Seawater carbonate chemistry and the physiological performance of the chlorophyte Dunaliella tertiolecta
The effects of increased CO2 and irradiance on the physiological performance of the chlorophyte Dunaliella tertiolecta were studied at different PAR and UVR (UVA+UVB) irradiances, simulating the solar radiation at different depths, under present (390 ppmv, LC) and predicted CO2 levels for the year 2...
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.933580 2024-09-15T18:28:27+00:00 Seawater carbonate chemistry and the physiological performance of the chlorophyte Dunaliella tertiolecta García-Gómez, Candela Gordillo, Francisco J L Palma, Armando Lorenzo, M Rosario Segovia, María 2014 text/tab-separated-values, 696 data points https://doi.pangaea.de/10.1594/PANGAEA.933580 https://doi.org/10.1594/PANGAEA.933580 en eng PANGAEA García-Gómez, Candela; Gordillo, Francisco J L; Palma, Armando; Lorenzo, M Rosario; Segovia, María (2014): Elevated CO2 alleviates high PAR and UV stress in the unicellular chlorophyte Dunaliella tertiolecta. Photochemical & Photobiological Sciences, 13(9), 1347, https://doi.org/10.1039/c4pp00044g Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html https://doi.pangaea.de/10.1594/PANGAEA.933580 https://doi.org/10.1594/PANGAEA.933580 CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess Alkalinity total Aragonite saturation state Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Chlorophyta Cyclobutane pyrimidine dimers accumulation Dunaliella tertiolecta Experiment duration Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene expression Gene expression (incl. proteomics) Growth/Morphology Growth rate Irradiance Laboratory experiment Laboratory strains Light Macroalgae Maximum quantum yield of photosystem II standard deviation Not applicable OA-ICC Ocean Acidification International Coordination Centre Other studied parameter or process Partial pressure of carbon dioxide Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos pH Plantae Position Primary production/Photosynthesis Proliferating cell nuclear antigen accumulation Reactive oxygen species Registration number of species Replicate Repressor of silencing accumulation Salinity dataset 2014 ftpangaea https://doi.org/10.1594/PANGAEA.933580 2024-07-24T02:31:34Z The effects of increased CO2 and irradiance on the physiological performance of the chlorophyte Dunaliella tertiolecta were studied at different PAR and UVR (UVA+UVB) irradiances, simulating the solar radiation at different depths, under present (390 ppmv, LC) and predicted CO2 levels for the year 2100 (1000 ppmv, HC). Elevated CO2 resulted in higher optimum and effective quantum yields (Fv/Fm and Phi PSII, respectively), electron transport rates (ETR) and specific growth rates (µ). Cell stress was alleviated in HC respect to LC as evidenced by a decrease in reactive oxygen species (ROS) accumulation. DNA damage showed a 42-fold increase in cyclobutane-pyrimidine dimers (CPDs) formation under the highest irradiance (1100 µmol quanta/m**2/s) in LC with respect to the lowest irradiance (200 µmol quanta/m**2/s). Photolyase (CII-PCD-PL) gene expression was upregulated under HC resulting in a drastic decrease in CPDs accumulation to only 25% with respect to LC. Proliferating cell nuclear antigen (PCNA) accumulation was always higher in HC and the accumulation pattern indicated its involvement in repair or growth depending on the irradiance dose. The repressor of silencing (ROS1) was only marginally involved in the response, suggesting that photoreactivation was the most relevant mechanism to overcome UVR damage. Our results demonstrate that future scenarios of global change result in alleviation of irradiance stress by CO2–induced photoprotection in D. tertiolecta. Dataset Ocean acidification PANGAEA - Data Publisher for Earth & Environmental Science |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
Alkalinity total Aragonite saturation state Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Chlorophyta Cyclobutane pyrimidine dimers accumulation Dunaliella tertiolecta Experiment duration Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene expression Gene expression (incl. proteomics) Growth/Morphology Growth rate Irradiance Laboratory experiment Laboratory strains Light Macroalgae Maximum quantum yield of photosystem II standard deviation Not applicable OA-ICC Ocean Acidification International Coordination Centre Other studied parameter or process Partial pressure of carbon dioxide Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos pH Plantae Position Primary production/Photosynthesis Proliferating cell nuclear antigen accumulation Reactive oxygen species Registration number of species Replicate Repressor of silencing accumulation Salinity |
spellingShingle |
