Chlorophyll a-content of all (total), the small (0.2-2 μm) and the large (> 2 μm) cells determined from the community sampled at station West and East (PS97) before and after exposure to different Fe and Mn availabilities
Two Fe-Mn bottle amendment experiments with two natural phytoplankton communities were performed during Polarstern expedition PS97 in 2016 in the Drake Passage. At two locations, sea water was pumped (using trace metals clean techniques) from 25m depth and used to fill polycarbonate bottles after ha...
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.935859 2024-09-15T17:43:23+00:00 Chlorophyll a-content of all (total), the small (0.2-2 μm) and the large (> 2 μm) cells determined from the community sampled at station West and East (PS97) before and after exposure to different Fe and Mn availabilities Balaguer, Jenna Koch, Florian Hassler, Christel S Trimborn, Scarlett MEDIAN LATITUDE: -59.641250 * MEDIAN LONGITUDE: -63.613335 * SOUTH-BOUND LATITUDE: -60.413170 * WEST-BOUND LONGITUDE: -66.361670 * NORTH-BOUND LATITUDE: -58.869330 * EAST-BOUND LONGITUDE: -60.865000 * DATE/TIME START: 2016-03-02T08:19:00 * DATE/TIME END: 2016-03-17T11:16:00 * MINIMUM ELEVATION: -3639.3 m * MAXIMUM ELEVATION: 0.0 m 2021 text/tab-separated-values, 164 data points https://doi.pangaea.de/10.1594/PANGAEA.935859 https://doi.org/10.1594/PANGAEA.935859 en eng PANGAEA https://doi.org/10.1594/PANGAEA.935868 Balaguer, Jenna; Koch, Florian; Hassler, Christel S; Trimborn, Scarlett (2022): Iron and manganese co-limit the growth of two phytoplankton groups dominant at two locations of the Drake Passage. Communications Biology, 5(1), 207, https://doi.org/10.1038/s42003-022-03148-8 https://doi.pangaea.de/10.1594/PANGAEA.935859 https://doi.org/10.1594/PANGAEA.935859 CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess ANT-XXXI/3 Calculated Chlorophyll a size fraction > 2 µm size fraction 0.2-2 µm total co-limitation Drake Passage Event label Experiment Experimental treatment Incubation duration Laboratory fluorometer Turner Trilogy Membrane pump MP Phytoplankton composition Polarstern Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas PS97 PS97/043-1 PS97/087-4 Scotia Sea Southern Ocean SPP1158 trace elements dataset 2021 ftpangaea https://doi.org/10.1594/PANGAEA.93585910.1594/PANGAEA.93586810.1038/s42003-022-03148-8 2024-07-24T02:31:42Z Two Fe-Mn bottle amendment experiments with two natural phytoplankton communities were performed during Polarstern expedition PS97 in 2016 in the Drake Passage. At two locations, sea water was pumped (using trace metals clean techniques) from 25m depth and used to fill polycarbonate bottles after having passed through a cleaned 200 μm mesh (removing large grazers). The Control treatment was the sampled seawater without any trace metals addition while the other three treatments were enriched with either FeCl3 alone (0.5 nM; +Fe treatment) or MnCl2 alone (1 nM; +Mn treatment) or both trace metals together (+FeMn treatment). All treatments were done in triplicate 2,5L PC bottles. All incubation bottles were maintained at 30 μmol photons m-2 s-1 under a 16:8 (light:dark) hour cycle at 1 ̊C. Chlorophyll a samples were taken at the beginning and the end of both experiments. In order to compare the contribution of large (>2 μm) relative to small cells (0.2-2 μm), 250 mL (on average) of samples were filtered onto 0.2 μm (for the total fraction) and 2 µm (for the large fraction) polycarbonate filters, hence the small fraction was calculated as the difference of the total and the large fraction. All samples were directly flash frozen into liquid nitrogen (N~2~) and then stored at −80 ̊C in the dark until further analysis. After being homogenized, samples were extracted in 90% acetone for 24h at 4 ̊C in the dark and analyzed fluorometrically on a Trilogy Fluorometer. Dataset Antarc* Antarctic Drake Passage Phytoplankton Scotia Sea Sea ice Southern Ocean PANGAEA - Data Publisher for Earth & Environmental Science ENVELOPE(-66.361670,-60.865000,-58.869330,-60.413170) |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
ANT-XXXI/3 Calculated Chlorophyll a size fraction > 2 µm size fraction 0.2-2 µm total co-limitation Drake Passage Event label Experiment Experimental treatment Incubation duration Laboratory fluorometer Turner Trilogy Membrane pump MP Phytoplankton composition Polarstern Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas PS97 PS97/043-1 PS97/087-4 Scotia Sea Southern Ocean SPP1158 trace elements |
spellingShingle |
ANT-XXXI/3 Calculated Chlorophyll a size fraction > 2 µm size fraction 0.2-2 µm total co-limitation Drake Passage Event label Experiment Experimental treatment Incubation duration Laboratory fluorometer Turner Trilogy Membrane pump MP Phytoplankton composition Polarstern Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas PS97 PS97/043-1 PS97/087-4 Scotia Sea Southern Ocean SPP1158 trace elements Balaguer, Jenna Koch, Florian Hassler, Christel S Trimborn, Scarlett Chlorophyll a-content of all (total), the small (0.2-2 μm) and the large (> 2 μm) cells determined from the community sampled at station West and East (PS97) before and after exposure to different Fe and Mn availabilities |
