Cell growth and Particulate Organic Carbon / Nitrogen content of Phaeocystis antarctica after exposure to different Fe and Mn availabilities at the end of the experiment

• Main Authors: Balaguer, Jenna, Thoms, Silke, Trimborn, Scarlett
• Format: Dataset
• Language: English
• Published: PANGAEA 2023
• Subjects: ANT-XXXIII/3; Carbon; organic; particulate; carbon fixation; Coulter counter; Beckman Coulter; Multisizer 3; Drake Passage; Element analyser Euro EA 3000; Experiment day; Fe:C ratio; growth; In situ pump; iron; ISP; manganese; Nitrogen; Phaeocystis antarctica; photophysiology; Phytoplankton; Polarstern; PS112; PS112_26-1; Southern Ocean; Trace metal; Treatment; Antarc*; Antarctica
• Online Access: https://doi.pangaea.de/10.1594/PANGAEA.944456; https://doi.org/10.1594/PANGAEA.944456

Cell counts and POC/PON content of P.antartica for the 4 different treatments were determined at the beginning and at the end of the experiment. The cell density and size were determined using a Beckman MultisizerTM 3 Coulter Counter® with a 100 μm aperture. For the POC/PON, 250 mL of water were filtered onto pre-combusted glass-fiber filters (15h, 500 ̊C, GF/F, ~0.6 μm, 25 mm, Whatman, Wisconsin, USA) and stored at -20°C in pre-combusted glass petri dishes. Prior to analysis with an Euro Elemental Analyzer 3000 CHNS-O (HEKAtech GmbH), the filters were dried for > 12h at 60 °C and then acidified with 200 μL of 0.2N HCL to remove inorganic carbon and dried a second time. Contents of POC and PON were corrected for blank measurements and normalized to filtered volume and cell densities to yield cellular quotas.