Seawater carbonate chemistry and sperm motility of Tegillarca granosavia
Since the industrial revolution, massive amount of anthropogenic carbon dioxide (CO2) have been generated to elevate the atmospheric CO2 concentration. Some anthropogenic CO2 have been absorbed by the ocean to cause “ocean acidification” (OA). Although the negative impacts of OA on sperm motility ar...
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2021
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.929195 2023-05-15T17:51:00+02:00 Seawater carbonate chemistry and sperm motility of Tegillarca granosavia Shi, Wei Han, Yu Huang, Xianke Hu, Lihua Chai, Xueliang Liu, Guangxu LATITUDE: 28.283500 * LONGITUDE: 121.244300 2021-03-16 text/tab-separated-values, 1263 data points https://doi.pangaea.de/10.1594/PANGAEA.929195 https://doi.org/10.1594/PANGAEA.929195 en eng PANGAEA Shi, Wei; Han, Yu; Huang, Xianke; Hu, Lihua; Chai, Xueliang; Liu, Guangxu (2020): Ocean acidification inhibits the sperm motility of Tegillarca granosavia disturbing ATP synthesis and intracellular CA2+ activity. Acta Hydrobiologica Sinica, 44, 570-576, https://doi.org/10.7541/2020.070 Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html https://doi.pangaea.de/10.1594/PANGAEA.929195 https://doi.org/10.1594/PANGAEA.929195 CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess CC-BY 6-phosphofructokinase activity per protein Adenosine triphosphate per unit protein Alkalinity total standard error Animalia Aragonite saturation state Average path velocity Behaviour Benthic animals Benthos Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calcium-ATPase activity Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Coast and continental shelf Curvilinear velocity EXP Experiment Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Laboratory experiment Mollusca North Pacific OA-ICC Ocean Acidification International Coordination Centre Other metabolic rates Dataset 2021 ftpangaea https://doi.org/10.1594/PANGAEA.929195 https://doi.org/10.7541/2020.070 2023-01-20T09:14:36Z Since the industrial revolution, massive amount of anthropogenic carbon dioxide (CO2) have been generated to elevate the atmospheric CO2 concentration. Some anthropogenic CO2 have been absorbed by the ocean to cause “ocean acidification” (OA). Although the negative impacts of OA on sperm motility are increasingly found in various marine invertebrate species, the cellular and molecular mechanisms for these effects are still poorly understood. This study investigated the effect of OA (pH7.8 and 7.4) on sperm motility and energy supplying pathway in blood clam Tegillarca granosa. The results showed that the sperm swimming speed reduced significantly in acidified seawater. Since the adenosine triphosphate (ATP) level of sperm is closely related to its motility, we analyzed the sperm ATP content and activities of key enzymes during ATP synthesis under different OA scenarios. OA treatments significantly reduced ATP content as well as activities of 6-phosphofructokinase and pyruvate kinase in the sperm of T. granosa. The sperm Ca2+-ATPase of various animals has been reported to regulate sperm motility. Therefore, we explored the Ca2+-ATPase activity of T. granosa sperm under OA treatment. The results found that Ca2+-ATPase activities in the sperm of T. granosa were significantly declined under OA scenarios. In conclusion, these results suggested that OA could constrain sperm motility through inhibiting ATP synthesis and disturbing intracellular Ca2+ regulation. Dataset Ocean acidification PANGAEA - Data Publisher for Earth & Environmental Science Pacific ENVELOPE(121.244300,121.244300,28.283500,28.283500) |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
6-phosphofructokinase activity per protein Adenosine triphosphate per unit protein Alkalinity total standard error Animalia Aragonite saturation state Average path velocity Behaviour Benthic animals Benthos Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calcium-ATPase activity Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Coast and continental shelf Curvilinear velocity EXP Experiment Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Laboratory experiment Mollusca North Pacific OA-ICC Ocean Acidification International Coordination Centre Other metabolic rates |
spellingShingle |
