Seawater carbonate chemistry and otop2l expression of sea urchins (Stongylocentrotus purpuratus)
Otopetrins comprise a family of proton-selective channels that are critically important for the mineralization of otoliths and statoconia in vertebrates but whose underlying cellular mechanisms remain largely unknown. Here, we demonstrate that otopetrins are critically involved in the calcification...
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2021
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.939436 2024-09-15T18:24:25+00:00 Seawater carbonate chemistry and otop2l expression of sea urchins (Stongylocentrotus purpuratus) Chang, William Weijen Matt, Ann-Sophie Schewe, Marcus Musinszki, Marianne Grüssel, Sandra Brandenburg, Jonas Garfield, David Bleich, Markus Baukrowitz, Thomas Hu, Marian Y 2021 text/tab-separated-values, 1125 data points https://doi.pangaea.de/10.1594/PANGAEA.939436 https://doi.org/10.1594/PANGAEA.939436 en eng PANGAEA Chang, William Weijen; Matt, Ann-Sophie; Schewe, Marcus; Musinszki, Marianne; Grüssel, Sandra; Brandenburg, Jonas; Garfield, David; Bleich, Markus; Baukrowitz, Thomas; Hu, Marian Y (2021): An otopetrin family proton channel promotes cellular acid efflux critical for biomineralization in a marine calcifier. Proceedings of the National Academy of Sciences, 118(30), e2101378118, https://doi.org/10.1073/pnas.2101378118 Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html https://doi.pangaea.de/10.1594/PANGAEA.939436 https://doi.org/10.1594/PANGAEA.939436 CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess Alkalinity total Animalia Aragonite saturation state Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Coast and continental shelf Echinodermata Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene expression Gene expression (incl. proteomics) Laboratory experiment North Atlantic Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos pH Registration number of species Replicate Salinity Single species Species Strongylocentrotus purpuratus Temperate Temperature water Time in days Treatment Type Uniform resource locator/link to reference Zooplankton dataset 2021 ftpangaea https://doi.org/10.1594/PANGAEA.93943610.1073/pnas.2101378118 2024-07-24T02:31:34Z Otopetrins comprise a family of proton-selective channels that are critically important for the mineralization of otoliths and statoconia in vertebrates but whose underlying cellular mechanisms remain largely unknown. Here, we demonstrate that otopetrins are critically involved in the calcification process by providing an exit route for protons liberated by the formation of CaCO3. Using the sea urchin larva, we examined the otopetrin ortholog otop2l, which is exclusively expressed in the calcifying primary mesenchymal cells (PMCs) that generate the calcitic larval skeleton. otop2l expression is stimulated during skeletogenesis, and knockdown of otop2l impairs spicule formation. Intracellular pH measurements demonstrated Zn2+-sensitive H+ fluxes in PMCs that regulate intracellular pH in a Na+/HCO3−-independent manner, while Otop2l knockdown reduced membrane proton permeability. Furthermore, Otop2l displays unique features, including strong activation by high extracellular pH (>8.0) and check-valve–like outwardly rectifying H+ flux properties, making it into a cellular proton extrusion machine adapted to oceanic living conditions. Our results provide evidence that otopetrin family proton channels are a central component of the cellular pH regulatory machinery in biomineralizing cells. Their ubiquitous occurrence in calcifying systems across the animal kingdom suggest a conserved physiological function by mediating pH at the site of mineralization. This important role of otopetrin family proton channels has strong implications for our view on the cellular mechanisms of biomineralization and their response to changes in oceanic pH. Dataset North Atlantic PANGAEA - Data Publisher for Earth & Environmental Science |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
Alkalinity total Animalia Aragonite saturation state Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Coast and continental shelf Echinodermata Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene expression Gene expression (incl. proteomics) Laboratory experiment North Atlantic Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos pH Registration number of species Replicate Salinity Single species Species Strongylocentrotus purpuratus Temperate Temperature water Time in days Treatment Type Uniform resource locator/link to reference Zooplankton |
spellingShingle |
