24T02:31:34Z Over a decade ago, ocean acidification (OA) exposure was reported to induce otolith overgrowth in teleost fish. This phenomenon was subsequently confirmed in multiple species; however, the underlying physiological causes remain unknown. Here, we report that splitnose rockfish (Sebastes diploproa) exposed to 1600 μatm pCO2 (pH 7.5) were able to fully regulated the pH of both blood and endolymph (the fluid that surrounds the otolith within the inner ear). However, while blood was regulated around pH 7.80, the endolymph was regulated around pH 8.30. These different pH setpoints result in increased pCO2 diffusion into the endolymph, which in turn leads to proportional increases in endolymph [HCO3−] and [CO32−]. Endolymph pH regulation despite the increased pCO2 suggests enhanced H+ removal. However, a lack of differences in inner ear bulk and cell-specific Na+/K+-ATPase and vacuolar type H+-ATPase protein abundance localization pointed out to activation of preexisting ATPases, non-bicarbonate pH buffering, or both, as the mechanism for endolymph pH-regulation. These results provide the first direct evidence showcasing the acid-base chemistry of the endolymph of OA-exposed fish favors otolith overgrowth, and suggests that this phenomenon will be more pronounced in species that count with more robust blood and endolymph pH regulatory mechanisms. Dataset Ocean acidification PANGAEA - Data Publisher for Earth & Environmental Science |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
Acid-base regulation Alkalinity total standard error Animalia Aragonite saturation state Bicarbonate Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide partial pressure Chordata Coast and continental shelf Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Hydrogen ion concentration Laboratory experiment Nekton North Pacific OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos |
spellingShingle |
Acid-base regulation Alkalinity total standard error Animalia Aragonite saturation state Bicarbonate Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide partial pressure Chordata Coast and continental shelf Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Hydrogen ion concentration Laboratory experiment Nekton North Pacific OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos Kwan, Garfield Tsz Tresguerres, Martin Seawater carbonate chemistry and blood and endolymph acid-base parameters in control and OA-exposed rockfish |
topic_facet |
Acid-base regulation Alkalinity total standard error Animalia Aragonite saturation state Bicarbonate Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide partial pressure Chordata Coast and continental shelf Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Hydrogen ion concentration Laboratory experiment Nekton North Pacific OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos |
description |
Over a decade ago, ocean acidification (OA) exposure was reported to induce otolith overgrowth in teleost fish. This phenomenon was subsequently confirmed in multiple species; however, the underlying physiological causes remain unknown. Here, we report that splitnose rockfish (Sebastes diploproa) exposed to 1600 μatm pCO2 (pH 7.5) were able to fully regulated the pH of both blood and endolymph (the fluid that surrounds the otolith within the inner ear). However, while blood was regulated around pH 7.80, the endolymph was regulated around pH 8.30. These different pH setpoints result in increased pCO2 diffusion into the endolymph, which in turn leads to proportional increases in endolymph [HCO3−] and [CO32−]. Endolymph pH regulation despite the increased pCO2 suggests enhanced H+ removal. However, a lack of differences in inner ear bulk and cell-specific Na+/K+-ATPase and vacuolar type H+-ATPase protein abundance localization pointed out to activation of preexisting ATPases, non-bicarbonate pH buffering, or both, as the mechanism for endolymph pH-regulation. These results provide the first direct evidence showcasing the acid-base chemistry of the endolymph of OA-exposed fish favors otolith overgrowth, and suggests that this phenomenon will be more pronounced in species that count with more robust blood and endolymph pH regulatory mechanisms. |
format |
Dataset |
author |
Kwan, Garfield Tsz Tresguerres, Martin |
author_facet |
Kwan, Garfield Tsz Tresguerres, Martin |
author_sort |
Kwan, Garfield Tsz |
title |
Seawater carbonate chemistry and blood and endolymph acid-base parameters in control and OA-exposed rockfish |
title_short |
Seawater carbonate chemistry and blood and endolymph acid-base parameters in control and OA-exposed rockfish |
title_full |
Seawater carbonate chemistry and blood and endolymph acid-base parameters in control and OA-exposed rockfish |
title_fullStr |
Seawater carbonate chemistry and blood and endolymph acid-base parameters in control and OA-exposed rockfish |
title_full_unstemmed |
Seawater carbonate chemistry and blood and endolymph acid-base parameters in control and OA-exposed rockfish |
title_sort |
seawater carbonate chemistry and blood and endolymph acid-base parameters in control and oa-exposed rockfish |
publisher |
PANGAEA |
publishDate |
2022 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.945127 https://doi.org/10.1594/PANGAEA.945127 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
Kwan, Garfield Tsz; Tresguerres, Martin (2022): Elucidating the acid-base mechanisms underlying otolith overgrowth in fish exposed to ocean acidification. Science of the Total Environment, 823, 153690, https://doi.org/10.1016/j.scitotenv.2022.153690 Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html https://doi.pangaea.de/10.1594/PANGAEA.945127 https://doi.org/10.1594/PANGAEA.945127 |
op_rights |
CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1594/PANGAEA.94512710.1016/j.scitotenv.2022.153690 |
_version_ |
1810469354195648512 |