6-phosphofructokinase activity per protein Adenosine triphosphate per unit protein Alkalinity total standard error Animalia Aragonite saturation state Average path velocity Behaviour Benthic animals Benthos Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calcium-ATPase activity Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Coast and continental shelf Curvilinear velocity EXP Experiment Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Laboratory experiment Mollusca North Pacific OA-ICC Ocean Acidification International Coordination Centre Other metabolic rates Shi, Wei Han, Yu Huang, Xianke Hu, Lihua Chai, Xueliang Liu, Guangxu Seawater carbonate chemistry and sperm motility of Tegillarca granosavia |
topic_facet |
6-phosphofructokinase activity per protein Adenosine triphosphate per unit protein Alkalinity total standard error Animalia Aragonite saturation state Average path velocity Behaviour Benthic animals Benthos Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calcium-ATPase activity Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Coast and continental shelf Curvilinear velocity EXP Experiment Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Laboratory experiment Mollusca North Pacific OA-ICC Ocean Acidification International Coordination Centre Other metabolic rates |
description |
Since the industrial revolution, massive amount of anthropogenic carbon dioxide (CO2) have been generated to elevate the atmospheric CO2 concentration. Some anthropogenic CO2 have been absorbed by the ocean to cause “ocean acidification” (OA). Although the negative impacts of OA on sperm motility are increasingly found in various marine invertebrate species, the cellular and molecular mechanisms for these effects are still poorly understood. This study investigated the effect of OA (pH7.8 and 7.4) on sperm motility and energy supplying pathway in blood clam Tegillarca granosa. The results showed that the sperm swimming speed reduced significantly in acidified seawater. Since the adenosine triphosphate (ATP) level of sperm is closely related to its motility, we analyzed the sperm ATP content and activities of key enzymes during ATP synthesis under different OA scenarios. OA treatments significantly reduced ATP content as well as activities of 6-phosphofructokinase and pyruvate kinase in the sperm of T. granosa. The sperm Ca2+-ATPase of various animals has been reported to regulate sperm motility. Therefore, we explored the Ca2+-ATPase activity of T. granosa sperm under OA treatment. The results found that Ca2+-ATPase activities in the sperm of T. granosa were significantly declined under OA scenarios. In conclusion, these results suggested that OA could constrain sperm motility through inhibiting ATP synthesis and disturbing intracellular Ca2+ regulation. |
format |
Dataset |
author |
Shi, Wei Han, Yu Huang, Xianke Hu, Lihua Chai, Xueliang Liu, Guangxu |
author_facet |
Shi, Wei Han, Yu Huang, Xianke Hu, Lihua Chai, Xueliang Liu, Guangxu |
author_sort |
Shi, Wei |
title |
Seawater carbonate chemistry and sperm motility of Tegillarca granosavia |
title_short |
Seawater carbonate chemistry and sperm motility of Tegillarca granosavia |
title_full |
Seawater carbonate chemistry and sperm motility of Tegillarca granosavia |
title_fullStr |
Seawater carbonate chemistry and sperm motility of Tegillarca granosavia |
title_full_unstemmed |
Seawater carbonate chemistry and sperm motility of Tegillarca granosavia |
title_sort |
seawater carbonate chemistry and sperm motility of tegillarca granosavia |
publisher |
PANGAEA |
publishDate |
2021 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.929195 https://doi.org/10.1594/PANGAEA.929195 |
op_coverage |
LATITUDE: 28.283500 * LONGITUDE: 121.244300 |
long_lat |
ENVELOPE(121.244300,121.244300,28.283500,28.283500) |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
Shi, Wei; Han, Yu; Huang, Xianke; Hu, Lihua; Chai, Xueliang; Liu, Guangxu (2020): Ocean acidification inhibits the sperm motility of Tegillarca granosavia disturbing ATP synthesis and intracellular CA2+ activity. Acta Hydrobiologica Sinica, 44, 570-576, https://doi.org/10.7541/2020.070 Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html https://doi.pangaea.de/10.1594/PANGAEA.929195 https://doi.org/10.1594/PANGAEA.929195 |
op_rights |
CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1594/PANGAEA.929195 https://doi.org/10.7541/2020.070 |
_version_ |
1766157979010203648 |