Seawater carbonate chemistry and gene expression (RT-PCR) and enzyme activity of the Antarctic coral Malacobelemnon daytoni
Benthic organisms of the Southern Ocean are particularly vulnerable to ocean acidification (OA), as they inhabit cold waters where calcite-aragonite saturation states are naturally low. OA most strongly affects animals with calcium carbonate skeletons or shells, such as corals and mollusks. We expos...
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.936683 2024-09-15T17:42:41+00:00 Seawater carbonate chemistry and gene expression (RT-PCR) and enzyme activity of the Antarctic coral Malacobelemnon daytoni Servetto, Natalia de Aranzamendi, M C Bettencourt, Raul Held, Christoph Abele, Doris Movilla, Juancho González, G Bustos, D M Sahade, Ricardo José LATITUDE: -62.233300 * LONGITUDE: -58.666700 2021 text/tab-separated-values, 3461 data points https://doi.pangaea.de/10.1594/PANGAEA.936683 https://doi.org/10.1594/PANGAEA.936683 en eng PANGAEA Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html https://doi.pangaea.de/10.1594/PANGAEA.936683 https://doi.org/10.1594/PANGAEA.936683 CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess Alkalinity total standard deviation Animalia Antarctic Aragonite saturation state Benthic animals Benthos Bicarbonate ion Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Calculated using seacarb after Orr et al. (2018) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Catalase activity unit per protein mass Cnidaria Coast and continental shelf Containers and aquaria (20-1000 L or < 1 m**2) Day of experiment Dry air column-averaged mixing ratio of carbon dioxide EXP Experiment Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Fugacity of carbon dioxide in seawater Gene expression dataset 2021 ftpangaea https://doi.org/10.1594/PANGAEA.936683 2024-07-24T02:31:34Z Benthic organisms of the Southern Ocean are particularly vulnerable to ocean acidification (OA), as they inhabit cold waters where calcite-aragonite saturation states are naturally low. OA most strongly affects animals with calcium carbonate skeletons or shells, such as corals and mollusks. We exposed the abundant cold-water coral Malacobelemnon daytoni from an Antarctic fjord to low pH seawater (LpH) (7.68 +/- 0.17) to test its physiological responses to OA, at the level of gene expression (RT-PCR) and enzyme activity. Corals were exposed in short- (3 days) and long-term (54 days) experiments to two pCO2 conditions (ambient and elevated pCO2 equaling RCP 8.5, IPCC 2019, approximately 372.53 and 956.78 μatm, respectively). Of the eleven genes studied through RT-PCR, six were significantly upregulated compared with control in the short-term in the LpH condition, including the antioxidant enzyme superoxide dismutase (SOD), Heat Shock Protein 70 (HSP70), Toll-like receptor (TLR), galaxin and ferritin. After long-term exposure to low pH conditions, RT-PCR analysis showed seven genes were upregulated. These include the mannose-binding C-Lectin and HSP90. Also, the expression of TLR and galaxin, among others, continued to be upregulated after long-term exposure to low pH. Expression of carbonic anhydrase (CA), a key enzyme involved in calcification, was also significantly upregulated after long-term exposure. Our results indicated that, after two months, M. daytoni is not acclimatized to this experimental LpH condition. Gene expression profiles revealed molecular impacts that were not evident at the enzyme activity level. Consequently, understanding the molecular mechanisms behind the physiological processes in the response of a coral to LpH is critical to understanding the ability of polar species to cope with future environmental changes. Approaches integrating molecular tools into Antarctic ecological and/or conservation research make an essential contribution given the current ongoing OA processes. Dataset Antarc* Antarctic Ocean acidification Southern Ocean PANGAEA - Data Publisher for Earth & Environmental Science ENVELOPE(-58.666700,-58.666700,-62.233300,-62.233300) |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
Alkalinity total standard deviation Animalia Antarctic Aragonite saturation state Benthic animals Benthos Bicarbonate ion Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Calculated using seacarb after Orr et al. (2018) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Catalase activity unit per protein mass Cnidaria Coast and continental shelf Containers and aquaria (20-1000 L or < 1 m**2) Day of experiment Dry air column-averaged mixing ratio of carbon dioxide EXP Experiment Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Fugacity of carbon dioxide in seawater Gene expression |
spellingShingle |
