Seawater carbonate chemistry and acid–base balance in the hæmolymph of European abalone (Haliotis tuberculata)
Ocean acidification (OA) and the associated changes in seawater carbonate chemistry pose a threat to calcifying organisms. This is particularly serious for shelled molluscs, in which shell growth and microstructure has been shown to be highly sensitive to OA. To improve our understanding of the resp...
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.932922 2024-09-15T18:28:07+00:00 Seawater carbonate chemistry and acid–base balance in the hæmolymph of European abalone (Haliotis tuberculata) Auzoux-Bordenave, Stephanie Chevret, Sandra Badou, Aïcha Martin, Sophie Di Giglio, Sarah Dubois, Philippe LATITUDE: 48.613800 * LONGITUDE: -4.600800 2021 text/tab-separated-values, 637 data points https://doi.pangaea.de/10.1594/PANGAEA.932922 https://doi.org/10.1594/PANGAEA.932922 en eng PANGAEA Auzoux-Bordenave, Stephanie; Chevret, Sandra; Badou, Aïcha; Martin, Sophie; Di Giglio, Sarah; Dubois, Philippe (2021): Acid–base balance in the hæmolymph of European abalone (Haliotis tuberculata) exposed to CO2-induced ocean acidification. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 259, 110996, https://doi.org/10.1016/j.cbpa.2021.110996 Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html https://doi.pangaea.de/10.1594/PANGAEA.932922 https://doi.org/10.1594/PANGAEA.932922 CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess Acid-base regulation Alkalinity total standard deviation Animalia Aragonite saturation state Benthic animals Benthos Bicarbonate ion Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Calculated using seacarb after Orr et al. (2018) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Coast and continental shelf Containers and aquaria (20-1000 L or < 1 m**2) EXP Experiment Experiment duration France_Haliotis_abalone_farm Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Fugacity of carbon dioxide in seawater Haemolymph dataset 2021 ftpangaea https://doi.org/10.1594/PANGAEA.932922 2024-07-24T02:31:34Z Ocean acidification (OA) and the associated changes in seawater carbonate chemistry pose a threat to calcifying organisms. This is particularly serious for shelled molluscs, in which shell growth and microstructure has been shown to be highly sensitive to OA. To improve our understanding of the responses of abalone to OA, this study investigated the effects of CO2-induced ocean acidification on extra-cellular acid–base parameters in the European abalone Haliotis tuberculata. Three-year-old adult abalone were exposed for 15 days to three different pH levels (7.9, 7.7, 7.4) representing current and predicted near-future conditions. Hæmolymph pH and total alkalinity were measured at different time points during exposure and used to calculate the carbonate parameters of the extracellular fluid. Total protein content was also measured to determine whether seawater acidification influences the composition and buffer capacity of hæmolymph. Extracellular pH was maintained at seawater pH 7.7 indicating that abalones are able to buffer moderate acidification (−0.2 pH units). This was not due to an accumulation of HCO3− ions but rather to a high hæmolymph protein concentration. By contrast, hæmolymph pH was significantly decreased after 5 days of exposure to pH 7.4, indicating that abalone do not compensate for higher decreases in seawater pH. Total alkalinity and dissolved inorganic carbon were also significantly decreased after 15 days of low pH exposure. It is concluded that changes in the acid–base balance of the hæmolymph might be involved in deleterious effects recorded in adult H. tuberculata facing severe OA stress. This would impact both the ecology and aquaculture of this commercially important species. Dataset Ocean acidification PANGAEA - Data Publisher for Earth & Environmental Science ENVELOPE(-4.600800,-4.600800,48.613800,48.613800) |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
Acid-base regulation Alkalinity total standard deviation Animalia Aragonite saturation state Benthic animals Benthos Bicarbonate ion Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Calculated using seacarb after Orr et al. (2018) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Coast and continental shelf Containers and aquaria (20-1000 L or < 1 m**2) EXP Experiment Experiment duration France_Haliotis_abalone_farm Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Fugacity of carbon dioxide in seawater Haemolymph |
spellingShingle |
