Seawater carbonate chemistry and gene expression levels of the limpet Nacella concinna

• Main Authors: de Aranzamendi, M C, Servetto, Natalia, Movilla, Juancho, Bettencourt, Raul, Sahade, Ricardo José
• Format: Dataset
• Language: English
• Published: PANGAEA 2021
• Subjects: Alkalinity; total; standard deviation; Animalia; Antarctic; Aragonite saturation state; Benthic animals; Benthos; Bicarbonate ion; Bottles or small containers/Aquaria (<20 L); Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Calculated using seacarb after Orr et al. (2018); Carbon; inorganic; dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Coast and continental shelf; Comment; Dry air column-averaged mixing ratio of carbon dioxide; Ecotype; EXP; Experiment; Experiment day; Experiment duration; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Fugacity of carbon dioxide in seawater; Gene expression; Antarc*; Antarctica; Ocean acidification
• Online Access: https://doi.pangaea.de/10.1594/PANGAEA.930756; https://doi.org/10.1594/PANGAEA.930756

Ocean acidification (OA) could become a serious threat for the Antarctic marine ecosystem over coming years, as the solubility of atmospheric CO2 and CaCO3 minerals increases at lower temperatures. We evaluated the effect of OA on the stress response of the limpet Nacella concinna by measuring gene expression levels. The experiment was performed with the two ecotypes (Littoral and Sublittoral) of the species during 54 days (IPCC, 2019 scenario RCP8.5; control, 375 ppm; low-pH treatment, 923 ppm). Exposure to low-pH treatment during 15 days triggered the down-regulation of two heat-shock protein genes (HSP70A, HSP70B) only in sublittoral individuals. Little variation in the relative expression values of all genes in both ecotypes was observed probably, due to a historical exposure to the substantial daily natural pH fluctuations recorded in the study area during the experiment. This study provides relevant baseline data for future OA experiments on coastal species in Antarctica.