Alkalinity total Aragonite saturation state Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Chlorophyta Cyclobutane pyrimidine dimers accumulation Dunaliella tertiolecta Experiment duration Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene expression Gene expression (incl. proteomics) Growth/Morphology Growth rate Irradiance Laboratory experiment Laboratory strains Light Macroalgae Maximum quantum yield of photosystem II standard deviation Not applicable OA-ICC Ocean Acidification International Coordination Centre Other studied parameter or process Partial pressure of carbon dioxide Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos pH Plantae Position Primary production/Photosynthesis Proliferating cell nuclear antigen accumulation Reactive oxygen species Registration number of species Replicate Repressor of silencing accumulation Salinity García-Gómez, Candela Gordillo, Francisco J L Palma, Armando Lorenzo, M Rosario Segovia, María Seawater carbonate chemistry and the physiological performance of the chlorophyte Dunaliella tertiolecta |
topic_facet |
Alkalinity total Aragonite saturation state Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Chlorophyta Cyclobutane pyrimidine dimers accumulation Dunaliella tertiolecta Experiment duration Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Gene expression Gene expression (incl. proteomics) Growth/Morphology Growth rate Irradiance Laboratory experiment Laboratory strains Light Macroalgae Maximum quantum yield of photosystem II standard deviation Not applicable OA-ICC Ocean Acidification International Coordination Centre Other studied parameter or process Partial pressure of carbon dioxide Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos pH Plantae Position Primary production/Photosynthesis Proliferating cell nuclear antigen accumulation Reactive oxygen species Registration number of species Replicate Repressor of silencing accumulation Salinity |
description |
The effects of increased CO2 and irradiance on the physiological performance of the chlorophyte Dunaliella tertiolecta were studied at different PAR and UVR (UVA+UVB) irradiances, simulating the solar radiation at different depths, under present (390 ppmv, LC) and predicted CO2 levels for the year 2100 (1000 ppmv, HC). Elevated CO2 resulted in higher optimum and effective quantum yields (Fv/Fm and Phi PSII, respectively), electron transport rates (ETR) and specific growth rates (µ). Cell stress was alleviated in HC respect to LC as evidenced by a decrease in reactive oxygen species (ROS) accumulation. DNA damage showed a 42-fold increase in cyclobutane-pyrimidine dimers (CPDs) formation under the highest irradiance (1100 µmol quanta/m**2/s) in LC with respect to the lowest irradiance (200 µmol quanta/m**2/s). Photolyase (CII-PCD-PL) gene expression was upregulated under HC resulting in a drastic decrease in CPDs accumulation to only 25% with respect to LC. Proliferating cell nuclear antigen (PCNA) accumulation was always higher in HC and the accumulation pattern indicated its involvement in repair or growth depending on the irradiance dose. The repressor of silencing (ROS1) was only marginally involved in the response, suggesting that photoreactivation was the most relevant mechanism to overcome UVR damage. Our results demonstrate that future scenarios of global change result in alleviation of irradiance stress by CO2–induced photoprotection in D. tertiolecta. |
format |
Dataset |
author |
García-Gómez, Candela Gordillo, Francisco J L Palma, Armando Lorenzo, M Rosario Segovia, María |
author_facet |
García-Gómez, Candela Gordillo, Francisco J L Palma, Armando Lorenzo, M Rosario Segovia, María |
author_sort |
García-Gómez, Candela |
title |
Seawater carbonate chemistry and the physiological performance of the chlorophyte Dunaliella tertiolecta |
title_short |
Seawater carbonate chemistry and the physiological performance of the chlorophyte Dunaliella tertiolecta |
title_full |
Seawater carbonate chemistry and the physiological performance of the chlorophyte Dunaliella tertiolecta |
title_fullStr |
Seawater carbonate chemistry and the physiological performance of the chlorophyte Dunaliella tertiolecta |
title_full_unstemmed |
Seawater carbonate chemistry and the physiological performance of the chlorophyte Dunaliella tertiolecta |
title_sort |
seawater carbonate chemistry and the physiological performance of the chlorophyte dunaliella tertiolecta |
publisher |
PANGAEA |
publishDate |
2014 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.933580 https://doi.org/10.1594/PANGAEA.933580 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
García-Gómez, Candela; Gordillo, Francisco J L; Palma, Armando; Lorenzo, M Rosario; Segovia, María (2014): Elevated CO2 alleviates high PAR and UV stress in the unicellular chlorophyte Dunaliella tertiolecta. Photochemical & Photobiological Sciences, 13(9), 1347, https://doi.org/10.1039/c4pp00044g Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html https://doi.pangaea.de/10.1594/PANGAEA.933580 https://doi.org/10.1594/PANGAEA.933580 |
op_rights |
CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1594/PANGAEA.933580 |
_version_ |
1810469827169484800 |