Enzyme activity after drying and bedform migration in the Spree River in 2018/2019

• Main Authors: Oprei, Anna, Schreckinger, José, Kholiavko, Tatiana, Frossard, Aline, Mutz, Michael, Risse-Buhl, Ute
• Format: Dataset
• Language: English
• Published: PANGAEA 2022
• Subjects: AMC; Infinite 200 fluorometer (Tecan; Männedorf; Switzerland); 365/450 nm (excitation/emmission wavelength); standardized to 20°C; Q10 factor of 2 (Winkler et al. 1996); Beta-glucosidase activity; Chitinase activity; DATE/TIME; drought; EcoMigRip; EXP; Experiment; L-DOPA; 200 fluorometer (Tecan; 365/460 nm (excitation/emmission wavelength); Leucine aminopeptidase activity; microbial abundance; microbial community; MUB; 365/445 nm (excitation/emmission wavelength); Overlooked bed shift? Modulation of the stream microbial food web and metabolism by patches of migrating sand ripples; Peroxidase activity; Phenol oxidase activity; Phosphatase activity; ripples; sediment transport; Spree_2018-2019; Spree River near Döbbrick; Brandenburg; Germany; streambed metabolism; Time in days; Treatment; Xylosidase activity; Arctic
• Online Access: https://doi.pangaea.de/10.1594/PANGAEA.941234

Sandy streambed sediments that had been exposed to either intense drying (90-day without rainfall) or bedform migration (periodically moving ripples) were placed in 250 µm mesh bags to the Spree River in Northeastern Germany on October 22, 2018, to initiate the recovery process from drying or migration stress. Subsamples from the recovering sediments were collected at eight dates within eight months and taken to the laboratory for further analysis. Potential extracellular enzyme activities (Beta-Glucosidase, Xylosidase, Chitinase, Phosphatase, Leucine-Aminopeptidase, Phenol-Oxidase, and Peroxidase) were assessed by measuring fluorescence and absorbance after extraction from sediments. The aim of the experiment was to observe the long-term recovery of microbial communities in lowland streams from drying and bedform migration and to identify if stress effects persisted as legacy after the stress period.