Experimental conditions, respiration rates of permeabilized heart fibres and biometrical data of European seabass Dicentrarchus labrax

• Main Authors: Howald, Sarah, Cominassi, Louise, LeBayon, Nicolas, Claireaux, Guy, Mark, Felix Christopher
• Format: Dataset
• Language: English
• Published: PANGAEA 2020
• Subjects: Gulf of Morbihan; Ocean acidification
• Online Access: https://doi.pangaea.de/10.1594/PANGAEA.925193; https://doi.org/10.1594/PANGAEA.925193

The world's oceans are acidifying and warming as a result of increasing atmospheric CO2 concentrations. The thermal tolerance of fish greatly depends on the cardiovascular ability to supply the tissues with oxygen. The highly oxygen-dependent heart mitochondria thus might play a key role in shaping an organism's tolerance to temperature. The present study aimed to investigate the effects of acute and chronic warming on the respiratory capacity of European sea bass (Dicentrarchus labrax L.) heart mitochondria. Broodstock fish were caught in the Gulf of Morbihan, France. Larvae were raised at the aquaculture facility Aquastream (Ploemeur-Lorient, France) and obtained at 2 dph (20 January 2016). European sea bass were reared in the laboratory in six ocean acidification and warming (OAW) conditions: two temperatures (warm and cold life condition) and three PCO2 conditions (control, Δ500 and Δ1000). Conditions were chosen to follow the predictions of the IPCC for the next 130 years: ΔT = 5°C and ΔPCO2 = 500 and 1000 µatm, following RCP 6.0 and RCP 8.5 respectively. The fish were reared under these conditions from 3 dph (days post hatch) until mitochondrial respiration measurements at 3700 to 4100 dd (degree days, 183–199 dph and 234–249 dph in warm and cold life conditioned fish, respectively). During the experimental period, fish of all three PCO2 conditions of the respective temperature were used for mitochondrial respiration measurements on permeabilized heart fibres. Fish were not fed for 2 days prior to the experiments. Two batches of eight fish each were processed per day. Juveniles were randomly caught from their tanks and anesthetized with MS-222. Mass, fork length and body length were directly determined with a precision balance (Mettler, Columbus, OH, USA) and a calliper, to the nearest 0.01 g and 0.01 mm, respectively. Afterwards, fish were killed by a cut through the neck, and the heart was completely dissected from the fish, followed by excavation and permeabilization of the ventricle. Tissue from a ...