In-vitro hydrolysis rates of bio-based plastics under environmentally relevant temperatures

• Main Authors: Miksch, Lukas, Saborowski, Reinhard, Gutow, Lars
• Format: Dataset
• Language: English
• Published: PANGAEA 2022
• Subjects: Binary Object; Binary Object (File Size); bio-degradation; BIO-PLASTICS_EUROPE; Developing and Implementing Sustainability-Based Solutions for Bio-Based Plastic Production and Use to Preserve Land and Sea Environmental Quality in Europe; enzymes; File content; Hydrolysis; plastic; Polymer; temperature profiles; Use; Antarc*; Antarctica
• Online Access: https://doi.pangaea.de/10.1594/PANGAEA.943844; https://doi.org/10.1594/PANGAEA.943844

Bio-degradation assays were performed for bio-based plastics under environmentally relevant temperature between 5 and 30 °C using pH Stat titration. Suspensions of bio-based microparticles were incubated with different hydrolytic enzymes, a lipase from Candida antarctica, an esterase from Bacillus subtilis and a protease from Bacillus licheniformis. Rates of hydrolysis, as determined by counter-titration with a diluted base (NaOH), was recorded for two hours. The thermal profiles of plastic hydrolysis by all three enzymes were investigated. All measurements were conducted under controlled laboratory conditions.