Seawater carbonate chemistry and polyspermy of a broadcast spawning bivalve
Ensuring that oocytes are fertilized by a single sperm during broadcast spawning is crucial for the fertilization success of many marine invertebrates. Although the adverse impacts of ocean acidification (OA) on various marine species have been revealed in recent years, its impact on polyspermy and...
Main Authors: | , , , , , , , |
---|---|
Format: | Dataset |
Language: | English |
Published: |
PANGAEA
2021
|
Subjects: | |
Online Access: | https://doi.pangaea.de/10.1594/PANGAEA.929201 https://doi.org/10.1594/PANGAEA.929201 |
id |
ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.929201 |
---|---|
record_format |
openpolar |
spelling |
ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.929201 2023-05-15T17:51:07+02:00 Seawater carbonate chemistry and polyspermy of a broadcast spawning bivalve Han, Yu Shi, Wei Tang, Y Zhao, Xinguo Du, Xueying Sun, Shuge Zhou, Weishang Liu, Guangxu LATITUDE: 28.283500 * LONGITUDE: 121.244300 2021-03-16 text/tab-separated-values, 3744 data points https://doi.pangaea.de/10.1594/PANGAEA.929201 https://doi.org/10.1594/PANGAEA.929201 en eng PANGAEA Han, Yu; Shi, Wei; Tang, Y; Zhao, Xinguo; Du, Xueying; Sun, Shuge; Zhou, Weishang; Liu, Guangxu (2021): Ocean acidification increases polyspermy of a broadcast spawning bivalve species by hampering membrane depolarization and cortical granule exocytosis. Aquatic Toxicology, 231, 105740, https://doi.org/10.1016/j.aquatox.2020.105740 Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html https://doi.pangaea.de/10.1594/PANGAEA.929201 https://doi.org/10.1594/PANGAEA.929201 CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess CC-BY Adenosine triphosphate per unit protein Alkalinity total standard deviation Animalia Aragonite saturation state Benthic animals Benthos Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Coast and continental shelf EXP Experiment Fluorescence intensity Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Laboratory experiment Microfilament migration Mollusca North Pacific OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) pH Polyspermy Potentiometric Potentiometric titration Dataset 2021 ftpangaea https://doi.org/10.1594/PANGAEA.929201 https://doi.org/10.1016/j.aquatox.2020.105740 2023-01-20T09:14:36Z Ensuring that oocytes are fertilized by a single sperm during broadcast spawning is crucial for the fertilization success of many marine invertebrates. Although the adverse impacts of ocean acidification (OA) on various marine species have been revealed in recent years, its impact on polyspermy and the underlying mechanisms involved remain largely unknown. Therefore, in the present study, the effect of OA on polyspermy risk was assessed in a broadcast spawning bivalve, Tegillarca granosa. In addition, the impacts of OA on the two polyspermy blocking processes, the fast block (membrane depolarization) and the permanent block (cortical reaction), were investigated. The results show that the exposure of oocytes to two future OA scenarios (pH 7.8 and pH 7.4) leads to significant increases in polyspermy risk, about 1.70 and 2.38 times higher than the control, respectively. The maximum change in the membrane potential during oocyte membrane depolarization markedly decreased to 15.79% (pH 7.8) and 34.06% (pH 7.4) of the control value. Moreover, the duration of oocyte membrane depolarization was significantly reduced to approximately 63.38% (pH 7.8) and 21.91% (pH 7.4) of the control. In addition, cortical granule exocytosis, as well as microfilament migration, were significantly arrested by OA treatment. Exposure to future OA scenarios also led to significant reductions in the ATP and Ca2+ content of the oocytes, which may explain the hampered polyspermy blocking. Overall, the present study suggests that OA may significantly increase polyspermy risk in T. granosa by inhibiting membrane depolarization and arresting cortical granule exocytosis. Dataset Ocean acidification PANGAEA - Data Publisher for Earth & Environmental Science Pacific ENVELOPE(121.244300,121.244300,28.283500,28.283500) |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
Adenosine triphosphate per unit protein Alkalinity total standard deviation Animalia Aragonite saturation state Benthic animals Benthos Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Coast and continental shelf EXP Experiment Fluorescence intensity Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Laboratory experiment Microfilament migration Mollusca North Pacific OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) pH Polyspermy Potentiometric Potentiometric titration |
spellingShingle |
