Community abundance after drying and bedform migration in the Spree River in 2018/2019

• Main Authors: Oprei, Anna, Schreckinger, José, Kholiavko, Tatiana, Frossard, Aline, Mutz, Michael, Risse-Buhl, Ute
• Format: Dataset
• Language: English
• Published: PANGAEA 2022
• Subjects: Chlorophyll a per unit sediment mass; Counting 400 living cells in Utermöhl chamber at 400x magnification; inverse light microscope (Axioskop2; Zeiss; Germany); extraction following Mendoza-Lera et al. (2017); DATE/TIME; Diatoms; drought; EcoMigRip; EXP; Experiment; Fungal abundance; ITS2 copy number per sediment dry mass; microbial abundance; microbial community; Overlooked bed shift? Modulation of the stream microbial food web and metabolism by patches of migrating sand ripples; Prokaryotic abundance; 16S gene copy number per sediment dry mass; qPCR (primer pairs 27F/519r) with SYBR™ Green in ABI7500 machine (Applied Biosystems; Foster City; USA); Perez-Mon et al. (2020); qPCR (primer pairs ITS3/ITS4) with SYBR™ Green in ABI7500 machine (Applied Biosystems; Reverse phase HPLC (High Performance Liquid Chromatography) using Ultimate3000 (Thermo Fisher Scientific Corporation; Waltham; MA; ripples; sediment transport; Spree_2018-2019; Spree River near Döbbrick; Brandenburg; Germany; streambed metabolism; Time in days; Treatment; Perez; Arctic
• Online Access: https://doi.pangaea.de/10.1594/PANGAEA.941189

Sandy streambed sediments that had been exposed to either intense drying (90-day without rainfall) or bedform migration (periodically moving ripples) were placed in 250 µm mesh bags to the Spree River in Northeastern Germany on October 22, 2018, to initiate the recovery process from drying or migration stress. Subsamples from the recovering sediments were collected at eight dates within eight months and taken to the laboratory for further analysis. Community abundance was assessed on a heterotrophic (amplified bacterial and fungal gene copies) and on an autotrophic level (Chlorophyll a concentration and diatom cell count). The aim of the experiment was to observe the long-term recovery of microbial communities in lowland streams from drying and bedform migration and to identify if stress effects persisted as legacy after the stress period.