Phytoplankton species determination and counts determined from the community sampled at station West and East (PS97) before and after exposure to different Fe and Mn availabilities
Two Fe-Mn bottle amendment experiments with two natural phytoplankton communities were performed during Polarstern expedition PS97 in 2016 in the Drake Passage. At two locations, sea water was pumped (using trace metals clean techniques) from 25m depth and used to fill polycarbonate bottles after ha...
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.935864 2024-09-15T17:43:47+00:00 Phytoplankton species determination and counts determined from the community sampled at station West and East (PS97) before and after exposure to different Fe and Mn availabilities Balaguer, Jenna Koch, Florian Hassler, Christel S Trimborn, Scarlett MEDIAN LATITUDE: -59.641250 * MEDIAN LONGITUDE: -63.613335 * SOUTH-BOUND LATITUDE: -60.413170 * WEST-BOUND LONGITUDE: -66.361670 * NORTH-BOUND LATITUDE: -58.869330 * EAST-BOUND LONGITUDE: -60.865000 * DATE/TIME START: 2016-03-02T08:19:00 * DATE/TIME END: 2016-03-17T11:16:00 * MINIMUM ELEVATION: -3639.3 m * MAXIMUM ELEVATION: 0.0 m 2021 text/tab-separated-values, 180 data points https://doi.pangaea.de/10.1594/PANGAEA.935864 https://doi.org/10.1594/PANGAEA.935864 en eng PANGAEA https://doi.org/10.1594/PANGAEA.935868 Balaguer, Jenna; Koch, Florian; Hassler, Christel S; Trimborn, Scarlett (2022): Iron and manganese co-limit the growth of two phytoplankton groups dominant at two locations of the Drake Passage. Communications Biology, 5(1), 207, https://doi.org/10.1038/s42003-022-03148-8 https://doi.pangaea.de/10.1594/PANGAEA.935864 https://doi.org/10.1594/PANGAEA.935864 CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess ANT-XXXI/3 Chaetoceros sp. co-limitation Drake Passage Event label Experiment Experimental treatment Fragilariopsis sp. Incubation duration Light microscopy (Utermöhl 1958) Membrane pump MP Phaeocystis antarctica Phytoplankton composition Polarstern Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas PS97 PS97/043-1 PS97/087-4 Pseudo-nitzschia sp. Scotia Sea Southern Ocean SPP1158 trace elements dataset 2021 ftpangaea https://doi.org/10.1594/PANGAEA.93586410.1594/PANGAEA.93586810.1038/s42003-022-03148-8 2024-07-24T02:31:42Z Two Fe-Mn bottle amendment experiments with two natural phytoplankton communities were performed during Polarstern expedition PS97 in 2016 in the Drake Passage. At two locations, sea water was pumped (using trace metals clean techniques) from 25m depth and used to fill polycarbonate bottles after having passed through a cleaned 200 μm mesh (removing large grazers). The Control treatment was the sampled seawater without any trace metals addition while the other three treatments were enriched with either FeCl3 alone (0.5 nM; +Fe treatment) or MnCl2 alone (1 nM; +Mn treatment) or both trace metals together (+FeMn treatment). All treatments were done in triplicate 2,5L PC bottles. All incubation bottles were maintained at 30 μmol photons m-2 s-1 under a 16:8 (light:dark) hour cycle at 1 ̊C. To determine the effects of the different treatments on the microplankton composition for the two Fe-Mn enrichment experiments, unfiltered seawater was collected at the start and the end of both experiments for later analysis via light microscopy in the home laboratory. Briefly, samples were fixed with hexamine-buffered formalin solution (2% final concentration) and Lugol's solution (1% final concentration) and stored at 2 °C in the dark until taxonomic analysis. All samples were allowed to settle in Utermöhl sedimentation chambers for at least 24 hours and were analyzed on an inverted light microscope, according to the method of Utermöhl (1958). Species were counted and identified according to taxonomic literature. Each aliquot was examined until at least 400 cells had been counted. Dataset Antarc* Antarctic Antarctica Drake Passage Phytoplankton Scotia Sea Sea ice Southern Ocean PANGAEA - Data Publisher for Earth & Environmental Science ENVELOPE(-66.361670,-60.865000,-58.869330,-60.413170) |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
ANT-XXXI/3 Chaetoceros sp. co-limitation Drake Passage Event label Experiment Experimental treatment Fragilariopsis sp. Incubation duration Light microscopy (Utermöhl 1958) Membrane pump MP Phaeocystis antarctica Phytoplankton composition Polarstern Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas PS97 PS97/043-1 PS97/087-4 Pseudo-nitzschia sp. Scotia Sea Southern Ocean SPP1158 trace elements |
spellingShingle |
ANT-XXXI/3 Chaetoceros sp. co-limitation Drake Passage Event label Experiment Experimental treatment Fragilariopsis sp. Incubation duration Light microscopy (Utermöhl 1958) Membrane pump MP Phaeocystis antarctica Phytoplankton composition Polarstern Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas PS97 PS97/043-1 PS97/087-4 Pseudo-nitzschia sp. Scotia Sea Southern Ocean SPP1158 trace elements Balaguer, Jenna Koch, Florian Hassler, Christel S Trimborn, Scarlett Phytoplankton species determination and counts determined from the community sampled at station West and East (PS97) before and after exposure to different Fe and Mn availabilities |
