Specific RubisCO activity of currently uncultured microorganisms inhabiting hydrothermal deep sea vents

• Main Authors: Böhnke-Brandt, Stefanie, Perner, Mirjam
• Format: Dataset
• Language: English
• Published: PANGAEA 2021
• Subjects: Accession number; genetics; activity-based screen; Area/locality; autotrophic CO2-fixation; DATE/TIME; DERIDGE; ELEVATION; Event label; From Mantle to Ocean: Energy-; Material- and Life-cycles at Spreading Axes; functional metagenomic; Gaussian propagation of error; heat block; High Performance Liquid Chromatography (HPLC); HPLC; hydrothermal deep sea vents; Identification; LATITUDE; Linkage between the distribution and biochemical properties of RuBisCO and CODH enzymes and abiotic properties in thermally and chemically distinct deep-sea hydrothermal vent systems; LONGITUDE; M78/2; M78/2_274; M78/2_314; Maria S. Merian; MARSUED5; Meteor (1986); MSM10/3; MSM10/3_300; MSM10/3_313ROV-12; North Atlantic Ocean; Remote operated vehicle; ROV; RuBisCO; Sample code/label; Sample type; Sanger Sequencing modified after Sanger et al. (1977); PacBio Sequencing according to Rhoads and Au (2015); Site; Specific ribulose-1,5-bisphosphate carboxylase/oxygenase activity; standard deviation; specific RubisCO activities; Temperature; technical; Treatment; tropical/subtropical North Atlantic; North Atlantic
• Online Access: https://doi.pangaea.de/10.1594/PANGAEA.931785; https://doi.org/10.1594/PANGAEA.931785

In 2014 we described a new activity-based screen suited to seek active recombinant RubisCOs from the environment - independent of the native host's culturability. Within this study we aimed at seeking novel RubisCO active enzymes from the environment. For this purpose we screened six metagenomi...