Seawater carbonate chemistry and Arsenic accumulation of Crassostrea angulata and Crassostrea gigas

• Main Authors: Moreira, Anthony, Figueira, Etelvina, Mestre, Nélia C, Schrama, Denise, Soares, Amadeu M V M, Freitas, Rosa, Bebianno, Maria João
• Format: Dataset
• Language: English
• Published: PANGAEA 2018
• Subjects: Alkalinity; total; standard deviation; Animalia; Aragonite saturation state; Arsenic; Benthic animals; Benthos; Bicarbonate ion; Brackish waters; Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon; inorganic; dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Containers and aquaria (20-1000 L or < 1 m**2); Crassostrea angulata; Crassostrea gigas; Experiment duration; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Inorganic toxins; Laboratory experiment; Mollusca; North Atlantic; OA-ICC; Ocean Acidification International Coordination Centre; Other metabolic rates; Partial pressure of carbon dioxide; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH; Potentiometric; Ocean acidification
• Online Access: https://doi.pangaea.de/10.1594/PANGAEA.943191; https://doi.org/10.1594/PANGAEA.943191

Proteomic analysis was performed to compare the effects of Arsenic (As), seawater acidification (Low pH) and the combination of both stressors (Low pH + As) on Crassostrea angulata and Crassostrea gigas juveniles in the context of global environmental change. This study aimed to elucidate if two closely related Crassostrea species respond similarly to these environmental stressors, considering both single and combined exposures, to infer if the simultaneous exposure to both stressors induced a differentiated response. Identification of the most important differentially expressed proteins between conditions revealed marked differences in the response of each species towards single and combined exposures, evidencing species-related differences towards each experimental condition. Moreover, protein alterations observed in the combined exposure (Low pH + As) were substantially different from those observed in single exposures. Identified proteins and their putative biological functions revealed an array of modes of action in each condition. Among the most important, those involved in cellular structure (Actin, Atlastin, Severin, Gelsolin, Coronin) and extracellular matrix modulation (Ependymin, Tight junction ZO-1, Neprilysin) were strongly regulated, although in different exposure conditions and species. Data also revealed differences regarding metabolic modulation capacity (ATP beta, Enolase, Aconitate hydratase) and oxidative stress response (Aldehyde dehydrogenase, Lactoylglutathione, Retinal dehydrogenase) of the species, which also depended on single or combined exposures, illustrating a different response capacity of both oyster species to the presence of multiple stressors. Interestingly, alterations of piRNA abundance in C. angulata suggested genome reconfiguration in response to multiple stressors, likely an important mode of action related to adaptive evolution mechanisms previously unknown to oyster species, which requires further investigation. Our findings provide a deeper insight into the complexity ...