topic_facet |
ANT-XXXI/3 Chaetoceros sp. co-limitation Drake Passage Event label Experiment Experimental treatment Fragilariopsis sp. Incubation duration Light microscopy (Utermöhl 1958) Membrane pump MP Phaeocystis antarctica Phytoplankton composition Polarstern Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas PS97 PS97/043-1 PS97/087-4 Pseudo-nitzschia sp. Scotia Sea Southern Ocean SPP1158 trace elements |
description |
Two Fe-Mn bottle amendment experiments with two natural phytoplankton communities were performed during Polarstern expedition PS97 in 2016 in the Drake Passage. At two locations, sea water was pumped (using trace metals clean techniques) from 25m depth and used to fill polycarbonate bottles after having passed through a cleaned 200 μm mesh (removing large grazers). The Control treatment was the sampled seawater without any trace metals addition while the other three treatments were enriched with either FeCl3 alone (0.5 nM; +Fe treatment) or MnCl2 alone (1 nM; +Mn treatment) or both trace metals together (+FeMn treatment). All treatments were done in triplicate 2,5L PC bottles. All incubation bottles were maintained at 30 μmol photons m-2 s-1 under a 16:8 (light:dark) hour cycle at 1 ̊C. To determine the effects of the different treatments on the microplankton composition for the two Fe-Mn enrichment experiments, unfiltered seawater was collected at the start and the end of both experiments for later analysis via light microscopy in the home laboratory. Briefly, samples were fixed with hexamine-buffered formalin solution (2% final concentration) and Lugol's solution (1% final concentration) and stored at 2 °C in the dark until taxonomic analysis. All samples were allowed to settle in Utermöhl sedimentation chambers for at least 24 hours and were analyzed on an inverted light microscope, according to the method of Utermöhl (1958). Species were counted and identified according to taxonomic literature. Each aliquot was examined until at least 400 cells had been counted. |
format |
Dataset |
author |
Balaguer, Jenna Koch, Florian Hassler, Christel S Trimborn, Scarlett |
author_facet |
Balaguer, Jenna Koch, Florian Hassler, Christel S Trimborn, Scarlett |
author_sort |
Balaguer, Jenna |
title |
Phytoplankton species determination and counts determined from the community sampled at station West and East (PS97) before and after exposure to different Fe and Mn availabilities |
title_short |
Phytoplankton species determination and counts determined from the community sampled at station West and East (PS97) before and after exposure to different Fe and Mn availabilities |
title_full |
Phytoplankton species determination and counts determined from the community sampled at station West and East (PS97) before and after exposure to different Fe and Mn availabilities |
title_fullStr |
Phytoplankton species determination and counts determined from the community sampled at station West and East (PS97) before and after exposure to different Fe and Mn availabilities |
title_full_unstemmed |
Phytoplankton species determination and counts determined from the community sampled at station West and East (PS97) before and after exposure to different Fe and Mn availabilities |
title_sort |
phytoplankton species determination and counts determined from the community sampled at station west and east (ps97) before and after exposure to different fe and mn availabilities |
publisher |
PANGAEA |
publishDate |
2021 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.935864 https://doi.org/10.1594/PANGAEA.935864 |
op_coverage |
MEDIAN LATITUDE: -59.641250 * MEDIAN LONGITUDE: -63.613335 * SOUTH-BOUND LATITUDE: -60.413170 * WEST-BOUND LONGITUDE: -66.361670 * NORTH-BOUND LATITUDE: -58.869330 * EAST-BOUND LONGITUDE: -60.865000 * DATE/TIME START: 2016-03-02T08:19:00 * DATE/TIME END: 2016-03-17T11:16:00 * MINIMUM ELEVATION: -3639.3 m * MAXIMUM ELEVATION: 0.0 m |
long_lat |
ENVELOPE(-66.361670,-60.865000,-58.869330,-60.413170) |
genre |
Antarc* Antarctic Antarctica Drake Passage Phytoplankton Scotia Sea Sea ice Southern Ocean |
genre_facet |
Antarc* Antarctic Antarctica Drake Passage Phytoplankton Scotia Sea Sea ice Southern Ocean |
op_relation |
https://doi.org/10.1594/PANGAEA.935868 Balaguer, Jenna; Koch, Florian; Hassler, Christel S; Trimborn, Scarlett (2022): Iron and manganese co-limit the growth of two phytoplankton groups dominant at two locations of the Drake Passage. Communications Biology, 5(1), 207, https://doi.org/10.1038/s42003-022-03148-8 https://doi.pangaea.de/10.1594/PANGAEA.935864 https://doi.org/10.1594/PANGAEA.935864 |
op_rights |
CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1594/PANGAEA.93586410.1594/PANGAEA.93586810.1038/s42003-022-03148-8 |
_version_ |
1810490958962229248 |