topic_facet |
ANT-XXXI/3 Calculated Chlorophyll a size fraction > 2 µm size fraction 0.2-2 µm total co-limitation Drake Passage Event label Experiment Experimental treatment Incubation duration Laboratory fluorometer Turner Trilogy Membrane pump MP Phytoplankton composition Polarstern Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas PS97 PS97/043-1 PS97/087-4 Scotia Sea Southern Ocean SPP1158 trace elements |
description |
Two Fe-Mn bottle amendment experiments with two natural phytoplankton communities were performed during Polarstern expedition PS97 in 2016 in the Drake Passage. At two locations, sea water was pumped (using trace metals clean techniques) from 25m depth and used to fill polycarbonate bottles after having passed through a cleaned 200 μm mesh (removing large grazers). The Control treatment was the sampled seawater without any trace metals addition while the other three treatments were enriched with either FeCl3 alone (0.5 nM; +Fe treatment) or MnCl2 alone (1 nM; +Mn treatment) or both trace metals together (+FeMn treatment). All treatments were done in triplicate 2,5L PC bottles. All incubation bottles were maintained at 30 μmol photons m-2 s-1 under a 16:8 (light:dark) hour cycle at 1 ̊C. Chlorophyll a samples were taken at the beginning and the end of both experiments. In order to compare the contribution of large (>2 μm) relative to small cells (0.2-2 μm), 250 mL (on average) of samples were filtered onto 0.2 μm (for the total fraction) and 2 µm (for the large fraction) polycarbonate filters, hence the small fraction was calculated as the difference of the total and the large fraction. All samples were directly flash frozen into liquid nitrogen (N~2~) and then stored at −80 ̊C in the dark until further analysis. After being homogenized, samples were extracted in 90% acetone for 24h at 4 ̊C in the dark and analyzed fluorometrically on a Trilogy Fluorometer. |
format |
Dataset |
author |
Balaguer, Jenna Koch, Florian Hassler, Christel S Trimborn, Scarlett |
author_facet |
Balaguer, Jenna Koch, Florian Hassler, Christel S Trimborn, Scarlett |
author_sort |
Balaguer, Jenna |
title |
Chlorophyll a-content of all (total), the small (0.2-2 μm) and the large (> 2 μm) cells determined from the community sampled at station West and East (PS97) before and after exposure to different Fe and Mn availabilities |
title_short |
Chlorophyll a-content of all (total), the small (0.2-2 μm) and the large (> 2 μm) cells determined from the community sampled at station West and East (PS97) before and after exposure to different Fe and Mn availabilities |
title_full |
Chlorophyll a-content of all (total), the small (0.2-2 μm) and the large (> 2 μm) cells determined from the community sampled at station West and East (PS97) before and after exposure to different Fe and Mn availabilities |
title_fullStr |
Chlorophyll a-content of all (total), the small (0.2-2 μm) and the large (> 2 μm) cells determined from the community sampled at station West and East (PS97) before and after exposure to different Fe and Mn availabilities |
title_full_unstemmed |
Chlorophyll a-content of all (total), the small (0.2-2 μm) and the large (> 2 μm) cells determined from the community sampled at station West and East (PS97) before and after exposure to different Fe and Mn availabilities |
title_sort |
chlorophyll a-content of all (total), the small (0.2-2 μm) and the large (> 2 μm) cells determined from the community sampled at station west and east (ps97) before and after exposure to different fe and mn availabilities |
publisher |
PANGAEA |
publishDate |
2021 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.935859 https://doi.org/10.1594/PANGAEA.935859 |
op_coverage |
MEDIAN LATITUDE: -59.641250 * MEDIAN LONGITUDE: -63.613335 * SOUTH-BOUND LATITUDE: -60.413170 * WEST-BOUND LONGITUDE: -66.361670 * NORTH-BOUND LATITUDE: -58.869330 * EAST-BOUND LONGITUDE: -60.865000 * DATE/TIME START: 2016-03-02T08:19:00 * DATE/TIME END: 2016-03-17T11:16:00 * MINIMUM ELEVATION: -3639.3 m * MAXIMUM ELEVATION: 0.0 m |
long_lat |
ENVELOPE(-66.361670,-60.865000,-58.869330,-60.413170) |
genre |
Antarc* Antarctic Drake Passage Phytoplankton Scotia Sea Sea ice Southern Ocean |
genre_facet |
Antarc* Antarctic Drake Passage Phytoplankton Scotia Sea Sea ice Southern Ocean |
op_relation |
https://doi.org/10.1594/PANGAEA.935868 Balaguer, Jenna; Koch, Florian; Hassler, Christel S; Trimborn, Scarlett (2022): Iron and manganese co-limit the growth of two phytoplankton groups dominant at two locations of the Drake Passage. Communications Biology, 5(1), 207, https://doi.org/10.1038/s42003-022-03148-8 https://doi.pangaea.de/10.1594/PANGAEA.935859 https://doi.org/10.1594/PANGAEA.935859 |
op_rights |
CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1594/PANGAEA.93585910.1594/PANGAEA.93586810.1038/s42003-022-03148-8 |
_version_ |
1810490356920221696 |