Alkalinity total standard deviation Animalia Antarctic Aragonite saturation state Benthic animals Benthos Bicarbonate ion Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Calculated using seacarb after Orr et al. (2018) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Catalase activity unit per protein mass Cnidaria Coast and continental shelf Containers and aquaria (20-1000 L or < 1 m**2) Day of experiment Dry air column-averaged mixing ratio of carbon dioxide EXP Experiment Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Fugacity of carbon dioxide in seawater Gene expression Servetto, Natalia de Aranzamendi, M C Bettencourt, Raul Held, Christoph Abele, Doris Movilla, Juancho González, G Bustos, D M Sahade, Ricardo José Seawater carbonate chemistry and gene expression (RT-PCR) and enzyme activity of the Antarctic coral Malacobelemnon daytoni |
topic_facet |
Alkalinity total standard deviation Animalia Antarctic Aragonite saturation state Benthic animals Benthos Bicarbonate ion Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Calculated using seacarb after Orr et al. (2018) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Catalase activity unit per protein mass Cnidaria Coast and continental shelf Containers and aquaria (20-1000 L or < 1 m**2) Day of experiment Dry air column-averaged mixing ratio of carbon dioxide EXP Experiment Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Fugacity of carbon dioxide in seawater Gene expression |
description |
Benthic organisms of the Southern Ocean are particularly vulnerable to ocean acidification (OA), as they inhabit cold waters where calcite-aragonite saturation states are naturally low. OA most strongly affects animals with calcium carbonate skeletons or shells, such as corals and mollusks. We exposed the abundant cold-water coral Malacobelemnon daytoni from an Antarctic fjord to low pH seawater (LpH) (7.68 +/- 0.17) to test its physiological responses to OA, at the level of gene expression (RT-PCR) and enzyme activity. Corals were exposed in short- (3 days) and long-term (54 days) experiments to two pCO2 conditions (ambient and elevated pCO2 equaling RCP 8.5, IPCC 2019, approximately 372.53 and 956.78 μatm, respectively). Of the eleven genes studied through RT-PCR, six were significantly upregulated compared with control in the short-term in the LpH condition, including the antioxidant enzyme superoxide dismutase (SOD), Heat Shock Protein 70 (HSP70), Toll-like receptor (TLR), galaxin and ferritin. After long-term exposure to low pH conditions, RT-PCR analysis showed seven genes were upregulated. These include the mannose-binding C-Lectin and HSP90. Also, the expression of TLR and galaxin, among others, continued to be upregulated after long-term exposure to low pH. Expression of carbonic anhydrase (CA), a key enzyme involved in calcification, was also significantly upregulated after long-term exposure. Our results indicated that, after two months, M. daytoni is not acclimatized to this experimental LpH condition. Gene expression profiles revealed molecular impacts that were not evident at the enzyme activity level. Consequently, understanding the molecular mechanisms behind the physiological processes in the response of a coral to LpH is critical to understanding the ability of polar species to cope with future environmental changes. Approaches integrating molecular tools into Antarctic ecological and/or conservation research make an essential contribution given the current ongoing OA processes. |
format |
Dataset |
author |
Servetto, Natalia de Aranzamendi, M C Bettencourt, Raul Held, Christoph Abele, Doris Movilla, Juancho González, G Bustos, D M Sahade, Ricardo José |
author_facet |
Servetto, Natalia de Aranzamendi, M C Bettencourt, Raul Held, Christoph Abele, Doris Movilla, Juancho González, G Bustos, D M Sahade, Ricardo José |
author_sort |
Servetto, Natalia |
title |
Seawater carbonate chemistry and gene expression (RT-PCR) and enzyme activity of the Antarctic coral Malacobelemnon daytoni |
title_short |
Seawater carbonate chemistry and gene expression (RT-PCR) and enzyme activity of the Antarctic coral Malacobelemnon daytoni |
title_full |
Seawater carbonate chemistry and gene expression (RT-PCR) and enzyme activity of the Antarctic coral Malacobelemnon daytoni |
title_fullStr |
Seawater carbonate chemistry and gene expression (RT-PCR) and enzyme activity of the Antarctic coral Malacobelemnon daytoni |
title_full_unstemmed |
Seawater carbonate chemistry and gene expression (RT-PCR) and enzyme activity of the Antarctic coral Malacobelemnon daytoni |
title_sort |
seawater carbonate chemistry and gene expression (rt-pcr) and enzyme activity of the antarctic coral malacobelemnon daytoni |
publisher |
PANGAEA |
publishDate |
2021 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.936683 https://doi.org/10.1594/PANGAEA.936683 |
op_coverage |
LATITUDE: -62.233300 * LONGITUDE: -58.666700 |
long_lat |
ENVELOPE(-58.666700,-58.666700,-62.233300,-62.233300) |
genre |
Antarc* Antarctic Ocean acidification Southern Ocean |
genre_facet |
Antarc* Antarctic Ocean acidification Southern Ocean |
op_relation |
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html https://doi.pangaea.de/10.1594/PANGAEA.936683 https://doi.org/10.1594/PANGAEA.936683 |
op_rights |
CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1594/PANGAEA.936683 |
_version_ |
1810489387108007936 |