Alkalinity total Animalia Aragonite saturation state Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Coast and continental shelf Echinodermata Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene expression Gene expression (incl. proteomics) Laboratory experiment North Atlantic Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos pH Registration number of species Replicate Salinity Single species Species Strongylocentrotus purpuratus Temperate Temperature water Time in days Treatment Type Uniform resource locator/link to reference Zooplankton Chang, William Weijen Matt, Ann-Sophie Schewe, Marcus Musinszki, Marianne Grüssel, Sandra Brandenburg, Jonas Garfield, David Bleich, Markus Baukrowitz, Thomas Hu, Marian Y Seawater carbonate chemistry and otop2l expression of sea urchins (Stongylocentrotus purpuratus) |
topic_facet |
Alkalinity total Animalia Aragonite saturation state Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Coast and continental shelf Echinodermata Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene expression Gene expression (incl. proteomics) Laboratory experiment North Atlantic Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos pH Registration number of species Replicate Salinity Single species Species Strongylocentrotus purpuratus Temperate Temperature water Time in days Treatment Type Uniform resource locator/link to reference Zooplankton |
description |
Otopetrins comprise a family of proton-selective channels that are critically important for the mineralization of otoliths and statoconia in vertebrates but whose underlying cellular mechanisms remain largely unknown. Here, we demonstrate that otopetrins are critically involved in the calcification process by providing an exit route for protons liberated by the formation of CaCO3. Using the sea urchin larva, we examined the otopetrin ortholog otop2l, which is exclusively expressed in the calcifying primary mesenchymal cells (PMCs) that generate the calcitic larval skeleton. otop2l expression is stimulated during skeletogenesis, and knockdown of otop2l impairs spicule formation. Intracellular pH measurements demonstrated Zn2+-sensitive H+ fluxes in PMCs that regulate intracellular pH in a Na+/HCO3−-independent manner, while Otop2l knockdown reduced membrane proton permeability. Furthermore, Otop2l displays unique features, including strong activation by high extracellular pH (>8.0) and check-valve–like outwardly rectifying H+ flux properties, making it into a cellular proton extrusion machine adapted to oceanic living conditions. Our results provide evidence that otopetrin family proton channels are a central component of the cellular pH regulatory machinery in biomineralizing cells. Their ubiquitous occurrence in calcifying systems across the animal kingdom suggest a conserved physiological function by mediating pH at the site of mineralization. This important role of otopetrin family proton channels has strong implications for our view on the cellular mechanisms of biomineralization and their response to changes in oceanic pH. |
format |
Dataset |
author |
Chang, William Weijen Matt, Ann-Sophie Schewe, Marcus Musinszki, Marianne Grüssel, Sandra Brandenburg, Jonas Garfield, David Bleich, Markus Baukrowitz, Thomas Hu, Marian Y |
author_facet |
Chang, William Weijen Matt, Ann-Sophie Schewe, Marcus Musinszki, Marianne Grüssel, Sandra Brandenburg, Jonas Garfield, David Bleich, Markus Baukrowitz, Thomas Hu, Marian Y |
author_sort |
Chang, William Weijen |
title |
Seawater carbonate chemistry and otop2l expression of sea urchins (Stongylocentrotus purpuratus) |
title_short |
Seawater carbonate chemistry and otop2l expression of sea urchins (Stongylocentrotus purpuratus) |
title_full |
Seawater carbonate chemistry and otop2l expression of sea urchins (Stongylocentrotus purpuratus) |
title_fullStr |
Seawater carbonate chemistry and otop2l expression of sea urchins (Stongylocentrotus purpuratus) |
title_full_unstemmed |
Seawater carbonate chemistry and otop2l expression of sea urchins (Stongylocentrotus purpuratus) |
title_sort |
seawater carbonate chemistry and otop2l expression of sea urchins (stongylocentrotus purpuratus) |
publisher |
PANGAEA |
publishDate |
2021 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.939436 https://doi.org/10.1594/PANGAEA.939436 |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_relation |
Chang, William Weijen; Matt, Ann-Sophie; Schewe, Marcus; Musinszki, Marianne; Grüssel, Sandra; Brandenburg, Jonas; Garfield, David; Bleich, Markus; Baukrowitz, Thomas; Hu, Marian Y (2021): An otopetrin family proton channel promotes cellular acid efflux critical for biomineralization in a marine calcifier. Proceedings of the National Academy of Sciences, 118(30), e2101378118, https://doi.org/10.1073/pnas.2101378118 Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html https://doi.pangaea.de/10.1594/PANGAEA.939436 https://doi.org/10.1594/PANGAEA.939436 |
op_rights |
CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1594/PANGAEA.93943610.1073/pnas.2101378118 |
_version_ |
1810464771240099840 |