Acid-base regulation Alkalinity total standard deviation Animalia Aragonite saturation state Benthic animals Benthos Bicarbonate ion Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Calculated using seacarb after Orr et al. (2018) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Coast and continental shelf Containers and aquaria (20-1000 L or < 1 m**2) EXP Experiment Experiment duration France_Haliotis_abalone_farm Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Fugacity of carbon dioxide in seawater Haemolymph Auzoux-Bordenave, Stephanie Chevret, Sandra Badou, Aïcha Martin, Sophie Di Giglio, Sarah Dubois, Philippe Seawater carbonate chemistry and acid–base balance in the hæmolymph of European abalone (Haliotis tuberculata) |
topic_facet |
Acid-base regulation Alkalinity total standard deviation Animalia Aragonite saturation state Benthic animals Benthos Bicarbonate ion Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Calculated using seacarb after Orr et al. (2018) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Coast and continental shelf Containers and aquaria (20-1000 L or < 1 m**2) EXP Experiment Experiment duration France_Haliotis_abalone_farm Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Fugacity of carbon dioxide in seawater Haemolymph |
description |
Ocean acidification (OA) and the associated changes in seawater carbonate chemistry pose a threat to calcifying organisms. This is particularly serious for shelled molluscs, in which shell growth and microstructure has been shown to be highly sensitive to OA. To improve our understanding of the responses of abalone to OA, this study investigated the effects of CO2-induced ocean acidification on extra-cellular acid–base parameters in the European abalone Haliotis tuberculata. Three-year-old adult abalone were exposed for 15 days to three different pH levels (7.9, 7.7, 7.4) representing current and predicted near-future conditions. Hæmolymph pH and total alkalinity were measured at different time points during exposure and used to calculate the carbonate parameters of the extracellular fluid. Total protein content was also measured to determine whether seawater acidification influences the composition and buffer capacity of hæmolymph. Extracellular pH was maintained at seawater pH 7.7 indicating that abalones are able to buffer moderate acidification (−0.2 pH units). This was not due to an accumulation of HCO3− ions but rather to a high hæmolymph protein concentration. By contrast, hæmolymph pH was significantly decreased after 5 days of exposure to pH 7.4, indicating that abalone do not compensate for higher decreases in seawater pH. Total alkalinity and dissolved inorganic carbon were also significantly decreased after 15 days of low pH exposure. It is concluded that changes in the acid–base balance of the hæmolymph might be involved in deleterious effects recorded in adult H. tuberculata facing severe OA stress. This would impact both the ecology and aquaculture of this commercially important species. |
format |
Dataset |
author |
Auzoux-Bordenave, Stephanie Chevret, Sandra Badou, Aïcha Martin, Sophie Di Giglio, Sarah Dubois, Philippe |
author_facet |
Auzoux-Bordenave, Stephanie Chevret, Sandra Badou, Aïcha Martin, Sophie Di Giglio, Sarah Dubois, Philippe |
author_sort |
Auzoux-Bordenave, Stephanie |
title |
Seawater carbonate chemistry and acid–base balance in the hæmolymph of European abalone (Haliotis tuberculata) |
title_short |
Seawater carbonate chemistry and acid–base balance in the hæmolymph of European abalone (Haliotis tuberculata) |
title_full |
Seawater carbonate chemistry and acid–base balance in the hæmolymph of European abalone (Haliotis tuberculata) |
title_fullStr |
Seawater carbonate chemistry and acid–base balance in the hæmolymph of European abalone (Haliotis tuberculata) |
title_full_unstemmed |
Seawater carbonate chemistry and acid–base balance in the hæmolymph of European abalone (Haliotis tuberculata) |
title_sort |
seawater carbonate chemistry and acid–base balance in the hæmolymph of european abalone (haliotis tuberculata) |
publisher |
PANGAEA |
publishDate |
2021 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.932922 https://doi.org/10.1594/PANGAEA.932922 |
op_coverage |
LATITUDE: 48.613800 * LONGITUDE: -4.600800 |
long_lat |
ENVELOPE(-4.600800,-4.600800,48.613800,48.613800) |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
Auzoux-Bordenave, Stephanie; Chevret, Sandra; Badou, Aïcha; Martin, Sophie; Di Giglio, Sarah; Dubois, Philippe (2021): Acid–base balance in the hæmolymph of European abalone (Haliotis tuberculata) exposed to CO2-induced ocean acidification. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 259, 110996, https://doi.org/10.1016/j.cbpa.2021.110996 Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html https://doi.pangaea.de/10.1594/PANGAEA.932922 https://doi.org/10.1594/PANGAEA.932922 |
op_rights |
CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1594/PANGAEA.932922 |
_version_ |
1810469433570754560 |