Adenosine triphosphate per unit protein Alkalinity total standard deviation Animalia Aragonite saturation state Benthic animals Benthos Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Coast and continental shelf EXP Experiment Fluorescence intensity Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Laboratory experiment Microfilament migration Mollusca North Pacific OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) pH Polyspermy Potentiometric Potentiometric titration Han, Yu Shi, Wei Tang, Y Zhao, Xinguo Du, Xueying Sun, Shuge Zhou, Weishang Liu, Guangxu Seawater carbonate chemistry and polyspermy of a broadcast spawning bivalve |
topic_facet |
Adenosine triphosphate per unit protein Alkalinity total standard deviation Animalia Aragonite saturation state Benthic animals Benthos Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Coast and continental shelf EXP Experiment Fluorescence intensity Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Laboratory experiment Microfilament migration Mollusca North Pacific OA-ICC Ocean Acidification International Coordination Centre Partial pressure of carbon dioxide Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) pH Polyspermy Potentiometric Potentiometric titration |
description |
Ensuring that oocytes are fertilized by a single sperm during broadcast spawning is crucial for the fertilization success of many marine invertebrates. Although the adverse impacts of ocean acidification (OA) on various marine species have been revealed in recent years, its impact on polyspermy and the underlying mechanisms involved remain largely unknown. Therefore, in the present study, the effect of OA on polyspermy risk was assessed in a broadcast spawning bivalve, Tegillarca granosa. In addition, the impacts of OA on the two polyspermy blocking processes, the fast block (membrane depolarization) and the permanent block (cortical reaction), were investigated. The results show that the exposure of oocytes to two future OA scenarios (pH 7.8 and pH 7.4) leads to significant increases in polyspermy risk, about 1.70 and 2.38 times higher than the control, respectively. The maximum change in the membrane potential during oocyte membrane depolarization markedly decreased to 15.79% (pH 7.8) and 34.06% (pH 7.4) of the control value. Moreover, the duration of oocyte membrane depolarization was significantly reduced to approximately 63.38% (pH 7.8) and 21.91% (pH 7.4) of the control. In addition, cortical granule exocytosis, as well as microfilament migration, were significantly arrested by OA treatment. Exposure to future OA scenarios also led to significant reductions in the ATP and Ca2+ content of the oocytes, which may explain the hampered polyspermy blocking. Overall, the present study suggests that OA may significantly increase polyspermy risk in T. granosa by inhibiting membrane depolarization and arresting cortical granule exocytosis. |
format |
Dataset |
author |
Han, Yu Shi, Wei Tang, Y Zhao, Xinguo Du, Xueying Sun, Shuge Zhou, Weishang Liu, Guangxu |
author_facet |
Han, Yu Shi, Wei Tang, Y Zhao, Xinguo Du, Xueying Sun, Shuge Zhou, Weishang Liu, Guangxu |
author_sort |
Han, Yu |
title |
Seawater carbonate chemistry and polyspermy of a broadcast spawning bivalve |
title_short |
Seawater carbonate chemistry and polyspermy of a broadcast spawning bivalve |
title_full |
Seawater carbonate chemistry and polyspermy of a broadcast spawning bivalve |
title_fullStr |
Seawater carbonate chemistry and polyspermy of a broadcast spawning bivalve |
title_full_unstemmed |
Seawater carbonate chemistry and polyspermy of a broadcast spawning bivalve |
title_sort |
seawater carbonate chemistry and polyspermy of a broadcast spawning bivalve |
publisher |
PANGAEA |
publishDate |
2021 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.929201 https://doi.org/10.1594/PANGAEA.929201 |
op_coverage |
LATITUDE: 28.283500 * LONGITUDE: 121.244300 |
long_lat |
ENVELOPE(121.244300,121.244300,28.283500,28.283500) |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
Han, Yu; Shi, Wei; Tang, Y; Zhao, Xinguo; Du, Xueying; Sun, Shuge; Zhou, Weishang; Liu, Guangxu (2021): Ocean acidification increases polyspermy of a broadcast spawning bivalve species by hampering membrane depolarization and cortical granule exocytosis. Aquatic Toxicology, 231, 105740, https://doi.org/10.1016/j.aquatox.2020.105740 Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html https://doi.pangaea.de/10.1594/PANGAEA.929201 https://doi.org/10.1594/PANGAEA.929201 |
op_rights |
CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1594/PANGAEA.929201 https://doi.org/10.1016/j.aquatox.2020.105740 |
_version_ |
1766158147562504192 |